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2.
J Transl Med ; 21(1): 341, 2023 05 22.
Artigo em Inglês | MEDLINE | ID: mdl-37217923

RESUMO

BACKGROUND: Immunocheckpoint inhibitors (ICIs) have been widely used in the clinical treatment of lung cancer. Although clinical studies and trials have shown that patients can benefit significantly after PD-1/PD-L1 blocking therapy, less than 20% of patients can benefit from ICIs therapy due to tumor heterogeneity and the complexity of immune microenvironment. Several recent studies have explored the immunosuppression of PD-L1 expression and activity by post-translational regulation. Our published articles demonstrate that ISG15 inhibits lung adenocarcinoma progression. Whether ISG15 can enhance the efficacy of ICIs by modulating PD-L1 remains unknown. METHODS: The relationship between ISG15 and lymphocyte infiltration was identified by IHC. The effects of ISG15 on tumor cells and T lymphocytes were assessed using RT-qPCR and Western Blot and in vivo experiments. The underlying mechanism of PD-L1 post-translational modification by ISG15 was revealed by Western blot, RT-qPCR, flow cytometry, and Co-IP. Finally, we performed validation in C57 mice as well as in lung adenocarcinoma tissues. RESULTS: ISG15 promotes the infiltration of CD4+ T lymphocytes. In vivo and in vitro experiments demonstrated that ISG15 induces CD4+ T cell proliferation and invalidity and immune responses against tumors. Mechanistically, we demonstrated that the ubiquitination-like modifying effect of ISG15 on PD-L1 increased the modification of K48-linked ubiquitin chains thus increasing the degradation rate of glycosylated PD-L1 targeting proteasomal pathway. The expression of ISG15 and PD-L1 was negatively correlated in NSCLC tissues. In addition, reduced accumulation of PD-L1 by ISG15 in mice also increased splenic lymphocyte infiltration as well as promoted cytotoxic T cell infiltration in the tumor microenvironment, thereby enhancing anti-tumor immunity. CONCLUSIONS: The ubiquitination modification of PD-L1 by ISG15 increases K48-linked ubiquitin chain modification, thereby increasing the degradation rate of glycosylated PD-L1-targeted proteasome pathway. More importantly, ISG15 enhanced the sensitivity to immunosuppressive therapy. Our study shows that ISG15, as a post-translational modifier of PD-L1, reduces the stability of PD-L1 and may be a potential therapeutic target for cancer immunotherapy.


Assuntos
Adenocarcinoma de Pulmão , Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Animais , Camundongos , Antígeno B7-H1/metabolismo , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Linhagem Celular Tumoral , Neoplasias Pulmonares/patologia , Microambiente Tumoral , Ubiquitinas
3.
Front Plant Sci ; 14: 1120064, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37008462

RESUMO

Changes in net ecosystem productivity (NEP) in terrestrial ecosystems in response to climate warming and land cover changes have been of great concern. In this study, we applied the normalized difference vegetation index (NDVI), average temperature, and sunshine hours to drive the C-FIX model and to simulate the regional NEP in China from 2000 to 2019. We also analyzed the spatial patterns and the spatiotemporal variation characteristics of the NEP of terrestrial ecosystems and discussed their main influencing factors. The results showed that (1) the annual average NEP of terrestrial ecosystems in China from 2000 to 2019 was 1.08 PgC, exhibiting a highly significant increasing trend with a rate of change of 0.83 PgC/10 y. The terrestrial ecosystems in China remained as carbon sinks from 2000 to 2019, and the carbon sink capacity increased significantly. The NEP of the terrestrial ecosystem increased by 65% during 2015-2019 compared to 2000-2004 (2) There was spatial differences in the NEP distribution of the terrestrial ecosystems in China from 2000-2019. Taking the line along the Daxinganling-Yin Mountains-Helan Mountains-Transverse Range as the boundary, the NEP was significantly higher in the eastern part than in the western part. Among them, the NEP was positive (carbon sink) in northeastern, central, and southern China, and negative (carbon source) in parts of northwestern China and the Tibet Autonomous Region. The spatial variation of NEP in terrestrial ecosystems increased from 2000 to 2009. The areas with a significant increase accounted for 45.85% and were mainly located in the central and southwestern regions. (3) The simulation results revealed that vegetation changes and CO2 concentration changes both contributed to the increase in the NEP in China, contributing 85.96% and 36.84%, respectively. The vegetation changes were the main factor causing the increase in the NEP. The main contribution of this study is to further quantify the NEP of terrestrial ecosystems in China and identify the influencing factors that caused these changes.

4.
Sci Rep ; 13(1): 3879, 2023 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-36890155

RESUMO

The accurate delineation of the spatial extent of cold regions provides the basis for the study of global environmental change. However, attention has been lacking on the temperature-sensitive spatial changes in the cold regions of the Earth in the context of climate warming. In this study, the mean temperature in the coldest month lower than - 3 °C, no more than 5 months over 10 °C, and an annual mean temperature no higher than 5 °C were selected to define cold regions. Based on the Climate Research Unit land surface air temperature (CRUTEM) of monthly mean surface climate elements, the spatiotemporal distribution and variation characteristics of the Northern Hemisphere (NH) continental cold regions from 1901 to 2019 are analyzed in this study, by adopting time trend and correlation analyses. The results show: (1) In the past 119 years, the cold regions of the NH covered on average about 4.074 × 107 km2, accounting for 37.82% of the total land area of the NH. The cold regions can be divided into the Mid-to-High latitude cold regions and the Qinghai-Tibetan Plateau cold regions, with spatial extents of 3.755 × 107 km2 and 3.127 × 106 km2, respectively. The Mid-to-High latitude cold regions in the NH are mainly distributed in northern North America, most of Iceland, the Alps, northern Eurasia, and the Great Caucasus with a mean southern boundary of 49.48° N. Except for the southwest, the entire region of the Qinghai-Tibetan Plateau, northern Pakistan, and most of Kyrgyzstan are cold regions. (2) In the past 119 years, the rates of change in the spatial extent of the cold regions in the NH, the Mid-to-High latitude, and the Qinghai-Tibetan Plateau were - 0.030 × 107 km2/10 a, - 0.028 × 107 km2/10 a, and - 0.013 × 106 km2/10 a, respectively, showing an extremely significant decreasing trend. In the past 119 years, the mean southern boundary of the Mid-to-High latitude cold regions has been retreating northward at all longitudes. For instance, the mean southern boundary of the Eurasian cold regions moved 1.82° to the north and that of North America moved 0.98° to the north. The main contribution of the study lies in the accurate definition of the cold regions and documentation of the spatial variation of the cold regions in the NH, revealing the response trends of the cold regions to climate warming, and deepening the study of global change from a new perspective.

5.
Bioresour Technol ; 364: 128060, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36195217

RESUMO

This study aimed to evaluate differences in humic substance (HS) formation based on the shikimic acid pathway (SAP) during five different materials composting. The results showed that compared with other three materials, gallic acid, protocatechuic acid and shikimic acid of the SAP products in lawn waste (LW) and garden waste (GW) compost decreased significantly. Furthermore, as important indicators for evaluating humification, humic acid and degree of polymerization increased by 39.4%, 79.5% and 21.8%, 87.9% in LW and GW, respectively. Correlation analysis showed that SAP products were strongly correlated with HS fractions in LW and GW. Meanwhile, network analysis indicated that more core bacteria associated with both SAP products and HS were identified in LW and GW. Finally, the structural equation model proved that SAP had more significant contribution to humification improvement in LW and GW. These findings provided theoretical foundation and feasible actions to improve compost quality by the SAP.

6.
Bioresour Technol ; 360: 127579, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35798167

RESUMO

Metabolites of shikimic acid (SA) pathway can be used as humic substance (HS) precursors. Due to the complexity of SA anabolism, there were few studies on SA pathway during composting. The aim of this study was to identify the key drivers of SA pathway during different materials composting. During composting, the SA, protocatechuic acid (PA) and gallic acid (GA) decreased by 57.09%, 72.27% and 54.04% on average, respectively. The structural equation model showed that SA had key driving factors (organic matter and pH) during lawn waste composting. In addition, the complexity of material structure was the main factor affecting PA driving factors. The factors and degree of influence on GA varied with different materials. Accordingly, this study provided theoretical support for the improvement of SA metabolic intensity by single material and mixed material composting, and further provided a new direction for future HS research.


Assuntos
Compostagem , Substâncias Húmicas , Esterco , Ácido Chiquímico , Solo
7.
J Biotechnol ; 348: 55-63, 2022 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-35304164

RESUMO

Chitosanase was widely used in the production of bioactive chitooligosacchride (CHOS) due to their safety, controllability, environmental protection, and biodegradability. Studies showed that the bioactivity of CHOS is closely related to its degree of polymerization. Therefore, the production of ideal polymerized CHOS becomes our primary goal. In this study, the glycosyl hydrolase (GH) family 5 chitosanase was successfully expressed heterologously in Pichia pastoris. After 96 h of high-density fermentation, the chitosanase activity reached 90.62 U·mL-1, the protein content reached 9.76 mg·mL-1. When 2% chitosan was hydrolyzed by crude enzyme (20 U/mL), the hydrolysis rate reached 91.2% after 8 h, producing a mixture of CHOS with 2-4 desirable degrees of polymerization (DP). Then, the antioxidant activity of CHOS mixture was investigated, and the results showed that the antioxidant effect was concentration-dependent and had great application potential in the field of nutrition.


Assuntos
Quitosana , Saccharomycetales , Antioxidantes , Quitosana/metabolismo , Glicosídeo Hidrolases/genética , Glicosídeo Hidrolases/metabolismo , Hidrólise , Pichia/genética , Pichia/metabolismo , Saccharomycetales/metabolismo
8.
Soft Matter ; 18(8): 1644-1652, 2022 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-35128552

RESUMO

Developing multifunctional hydrogels with stretchability, self-healing ability, adhesiveness, and conductivity into flexible strain sensors for human motion and health monitoring has attracted great attention and is highly desired. However, the present motion detectors mainly focus on stretching, bending, and twisting of different body parts while the expansion-contraction motion has been rarely investigated. In this study, along with carbon nanotubes (CNTs) as conductive components, sodium alginate (Alg) modified with 3-aminophenylboronic acid (PBA) and dopamine (DA) were synthesized and employed as precursors to prepare a multifunctional Alg-CNT hydrogel. The formed dynamic covalent bonds between PBA and DA endowed the hydrogel with a rapid self-healing property (30 s) while the introduction of CNTs remarkably enhanced the mechanical strength and electrical conductivity of the hydrogel. Moreover, the as-prepared hydrogel displayed a satisfactory stretchability (500%) and self-adhesiveness to various substrates. When used as a strain sensor, the Alg-CNT hydrogel that exhibited a fast response (150 ms) and ultra-durability (over 30 000 cycles) was demonstrated to be capable of monitoring subtle expansion-contraction motions (e.g., human breathing and mouse heart beating) via periodic and repeatable electrical signals. Therefore, this multifunctional hydrogel is highly suitable for monitoring expansion-contraction motions, indicating its potential applications in personal health monitoring.


Assuntos
Nanotubos de Carbono , Dispositivos Eletrônicos Vestíveis , Adesivos , Alginatos , Animais , Condutividade Elétrica , Hidrogéis/química , Camundongos , Nanotubos de Carbono/química
9.
Carbohydr Polym ; 273: 118609, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34561008

RESUMO

Chitooligosaccharides (CHOS) with multiple biological activities are usually produced through enzymatic hydrolysis of chitosan or chitin. However, purification and recycling of the enzyme have largely limited the advancement of CHOS bioproduction. Here, we engineered a novel enzyme by fusing the native chitosanase Csn75 with a carbohydrate-binding module (CBM) that can specifically bind to curdlan. The recombinase Csn75-CBM was successfully expressed by Pichia pastoris and allowed one-step purification and immobilization in the chitosanase immobilized curdlan packed-bed reactor (CICPR), where a maximum adsorption capacity of 39.59 mg enzyme/g curdlan was achieved. CHOS with degrees of polymerization of 2-5 (a hydrolysis yield of 97.75%), 3-6 (75.45%), and 3-7 (73.2%) were continuously produced by adjusting the ratio of enzyme and chitosan or the flow rate of chitosan. Moreover, the CICPR exhibited good stability and reusability after several cycles. The recombinase Csn75-CBM has greatly improved the efficiency of the bioproduction of CHOS.


Assuntos
Quitosana/síntese química , Enzimas Imobilizadas/química , Glucana 1,3-beta-Glucosidase/química , Glicosídeo Hidrolases/química , Oligossacarídeos/síntese química , Aspergillus fumigatus/enzimologia , Bacillus/enzimologia , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Enzimas Imobilizadas/genética , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Glucana 1,3-beta-Glucosidase/genética , Glicosídeo Hidrolases/genética , Mutação , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/genética , Domínios Proteicos/genética , Engenharia de Proteínas , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/genética , beta-Glucanas
10.
Cancer Biol Med ; 2021 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-33710816

RESUMO

OBJECTIVE: The immunoscore, which is used to quantify immune infiltrates, has greater relative prognostic value than tumor, node, and metastasis (TNM) stage and might serve as a new system for classification of colorectal cancer. However, a comparable immunoscore for predicting lung adenocarcinoma (LUAD) prognosis is currently lacking. METHODS: We analyzed the expression of 18 immune features by immunohistochemistry in 171 specimens. The relationship of immune marker expression and clinicopathologic factors to the overall survival (OS) was analyzed with the Kaplan-Meier method. A nomogram was developed by using the optimal features selected by least absolute shrinkage and selection operator (LASSO) regression in the training cohort (n = 111) and evaluated in the validation cohort (n = 60). RESULTS: The indicators integrated in the nomogram were TNM stage, neuron-specific enolase, carcino-embryonic antigen, CD8center of tumor (CT), CD8invasive margin (IM), FoxP3CT, and CD45ROCT. The calibration curve showed prominent agreement between the observed 2- and 5-year OS and that predicted by the nomogram. To simplify the nomogram, we developed a new immune-serum scoring system (I-SSS) based on the points awarded for each factor in the nomogram. Our I-SSS was able to stratify same-stage patients into different risk subgroups. The combination of I-SSS and TNM stage had better prognostic value than the TNM stage alone. CONCLUSIONS: Our new I-SSS can accurately and individually predict LUAD prognosis and may be used to supplement prognostication based on the TNM stage.

11.
Phys Rev E ; 103(2-1): 023213, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33735973

RESUMO

The formation and structure of a collisional shock wave in a fully ionized plasma is studied via full particle-in-cell simulations, which allows the complex momentum and energy transfer processes between different charged particles to be treated self-consistently. The kinetic energy of the plasma flow drifting towards a reflecting piston is found to be rapidly converted into thermal motion under the cooperative effects of ion-ion collisions, ion-electron collisions, and electric field charged-particle interactions. The subsequent shock evolution is influenced by the "precursor" ion beam before a quasisteady state is reached. The shock wave structure is then analyzed from a two-fluid transport viewpoint, which is found to be affected by "flux-limiting" electron transport, the nonthermal ions, and the charge separation electric field.

12.
Virchows Arch ; 478(3): 449-458, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32918598

RESUMO

To examine the clinicopathologic and immunohistochemical features of a group of newly defined low-grade oncocytic renal tumors (LOT) that have the "CD117 negative/cytokeratin (CK)7 positive" immunoprofile. We have queried our hospital database and found 4456 consecutive renal tumors between 2016 and 2019. Among these renal tumors, eight (8) cases meet the morphologic and immunohistochemical characterization for low-grade oncocytic renal tumor (LOT). The eight (8) patients' mean age is 56.6 years (range 39-70 years old), and the male to female ratio is 1:1. Macroscopically, these LOTs generally present with tan-brown and solid cut surfaces and demonstrate similar solid, compact nested growth pattern microscopically. Tumor cells exhibit oncocytic cytoplasm and uniformly rounded to oval nuclei. There are areas of edematous stroma containing dispersed single or small clustered tumor cells. All tumors are negative for CD117 and positive for CK7. Uniform reactivity is also found for BerEP4, cyclin D1, and SDHB. Besides, CD10, vimentin, and AMACR are either negative or only focally positive. All of the tumors are negative for CA9 and TFE. The Ki-67 index is less than 5% in the seven (7) internal cases. Seven (7) of the eight (8) patients who are available for follow-up are alive and without disease recurrence (mean follow-up period of 21.6 months, ranging from 6 to 43 months). We described a group of low-grade oncocytic renal tumors identified retrospectively in a large tertiary cancer center, which was probably the first report originated from China or even Asia in the English literature so far. These tumors demonstrated eosinophilic cytoplasm and low-grade appearing nuclei with a "CD117 negative/CK7 positive" immunoprofile. The incidence rate was about 3.7% of the oncocytic renal tumors and 0.18% of all the renal tumors that were received in our lab during the four-year period. It is necessary to separate this group of tumors by its characteristic morphologic and immunophenotypic features.


Assuntos
Adenoma Oxífilo/química , Biomarcadores Tumorais/análise , Queratina-7/análise , Neoplasias Renais/química , Proteínas Proto-Oncogênicas c-kit/análise , Adenoma Oxífilo/patologia , Adenoma Oxífilo/cirurgia , Adulto , Idoso , China , Bases de Dados Factuais , Feminino , Humanos , Imuno-Histoquímica , Imunofenotipagem , Neoplasias Renais/patologia , Neoplasias Renais/cirurgia , Masculino , Pessoa de Meia-Idade , Gradação de Tumores , Estudos Retrospectivos , Centros de Atenção Terciária
13.
Cell Death Dis ; 11(7): 511, 2020 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-32641707

RESUMO

Our previous work demonstrated that Epithelial Splicing Regulatory Protein 1 (ESRP1) could inhibit the progression of lung adenocarcinoma (ADC). When ESRP1 was upregulated, the interferon (IFN) pathway was activated and Interferon-stimulated gene 15 (ISG15) expression increased exponentially in our microarray result. In this study, we aim to explore the function of ISG15 and its interactions with ESRP1 and to provide new insights for ADC treatment. ISG15 expression in lung ADC tissues was determined by immunohistochemistry (IHC) staining. The effect of ISG15 on lung ADC progression was examined by in vitro and in vivo assays. The mechanism of action on ESRP1 regulating ISG15 was investigated using Western blotting, RT-qPCR, immunofluorescence staining, chromatin immunoprecipitation, and a dual luciferase reporter system. The ISGylation between ISG15 and ESRP1 was detected by co-immunoprecipitation. Patients with high ISG15 expression were associated with higher survival rates, especially those with ISG15 expression in the nucleus. In vitro and in vivo experiments showed that upregulation of ISG15 inhibited EMT in lung ADC. ESRP1 upregulated the expression of ISG15 through CREB with enriched ISG15 in the nucleus. Importantly, ISG15 promoted ISGylation of ESRP1 and slowed the degradation of ESRP1, which demonstrated that ESRP1 and ISG15 formed a positive feedback loop and jointly suppressed EMT of lung ADC. In conclusion, ISG15 serves as an independent prognostic marker for long-term survival in lung ADC patients. We have revealed the protective effect of ISG15 against lung ADC progression and the combinatorial benefit of ISG15 and ESRP1 on inhibiting EMT. These findings suggest that reconstituting ISG15 and ESRP1 may have the potential for treating lung ADC.


Assuntos
Adenocarcinoma de Pulmão/metabolismo , Adenocarcinoma de Pulmão/patologia , Citocinas/metabolismo , Progressão da Doença , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Proteínas de Ligação a RNA/metabolismo , Ubiquitinas/metabolismo , Animais , Linhagem Celular Tumoral , Núcleo Celular/metabolismo , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo , Citocinas/genética , Transição Epitelial-Mesenquimal/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Interferons/metabolismo , Masculino , Camundongos Endogâmicos BALB C , Camundongos Nus , Pessoa de Meia-Idade , Análise Multivariada , Prognóstico , Modelos de Riscos Proporcionais , Proteólise , Transcrição Gênica , Ubiquitinas/genética , Regulação para Cima/genética
14.
J Infect Dis ; 222(5): 746-754, 2020 08 04.
Artigo em Inglês | MEDLINE | ID: mdl-32563194

RESUMO

Coronavirus disease 2019 (COVID-19) is an emerging infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We investigated the serum cytokine and chemokine levels in asymptomatic, mild, moderate, severe, and convalescent SARS-CoV-2-infected cases. Proinflammatory cytokine and chemokine production induced by SARS-CoV-2 were observed not only in symptomatic patients but also in asymptomatic cases, and returned to normal after recovery. IL-6, IL-7, IL-10, IL-18, G-CSF, M-CSF, MCP-1, MCP-3, IP-10, MIG, and MIP-1α were found to be associated with the severity of COVID-19. Moreover, a set of cytokine and chemokine profiles were significantly higher in SARS-CoV-2-infected male than female patients. The serum levels of MCP-1, G-CSF, and VEGF were weakly and positively correlated with viral titers. We suggest that combinatorial analysis of serum cytokines and chemokines with clinical classification may contribute to evaluation of the severity of COVID-19 and optimize the therapeutic strategies.


Assuntos
Quimiocinas/sangue , Infecções por Coronavirus/sangue , Citocinas/sangue , Pneumonia Viral/sangue , Adulto , Betacoronavirus/isolamento & purificação , COVID-19 , Quimiocina CCL2/sangue , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/virologia , Feminino , Fator Estimulador de Colônias de Granulócitos/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Pandemias , Pneumonia Viral/diagnóstico , Pneumonia Viral/imunologia , Pneumonia Viral/virologia , SARS-CoV-2 , Índice de Gravidade de Doença , Fator A de Crescimento do Endotélio Vascular/sangue , Carga Viral
15.
Nanotechnology ; 31(11): 115707, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-31747640

RESUMO

Photoelectrochemical water oxidation for hydrogen generation via utilizing sunlight is considered a very promising pathway for generating sustainable energy in an environmental manner. Here, a composite photoanode, consisting of nanopyramidal BiVO4 arrays and one layered double hydroxide (NiMn-LDH) was designed and fabricated via a facile route. The obtained BiVO4/NiMn-LDH composite photoelectrode presented a significant enhancement in the photoelectrochemical (PEC) current density, conversion efficiency and stability for solar water oxidation. With 2D NiMn-LDH decoration, an obvious cathodic shift of ∼480 mV in the onset potential can be observed, and more than two times enhancement in photocurrent performance is achieved. The improvement in photoelectrochemical activity for BiVO4/NiMn-LDH composite photoanode can be attributed to the enhanced water-oxidation kinetics leading to the efficient separation, transfer and collection of charge carriers at the photoanode/electrolyte interface. The result demonstrates NiMn-LDH represents one of the active oxygen evolution catalysts (OECs) to improve the PEC activity of metal oxide photoanode.

16.
Bioresour Technol ; 285: 121326, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30986627

RESUMO

This study aimed to explore the roles of bacterial community in the transformation of bioavailable organic-N (BON) during different wastes composting. BON fractions with different forms and molecular weights were identified in this study. Results indicated that core bacterial communities improved the availability of BON by degrading high molecular weights BON into low molecular weights BON during different wastes composting. A total of fifty-two core bacterial genera involved in BON transformation were identified by network analysis. Three types of high molecular weights BON fractions (amino acid-N, amine-N and amino sugar-N) were degraded by bacteria during chicken manure and garden waste composting, while only amine-N was degraded during municipal solid waste composting. Finally, moisture, C/N and pH were identified as the key operational parameters affecting BON transformation mediated by microorganisms, which can be used to improve bioavailability of organic-N and reduce N loss during composting.


Assuntos
Compostagem , Bactérias , Disponibilidade Biológica , Esterco , Nitrogênio , Solo
17.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 35(1): 78-82, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30914117

RESUMO

Objective To select and express single-chain fragment variable (scFv) against the envelope glycoprotein Gn of severe fever with thrombocytopenia syndrome virus (SFTSV). Methods Specific anti-SFTSV-Gn antibodies were isolated from scFv library against SFTSV after four rounds of "adsorption-elution-amplification" using SFTSV-Gn protein as immobilized antigen. Monoclonal phage enzyme linked immunosorbent assay (phage-ELISA) was performed for the phage antibodies on randomly picked single colonies from the screened library. The positive scFv against SFTSV-Gn protein was transformed into the BL21 E.coli cells for its prokaryotic expression, which was identified by Western blot analysis. The binding activity of purified scFv was tested by ELISA. Results Three different scFv specific for SFTSV-Gn protein were obtained and further expressed in BL21(DE3). Western blot analysis confirmed the prokaryotic expression of the anti-SFTSV-Gn antibody. ELISA demonstrated that the anti-SFTSV-Gn scFv had binding affinity with SFTSV-Gn. Conclusion The scFv against SFTSV has been selected and expressed successfully.


Assuntos
Phlebovirus , Trombocitopenia , Anticorpos Monoclonais , Ensaio de Imunoadsorção Enzimática , Glicoproteínas , Humanos
18.
Opt Express ; 26(18): 23705-23713, 2018 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-30184867

RESUMO

We examine the Goos-Hänchen (GH) effect for a Gaussian beam impinging on the surface of silicene whose topological phase transitions can be modulated by external electric field and/or irradiating circular polarized light. It is shown that both the spatial and angular shifts in GH effect present a sharp jump due to the topological phase transitions. The transitional GH effect can be attributed to transitional optical conductivity, which relates to Berry curvature and Chern numbers. These results can be extensively extended to other two-dimensional atomic crystals in graphene family. We believe that the transitional GH effect may offer a possible way to determine the Berry curvature, Chern numbers, and topological phase transition by a direct optical measurement.

19.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 34(5): 390-394, 2018 May.
Artigo em Chinês | MEDLINE | ID: mdl-30043728

RESUMO

Objective To construct a human phage display library against severe fever with thrombocytopenia syndrome (SFTS) virus. Methods The total RNA was isolated from peripheral blood lymphocytes of 8 patients with SFTS and cDNA was amplified. The genes of light-chain (Vκ and Vλ) and heavy-chain (VH) were amplified by PCR. The scFv gene was linked by overlap-PCR and cloned into the vector pComb3XSS, and then transformed into XL1-Blue cells for the phage antibody library construction. After detecting the recombinant rate and the library repertoire, we screened the antibodies against SFTS virus by biopanning with immobilized virus antigen. Results The volume of constructed phage antibody library was 2.8×107. The results of sequencing showed that the sequence of the contained single-chain variable fragment (scFv) was different. After three rounds of panning, phage antibodies were specifically enriched. A total of 21 clones were positive against SFTS virus by phage-ELISA. Conclusion A high-capacity and diverse human scFv against SFTS virus has been constructed successfully.


Assuntos
Biblioteca de Peptídeos , Phlebovirus , Anticorpos de Cadeia Única/química , Ensaio de Imunoadsorção Enzimática , Humanos
20.
Opt Lett ; 42(20): 4135-4138, 2017 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-29028031

RESUMO

We propose a simple method for the precise identification of graphene layers at the air-prism interface via a pseudo-Brewster angle, where the horizontally polarized reflection is close to zero. We find that the pseudo-Brewster angle is sensitive to the variation of graphene layers where the pseudo-Brewster angle is approximately linearly increased about 0.5 deg as the layer numbers increased. Furthermore, the sensitivity of the pseudo-Brewster angle can be greatly enhanced and reaches 0.04 deg by eliminating the influence of the cross-polarization effect. Our scheme can provide a simple and effective method to identify the layer numbers of graphene.

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