Esculetin improves murine mastitis induced by streptococcus isolated from bovine mammary glands by inhibiting NF-κB and MAPK signaling pathways.

Cow mastitis, caused by Streptococcus infection of the mammary glands, is a common clinical disease that can lead to decreased milk quality and threaten animal welfare and performance. Esculetin (ESC) is a coumarin with anti-inflammatory and anti-asthmatic effects. However, whether ESC has therapeutic effects on mastitis remains unexplored. This study was conducted to investigate the protective effect of ESC against murine mastitis caused by Streptococcus isolated from bovine mammary glands and elucidate the underlying mechanisms. Streptococcus uberis was used to construct a mouse model of mastitis. The results showed that the mice exhibited edema and thickening of the acinar wall with inflammatory infiltration after S. uberis treatment. Intraperitoneal injection of ESC significantly reduced inflammatory cell infiltration, restored normal physiological function, and inhibited the production of the inflammatory cytokines interleukin-1ß, interleukin-6, and tumor necrosis factor-α. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot analysis revealed that ESC reduced P38 phosphorylation, further inhibited the influence of mammary Streptococcus on cytoplasmic translocation of nuclear factor-κB (P65), and inhibited the transcriptional activation of P65, thus inhibiting the generation of inflammatory cells. Collectively, ESC may inhibit mitogen-activated protein kinase and nuclear factor-κB, thereby highlighting its potential for the treatment and prevention of mastitis.

A FRET aptasensor for sensitive detection of aflatoxin B1 based on a novel donor-acceptor pair between ZnS quantum dots and Ag nanocubes.

Aflatoxin B1 (AFB1) is one of the most carcinogenic chemicals. A novel fluorescence resonance energy transfer (FRET) sensor based on aptamer recognition technology is proposed for the sensitive detection of AFB1 in moldy peanuts using Ag nanocubes as energy acceptors and ZnS quantum dots (QDs) as energy donors. Compared to the traditional FRET system based on an Au quencher, Ag nanocubes can not only quench the fluorescence of aptamer modified ZnS QDs, but are also inexpensive. In addition, compared with heavy metal QDs, ZnS QDs are environmentally friendly, have excellent photochemical properties, and are ideal energy donors. Without Ag nanocubes, the aptamer modified ZnS QDs emits blue fluorescence under an ultraviolet lamp. Because the emission spectrum of ZnS and the absorption spectrum of Ag nanocubes meet the requirements of FRET, the fluorescence quenching of ZnS QDs is realized. Nevertheless, with AFB1, the specific binding of aptamer and complementary chain makes the ZnS QDs break away from the Ag nanocubes, which leads to the fluorescence recovery of the ZnS QDs. Under the optimized detection conditions, the linear range of AFB1 was 5 pg mL-1 to 300 ng mL-1, and there was no obvious reaction with other similar mycotoxins. According to S/N = 3, the detection limit of AFB1 was 2.67 pg mL-1. The detection of AFB1 in peanut samples shows that the new FRET system can successfully be applied in the future to agricultural products.