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1.
Braz J Med Biol Res ; 52(5): e7992, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31038546

RESUMO

The aim of this study was to evaluate the influence of artesunate on Th1 differentiation and its anti-tumor effect on ovarian cancer. A Murine ovarian cancer model was established by ID8 cells transplantation. The expression of miR-142 and Sirt1 proteins in peripheral CD4+ T cells were quantified with qRT-PCR and western blot, respectively. Peripheral CD4+ T cells were induced for Th1 differentiation. The percentages of apoptosis of Th1/CD4+ T cells and ovarian cancer cells were analyzed by flow cytometry. The IFN-γ level was examined through enzyme-linked immunosorbent assay. Artesunate promoted miR-142 expression in peripheral CD4+ T cells and Th1 differentiation from CD4+ T cells. Artesunate promoted cell apoptosis of ovarian cancer cells by inducing Th1 differentiation. By up-regulating miR-142, artesunate suppressed Sirt1 level and promoted Th1 differentiation. Artesunate enhanced the pro-apoptotic effects of Th1 cells on ovarian cancer via the miR-142/Sirt1 pathway. Artesunate promoted Th1 differentiation from CD4+ T cells by down-regulating Sirt1 through miR-142, thereby enhancing cell apoptosis in ovarian cancer.


Assuntos
Apoptose , Artesunato/farmacologia , Linfócitos T CD4-Positivos/efeitos dos fármacos , MicroRNAs/metabolismo , Neoplasias Ovarianas/tratamento farmacológico , Células Th1/efeitos dos fármacos , Animais , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Artesunato/uso terapêutico , Linfócitos T CD4-Positivos/citologia , Diferenciação Celular , Regulação para Baixo , Feminino , Citometria de Fluxo , Camundongos , Camundongos Endogâmicos C57BL , Neoplasias Ovarianas/imunologia , Células Th1/citologia
2.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;52(5): e7992, 2019. graf
Artigo em Inglês | LILACS | ID: biblio-1001527

RESUMO

The aim of this study was to evaluate the influence of artesunate on Th1 differentiation and its anti-tumor effect on ovarian cancer. A Murine ovarian cancer model was established by ID8 cells transplantation. The expression of miR-142 and Sirt1 proteins in peripheral CD4+ T cells were quantified with qRT-PCR and western blot, respectively. Peripheral CD4+ T cells were induced for Th1 differentiation. The percentages of apoptosis of Th1/CD4+ T cells and ovarian cancer cells were analyzed by flow cytometry. The IFN-γ level was examined through enzyme-linked immunosorbent assay. Artesunate promoted miR-142 expression in peripheral CD4+ T cells and Th1 differentiation from CD4+ T cells. Artesunate promoted cell apoptosis of ovarian cancer cells by inducing Th1 differentiation. By up-regulating miR-142, artesunate suppressed Sirt1 level and promoted Th1 differentiation. Artesunate enhanced the pro-apoptotic effects of Th1 cells on ovarian cancer via the miR-142/Sirt1 pathway. Artesunate promoted Th1 differentiation from CD4+ T cells by down-regulating Sirt1 through miR-142, thereby enhancing cell apoptosis in ovarian cancer.


Assuntos
Animais , Feminino , Coelhos , Neoplasias Ovarianas/tratamento farmacológico , Linfócitos T CD4-Positivos/efeitos dos fármacos , Apoptose , Células Th1/efeitos dos fármacos , MicroRNAs/metabolismo , Artesunato/farmacologia , Neoplasias Ovarianas/imunologia , Linfócitos T CD4-Positivos/citologia , Regulação para Baixo , Diferenciação Celular , Células Th1/citologia , Citometria de Fluxo , Artesunato/uso terapêutico , Camundongos Endogâmicos C57BL , Antineoplásicos/uso terapêutico , Antineoplásicos/farmacologia
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