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1.
Life Sci ; : 122893, 2024 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-38971367

RESUMO

AIMS: Neonatal necrotizing enterocolitis (NEC) is a leading cause of intestine inflammatory disease, and macrophage is significantly activated during NEC development. Posttranslational modifications (PTMs) of proteins, particularly ubiquitination, play critical roles in immune response. This study aimed to investigate the effects of ubiquitin-modified proteins on macrophage activation and NEC, and discover novel NEC-related inflammatory proteins. MATERIALS AND METHODS: Proteomic and ubiquitin proteomic analyses of intestinal macrophages in NEC/healthy mouse pups were carried out. In vitro macrophage inflammation model and in vivo NEC mouse model, as well as clinical human samples were used for further verification the inhibitor of nuclear factor-κB kinase α (IKKα) ubiquitination on NEC development through Western blot, immunofluorescence, quantitative real-time polymerase chain reaction (qRT-PCR) and flow cytometry. KEY FINDINGS: We report here that IKKα was a new ubiquitin-modified protein during NEC through ubiquitin proteomics, and RING finger protein 31 (RNF31) acted as an E3 ligase to be involved in IKKα degradation. Inhibition of IKKα ubiquitination and degradation with siRNF31 or proteasome inhibitor decreased nuclear factor-κB (NF-κB) activation, thereby decreasing the expression of pro-inflammatory factors and M1 macrophage polarization, resulting in reliving the severity of NEC. SIGNIFICANCE: Our study suggests the activation of RNF31-IKKα-NF-κB axis triggering NEC development and suppressing RNF31-mediated IKKα degradation may be therapeutic strategies to be developed for NEC treatment.

2.
Plant Cell Environ ; 46(10): 3090-3101, 2023 10.
Artigo em Inglês | MEDLINE | ID: mdl-36788431

RESUMO

Herbivore-induced plant defence responses share common components with plant responses to abiotic stresses. However, whether abiotic stress-responsive factors influence the resistance of plants to herbivores by regulating these components remains largely unknown. Here, we cloned a dehydration-responsive element-binding gene in rice, OsDREB1A, and investigated its role in the resistance of rice to the phloem-feeding herbivore, brown planthopper (BPH, Nilaparvata lugens), under normal and low temperatures. We found that OsDREB1A localized to the nucleus, and its transcripts in rice were up-regulated in response to BPH infestation, low temperatures and treatment with methyl jasmonate or salicylic acid. Silencing OsDREB1A changed transcript levels of two defence-related WRKY and two PLD genes, enhanced levels of jasmonic acid (JA), JA-isoleucine and abscisic acid, and decreased the ethylene level in rice; these changes subsequently enhanced the resistance of plants to BPH, especially at 17°C, by decreasing the hatching rate and delaying the development of BPH eggs. Moreover, silencing OsDREB1A increased the growth of rice plants. These findings suggest that OsDREB1A, which positively regulates the resistance of rice to abiotic stresses, negatively regulates the resistance of rice to BPH.


Assuntos
Hemípteros , Oryza , Animais , Ciclopentanos/farmacologia , Desidratação , Regulação da Expressão Gênica de Plantas , Hemípteros/fisiologia , Herbivoria , Oryza/fisiologia , Oxilipinas/farmacologia , Floema/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/metabolismo
3.
Front Cell Infect Microbiol ; 12: 1001607, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36699719

RESUMO

Objectives: To develop a rapid and low-cost method for 16S rDNA nanopore sequencing. Methods: This was a prospective study on a 16S rDNA nanopore sequencing method. We developed this nanopore barcoding 16S sequencing method by adding barcodes to the 16S primer to reduce the reagent cost and simplify the experimental procedure. Twenty-one common pulmonary bacteria (7 reference strains, 14 clinical isolates) and 94 samples of bronchoalveolar lavage fluid from children with severe pneumonia were tested. Results indicating low-abundance pathogenic bacteria were verified with the polymerase chain reaction (PCR). Further, the results were compared with those of culture or PCR. Results: The turnaround time was shortened to 6~8 hours and the reagent cost of DNA preparation was reduced by employing a single reaction adding barcodes to the 16S primer in advance. The accuracy rate for the 21 common pulmonary pathogens with an abundance ≥ 99% was 100%. Applying the culture or PCR results as the gold standard, 71 (75.5%) of the 94 patients were positive, including 25 positive cultures (26.6%) and 52 positive quantitative PCRs (55.3%). The median abundance in the positive culture and qPCR samples were 29.9% and 6.7%, respectively. With an abundance threshold increase of 1%, 5%, 10%, 15% and 20%, the test sensitivity decreased gradually to 98.6%, 84.9%, 72.6%, 67.1% and 64.4%, respectively, and the test specificity increased gradually to 33.3%, 71.4%, 81.0%, 90.5% and 100.0%, respectively. Conclusions: The nanopore barcoding 16S sequencing method can rapidly identify the pathogens causing bacterial pneumonia in children.


Assuntos
Sequenciamento por Nanoporos , Pneumonia Bacteriana , Humanos , Criança , DNA Ribossômico/genética , Estudos Prospectivos , DNA Bacteriano/genética , DNA Bacteriano/análise , Bactérias , Pneumonia Bacteriana/diagnóstico , RNA Ribossômico 16S/genética
4.
Int J Mol Sci ; 22(23)2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34884830

RESUMO

The RING-type E3 ubiquitin ligases play an important role in plant growth, development, and defense responses to abiotic stresses and pathogens. However, their roles in the resistance of plants to herbivorous insects remain largely unknown. In this study, we isolated the rice gene OsJMJ715, which encodes a RING-domain containing protein, and investigated its role in rice resistance to brown planthopper (BPH, Nilaparvata lugens). OsJMJ715 is a nucleus-localized E3 ligase whose mRNA levels were upregulated by the infestation of gravid BPH females, mechanical wounding, and treatment with JA or ABA. Silencing OsJMJ715 enhanced BPH-elicited levels of ABA, JA, and JA-Ile as well as the amount of callose deposition in plants, which in turn increased the resistance of rice to BPH by reducing the feeding of BPH and the hatching rate of BPH eggs. These findings suggest that OsJMJ715 negative regulates the BPH-induced biosynthesis of ABA, JA, and JA-Ile and that BPH benefits by enhancing the expression of OsJMJ715.


Assuntos
Ácido Abscísico/metabolismo , Ciclopentanos/metabolismo , Hemípteros/fisiologia , Oryza/metabolismo , Oxilipinas/metabolismo , Proteínas de Plantas/genética , Ubiquitina-Proteína Ligases/genética , Ácido Abscísico/farmacologia , Animais , Ciclopentanos/farmacologia , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Glucanos/metabolismo , Herbivoria , Isoleucina/análogos & derivados , Isoleucina/metabolismo , Oryza/crescimento & desenvolvimento , Oryza/parasitologia , Oxilipinas/farmacologia , Folhas de Planta/metabolismo , Folhas de Planta/parasitologia , Proteínas de Plantas/antagonistas & inibidores , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Plantas Geneticamente Modificadas/parasitologia , Interferência de RNA , RNA Mensageiro/metabolismo , Transdução de Sinais/efeitos dos fármacos , Ubiquitina-Proteína Ligases/antagonistas & inibidores , Ubiquitina-Proteína Ligases/metabolismo
5.
Ying Yong Sheng Tai Xue Bao ; 31(7): 2151-2160, 2020 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-32715676

RESUMO

There are complex chemical interactions between plants and phytophagous insects. On the one hand, when infested by phytophagous insects, plants can recognize herbivore-associated molecular patterns and trigger early signaling events and phytohormone-mediated signaling pathways. The activated signaling pathways thus result in the reconfiguration of transcriptomes and metabolomes as well as the increases in direct and indirect defensive compounds in plants, which in turn enhance the resistance of plants to phytophagous insects. On the other hand, phytophagous insects can recognize defense responses in plants and then inhibit or adapt to plant chemical defenses by secreting effector, sequestrating and detoxifying defensive compounds, and/or reducing sensitivity to defensive compounds. The deep analysis of chemical interactions between plant and phytophagous insects could improve the understanding of the relationship between insects and plants in theory and also provide important theoretical and technical guidance for the development of new technologies for crop pest control in practice.


Assuntos
Insetos , Plantas , Animais , Herbivoria , Controle de Pragas , Reguladores de Crescimento de Plantas
6.
Int J Mol Sci ; 20(12)2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31226870

RESUMO

Plants undergo several but very precise molecular, physiological, and biochemical modulations in response to biotic stresses. Mitogen-activated protein kinase (MAPK) cascades orchestrate multiple cellular processes including plant growth and development as well as plant responses against abiotic and biotic stresses. However, the role of MAPK kinases (MAPKKs/MKKs/MEKs) in the regulation of plant resistance to herbivores has not been extensively investigated. Here, we cloned a rice MKK gene, OsMKK3, and investigated its function. It was observed that mechanical wounding, infestation of brown planthopper (BPH) Nilaparvata lugens, and treatment with methyl jasmonate (MeJA) or salicylic acid (SA) could induce the expression of OsMKK3. The over-expression of OsMKK3 (oe-MKK3) increased levels of jasmonic acid (JA), jasmonoyl-L-isoleucine (JA-Ile), and abscisic acid (ABA), and decreased SA levels in rice after BPH attack. Additionally, the preference for feeding and oviposition, the hatching rate of BPH eggs, and BPH nymph survival rate were significantly compromised due to over-expression of OsMKK3. Besides, oe-MKK3 also augmented chlorophyll content but impaired plant growth. We confirm that MKK3 plays a pivotal role in the signaling pathway. It is proposed that OsMKK3 mediated positive regulation of rice resistance to BPH by means of herbivory-induced phytohormone dynamics.


Assuntos
Hemípteros/fisiologia , Herbivoria , MAP Quinase Quinase 3/metabolismo , Oryza/fisiologia , Proteínas de Plantas/metabolismo , Ácido Abscísico/metabolismo , Animais , Ciclopentanos/metabolismo , Isoleucina/análogos & derivados , Isoleucina/metabolismo , Oxilipinas/metabolismo , Reguladores de Crescimento de Plantas/metabolismo
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