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1.
Microb Pathog ; 187: 106511, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38168552

RESUMO

Mycoplasma gallisepticum (MG) infection causes infectious respiratory diseases in poultry, causing economic losses to the poultry industry. Therefore, this study aims to develop a safe, convenient, and effective multivalent recombinant Saccharomyces cerevisiae vaccine candidate and to explore its potential for oral immunization as a subunit vaccine. Mycoplasma gallisepticum Cytadhesin (MGC) and variable lipoprotein and hemagglutinin (vlhA) are associated with the pathogenesis of MG. In this study, a quadrivalent recombinant Saccharomyces cerevisiae (ST1814G-MG) displaying on MGC2, MGC3, VLH5, and VLH3, proteins was innovatively constructed, and its protective efficiency was evaluated in birds. The results showed that oral immunization with ST1814G-MG stimulates specific antibodies in chickens, reshapes the composition of the gut microbiota, reduces the Mycoplasma loading and pulmonary disease injury in the lungs. In addition, we found that oral ST1814G-MG had better protection against MG infection than an inactivated vaccine, and co-administration with the inactivated vaccine was even more effective. The results suggest that ST1814G-MG is a potentially safer and effective agent for controlling MG infection.


Assuntos
Microbioma Gastrointestinal , Infecções por Mycoplasma , Mycoplasma gallisepticum , Doenças das Aves Domésticas , Infecções Respiratórias , Animais , Galinhas , Mycoplasma gallisepticum/genética , Hemaglutininas , Saccharomyces cerevisiae/genética , Infecções por Mycoplasma/prevenção & controle , Infecções por Mycoplasma/veterinária , Anticorpos Antibacterianos , Doenças das Aves Domésticas/prevenção & controle , Vacinas de Produtos Inativados , Vacinas Bacterianas
2.
Vet Microbiol ; 271: 109490, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35709627

RESUMO

Hydropericardium-hepatitis syndrome (HHS) caused by fowl adenovirus (FAdV) serotype 4 strains is a highly contagious disease that causes significant economic loss to the global poultry industry. However, subunit vaccine against FAdV-4 infection is not yet commercially available to date. This study aims to explore the potential for oral immunization of recombinant Saccharomyces cerevisiae expressing Fiber-2 of FAdV-4 as a subunit vaccine. Here, we constructed recombinant S. cerevisiae (ST1814G/Fiber-2) expressing recombinant Fiber-2 (rFiber-2), which was displayed on the cell surface. To evaluate the immune response and protective effect of live recombinant S. cerevisiae, chickens were orally immunized with the constructed live ST1814G/Fiber-2, three times at 5-day intervals, and then challenged with FAdV-4. The results showed that oral administration of live ST1814G/Fiber-2 could stimulate the production of humoral immunity, enhance the body's antiviral activity and immune regulation ability, improve the composition of gut microbiota, provide protection against FAdV-4 challenge, reduce viral load in the liver, and alleviate the pathological damage of heart, liver, and spleen for chicken. In addition, we found the synergistic effect in combining the ST1814G/Fiber-2 yeast and inactivated vaccine to trigger stronger humoral immunity and mucosal immunity. Our results suggest that oral live ST1814G/Fiber-2 is a potentially safer auxiliary preparation strategy in controlling FAdV-4 infection.


Assuntos
Infecções por Adenoviridae , Aviadenovirus , Doenças das Aves Domésticas , Vacinas Virais , Adenoviridae , Infecções por Adenoviridae/prevenção & controle , Infecções por Adenoviridae/veterinária , Animais , Anticorpos Antivirais , Aviadenovirus/genética , Galinhas , Imunização/veterinária , Saccharomyces cerevisiae/genética , Sorogrupo , Vacinas de Subunidades Antigênicas
3.
Bioresour Technol ; 179: 144-149, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25541381

RESUMO

Propyzamide is a widely used benzamide herbicide for controlling weeds in lettuce, soybeans, cotton and other crops. An efficient propyzamide-degrading strain W1 was firstly isolated from activated sludge and identified as Comamonas testosteroni. A metabolite of propyzamide by strain W1 was firstly identified. The novel gene camH encoding a hydrolase that catalyzed the amide bond cleavage of propyzamide was cloned from strain W1. The gene contained an open reading frame of 1452 bp, the deduced amino acid sequence showed low identity with other amidases. The recombinant enzyme CamH was expressed in Escherichia coli BL21 and purified. CamH displayed the highest activity at 30°C and pH 8.0 with propyzamide as the substrate. These results provide important knowledge on the fate of propyzamide in the biodegradation, and elucidate the biodegradation mechanism of propyzamide by the strain W1.


Assuntos
Amidoidrolases/genética , Benzamidas/metabolismo , Comamonas testosteroni/enzimologia , Comamonas testosteroni/genética , Genes Bacterianos , Amidoidrolases/metabolismo , Biodegradação Ambiental/efeitos dos fármacos , Clonagem Molecular , Comamonas testosteroni/isolamento & purificação , Meios de Cultura/química , Eletroforese em Gel de Poliacrilamida , Estabilidade Enzimática , Concentração de Íons de Hidrogênio , Íons , Cinética , Metais/farmacologia , Dados de Sequência Molecular , Filogenia , Proteínas Recombinantes/isolamento & purificação , Análise de Sequência de DNA , Especificidade por Substrato/efeitos dos fármacos , Temperatura
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