RESUMO
Long intergenic noncoding RNA 01232 (LINC01232) was identified as a critical regulator of the development of pancreatic adenocarcinoma. The present study investigated the expression and regulatory roles of LINC01232 in esophageal squamous cell carcinoma (ESCC). The main aim of the present study was to elucidate the underlying mechanisms through which LINC01232 affects the malignancy of ESCC. Initially, LINC01232 expression in ESCC was analyzed using the TCGA and GTEx databases and was confirmed using reverse transcriptionquantitative polymerase chain reaction. ESCC cell proliferation, apoptosis and migration and invasion were assessed using the Cell Counting kit8 assay, flow cytometric analysis, and migration and invasion assays, respectively. ESCC tumor growth in vivo was examined using a xenograft mouse model. As shown by the results, a high LINC01232 expression was detected in ESCC tissues and cell lines. LINC01232 downregulation suppressed the proliferation, migration and invasion of ESCC cells, and promoted cell apoptosis in vitro. In addition, LINC01232 depletion restricted tumor growth in vivo. Mechanistically, LINC01232 was shown to function as an microRNA6543p (miR6543p) sponge in ESCC cells, and hepatomaderived growth factor (HDGF) was identified as a direct target of miR6543p. LINC01232 could bind competitively to miR6543p and decrease its expression in ESCC cells, thereby promoting HDGF expression. Rescue experiments reconfirmed that the effects of LINC01232 deficiency in ESCC cells were restored by increasing the output of the miR6543p/HDGF axis. On the whole, the present study demonstrates that LINC01232 plays a tumorpromoting role during the progression of ESCC by regulating the miR6543p/HDGF axis. The LINC01232/miR6543p/HDGF pathway may thus provide a novel theoretical basis for the management of ESCC.
Assuntos
Progressão da Doença , Neoplasias Esofágicas/genética , Carcinoma de Células Escamosas do Esôfago/genética , Carcinoma de Células Escamosas do Esôfago/patologia , Regulação Neoplásica da Expressão Gênica , Peptídeos e Proteínas de Sinalização Intercelular/genética , MicroRNAs/metabolismo , RNA Longo não Codificante/genética , Idoso , Animais , Sequência de Bases , Linhagem Celular Tumoral , Proliferação de Células/genética , Neoplasias Esofágicas/patologia , Feminino , Técnicas de Silenciamento de Genes , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Masculino , Camundongos Endogâmicos BALB C , Camundongos Nus , MicroRNAs/genética , Pessoa de Meia-Idade , RNA Longo não Codificante/metabolismo , Regulação para Cima/genéticaRESUMO
By herbalogical study and investigation, "Zhanba" used by Mongolia doctors mainly contains 7 species from 3 genera of 1 family, but "Zhanba" in Inner Mongolia Standard of Medicinal Materials only contains 3 species which are Althaea rosea, Malva sylvestris L. var. mauritiana and M. verticillata.
