[Role of whey protein and whey components in weight management and energy metabolism].

An anti-obesity effect of dietary calcium and dairy foods is evident from animal studies, observational and population studies, and randomized clinical trials. Dietary calcium appears to be responsible for 50% of the anti-obesity bioactivity of dairy. The additional dairy bioactivity has not been fully identified, but is localized primarily in whey, the anti-obesity effect appears to result from calcium, high proportion of branched chain amino acids, and specific bioactive whey-derived peptides. This article presents recent research progress on the role of whey protein and whey components in weight management and energy metabolism.

[Construction of the subtracted cDNA library in hypothalamus of the seasickness adaptive rats].

OBJECTIVE: To construct subtracted cDNA library in hypothalamus of the seasickness adaptive rats for providing theoretical basis for effective adaptive training against seasickness. METHODS: Suppression subtract hybridization technique was used, and forward and reverse hybridization was performed on the hypothalamus of seasickness adaptive rats and that of normal rats so that to construct subtracted cDNA library. Dot blot was used for differential screening the subtracted library. RESULTS: 23 fragments of differentially expressed genes was obtained including 10 up-regulating and 13 down-regulating fragments. CONCLUSION: Many played role in adaptability formation to seasickness such as SAM, vasopressin, and heme oxygenase.

[Effect of apoptosis induced by different vitamin E homologous analogues in human hepatoma cells(HepG2)].

To observe the effect of apoptosis induced by alpha-tocopherol(alpha-T), gamma-tocopherol(gamma-T), delta-tocopherol(delta-T), and vitamin E succinate (VES) in human hepatoma cells (HepG2), apoptosis was detected by flow cytometry (FCM) and DNA ladder with different VE homologues analogues (alpha-T, gamma-T, delta-T and VES) and different concentrations (12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L and 200 mg/L) at 48 h. The growth of HepG2 cells was inhibited by delta-T and VES at 12.5-200 mg/L in comparison with the negative control group, while (-T showed weak effect of inhibition and alpha-T did not show any inhibition effect. However, as an exception, alpha-tocopherol, delta-tocopherol and VES were effective in induction of apoptosis in HepG2 cells at concentrations of 12.5-200 mg/L. gamma-tocopherol showed effect only at 200 mg/L. Conclusions delta-tocopherol and VES lowered HepG2 cells growth and viability, and increased apoptotic propensity significantly. A dose-dependent of antiproliferation and induction apoptosis was found in HepG2 cells line. The order of efficiency of four vitamin E analogues was delta-tocopherol > VES > gamma-tocopherol > alpha-tocopherol. The difference in nature and magnitude of the anticancer effects did not correlate with their reported antioxidant activity and this might be due to minor differences in their structure important to their biological activities. The results from this study suggested that delta-tocopherol and VES could be the promising anti-hepatoma agents.

[Effect of apoptosis induced by different vitamin E homologous analogues in human hepatoma cells (HepG2)].

To observe the effect of alpha-tocopherol(gamma-T), delta-tocopherol(delta-T), delta-tocopherol(delta-T), and vitamin E succinate (VES) on apoptosis induced in human hepatoma cells (HepG2), the cell apoptosis was detected by flow cytometry (FCM) and DNA ladder under the treatment of different VE homologues analogues (alpha-T, gamma-T, delta-T and VES) at different concentrations (12.5, 25, 50, 100 and 200 mg/L) for 48 hours. The results showed that the growth of HepG2 cells was inhibited by delta-T and VES at 12.5-200 mg/L in comparison with the negative control group, while gamma-T showed weak effect of inhibition and alpha-T did not show any inhibition effect. With the exception of alpha-tocopherol, delta-tocopherol and VES were effective in induction of apoptosis in HepG2 cells at concentrations of 12.5-200 mg/L. gamma-tocopherol showed effect only at 200 mg/L. delta-tocopherol and VES decreased HepG2 cells growth and viability, and increased apoptotic propensity significantly. A dose-dependent of antiproliferation and induction of apoptosis were found in HepG2 cells line. The order of efficiency of four vitamin E analogues was delta-tocopherol > VES > gamma-tocopherol > alpha-tocopherol. It is suggested that delta-tocopherol and VES may be a agent that has a anti-hepatomatic potential.

[Effect of different vitamin E homologous analogues on human hepatoma cell HepG2 proliferation in vitro].

To examine the effect of alpha-tocopherol(alpha-T), gamma-tocopherol(gamma-T), delta-tocopherol(delta-T), and vitamin E succinate (VES) on the proliferation of human hepatoma cell (HepG2), cell proliferation was detected by hemocytometer count, MTT assay and flow cytometry (FCM) with different VE homologues analogues (alpha-T, gamma-T, delta-T and VES) and different concentrations (12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L and 200 mg/L). The results showed that The growth of HepG2 cells was inhibited by delta-T and VES at the dosages of 12.5-200 mg/L in comparison with the negative control group, while gamma-T showed weak effect of inhibition and alpha-T did not show any inhibition effect. The dose range to produce inhibition effects varied with different analogues. A dose-dependent inhibition of cell growth was found in HepG2 cell lines treated with different vitamin E homologues analogues. An accumulation of cells in G0/G1-phase and a significant decreasing of cells in S-phase were found as evaluated by flow cytometric analysis employing a PI-staining method. It is suggested that delta-tocopherol and VES decreased HepG2 cells growth and viability. A dose-dependent of anti-proliferation was found in HepG2 cells line. the order of efficiency of four vitamin E analogues was delta-tocopherol > VES > gamma-tocopherol > alpha-tocopherol. The proliferation was blocked in S-phase. The difference in nature and magnitude of the anticancer effects does not correlate with their reported relative antioxidant activity and might be due to minor differences in their structure important to their biological activities. These results suggest that delta-tocopherol and VES could be promising anti-hepatoma agents.