RESUMO
Lens focused proton radiograph on thin objects is demonstrated using an 11-MeV proton cyclotron at China Academy of Engineering Physics. The proton beam exiting from the tested objects is focused onto the image plane by a magnetic lens system mitigating image blur caused by multiple Coulomb scattering. Both simulations and experiments show that clear images can be obtained with a lens system for the objects with thickness up to 2.7 × 10-2 g/cm2 and the error for the areal density measurement is measured to be less than 2.3%. For the objects with thickness between 2.7 × 10-3 g/cm2 and 2.7 × 10-2 g/cm2, 100-200 µm of spatial resolution is achieved.
RESUMO
In the present study, we focused on the expression and biological functions of TMEM45B in pancreatic cancer tissues and cell lines. Real-time PCR and Western blotting were used to examine the expression levels of TMEM45B in pancreatic cancer tissues and cell lines. The functions of TMEM45B were evaluated using CCK-8, flow cytometry and transwell analysis. Our results showed that TMEM45B exhibited high expression in pancreatic cancer tissues and cell lines compared with the normal pancreatic tissues and cells. Using gene set enrichment analysis (GSEA), we found that TMEM45B may regulate multiple genes involved in the cell cycle and metastasis pathways. Downregulation of TMEM45B by RNA interference significantly reduced proliferation, invasion and migration of SW1990 and PANC-1 cells, accompanied by the induction of cell cycle arrest and apoptosis, whereas overexpression of TMEM45B promoted proliferation, invasion and migration of CFPAC-1 cells as well as apoptosis inhibition. Taken together, our study provides evidence that TMEM45B is an oncogene involved in the tumorigenesis of pancreatic cancer and may represent a new molecular target for pancreatic cancer treatment.
