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1.
Hortic Res ; 11(4): uhae040, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38623073

RESUMO

The gaseous plant hormone ethylene regulates plant development, growth, and responses to stress. In particular, ethylene affects tolerance to salinity; however, the underlying mechanisms of ethylene signaling and salt tolerance are not fully understood. Here, we demonstrate that salt stress induces the degradation of the ethylene receptor ETHYLENE RESPONSE 3 (RhETR3) in rose (Rosa hybrid). Furthermore, the TspO/MBR (Tryptophan-rich sensory protein/mitochondrial benzodiazepine receptor) domain-containing membrane protein RhTSPO interacted with RhETR3 to promote its degradation in response to salt stress. Salt tolerance is enhanced in RhETR3-silenced rose plants but decreased in RhTSPO-silenced plants. The improved salt tolerance of RhETR3-silenced rose plants is partly due to the increased expression of ACC SYNTHASE1 (ACS1) and ACS2, which results in an increase in ethylene production, leading to the activation of ETHYLENE RESPONSE FACTOR98 (RhERF98) expression and, ultimately accelerating H2O2 scavenging under salinity conditions. Additionally, overexpression of RhETR3 increased the salt sensitivity of rose plants. Co-overexpression with RhTSPO alleviated this sensitivity. Together, our findings suggest that RhETR3 degradation is a key intersection hub for the ethylene signalling-mediated regulation of salt stress.

2.
ACS Nano ; 17(18): 18470-18480, 2023 09 26.
Artigo em Inglês | MEDLINE | ID: mdl-37669408

RESUMO

Self-assembling virus-like particles (VLPs) can tolerate a wide degree of genetic and chemical manipulation to their capsid protein to display a foreign molecule polyvalently. We previously reported the successful incorporation of foreign peptide sequences in the junction loop and onto the C-terminus of PP7 dimer VLPs, as these regions are accessible for surface display on assembled capsids. Here, we report the implementation of a library-based approach to test the assembly tolerance of PP7 dimer capsid proteins to insertions or terminal extensions of randomized 15-mer peptide sequences. By performing two iterative rounds of assembly-based selection, we evaluated the degree of favorability of all 20 amino acids at each of the 15 randomized positions. Deep sequencing analysis revealed a distinct preference for the inclusion of hydrophilic peptides and negatively charged amino acids (Asp and Glu) and the exclusion of positively charged peptides and bulky and hydrophobic amino acid residues (Trp, Phe, Tyr, and Cys). Within the libraries tested here, we identified 4000 to 22,000 unique 15-mer peptide sequences that can successfully be displayed on the surface of the PP7 dimer capsid. Overall, the use of small initial libraries consisting of no more than a few million members yielded a significantly larger number of unique and assembly-competent VLP sequences than have been previously characterized for this class of nucleoprotein particle.


Assuntos
Aminoácidos , Peptídeos , Sequência de Aminoácidos , Capsídeo , Proteínas do Capsídeo/genética , Polímeros
3.
PLoS One ; 18(8): e0286024, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37531364

RESUMO

Image fusion technology is employed to integrate images collected by utilizing different types of sensors into the same image to generate high-definition images and extract more comprehensive information. However, all available techniques derive the features of the images by utilizing each sensor separately, resulting in poorly correlated image features when different types of sensors are utilized during the fusion process. The fusion strategy to make up for the differences between features alone is an important reason for the poor clarity of fusion results. Therefore, this paper proposes a fusion method via information clustering and image features (ICIF). First, the weighted median filter algorithm is adopted in the spatial domain to realize the clustering of images, which uses the texture features of an infrared image as the weight to influence the clustering results of the visible light image. Then, the image is decomposed into the base layer, bright detail layer, and dark detail layer, which improves the correlations between the layers after conducting the decomposition of a source graph. Finally, the characteristics of the images collected by utilizing sensors and feature information between the image layers are used as the weight reference of the fusion strategy. Hence, the fusion images are reconstructed according to the principle of extended texture details. Experiments on public datasets demonstrate the superiority of the proposed strategy over state-of-the-art methods. The proposed ICIF highlighted targets and abundant details as well. Moreover, we also generalize the proposed ICIF to fuse images with different sensors, e.g., medical images and multi-focus images.


Assuntos
Algoritmos , Luz , Processamento de Imagem Assistida por Computador/métodos
5.
Biomacromolecules ; 24(5): 2003-2008, 2023 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-37126604

RESUMO

The therapeutic value of delivering recombinant uricase to human patients has been appreciated for decades. The development of therapeutic uricases has been hampered by the fact that humans do not encode an endogenous uricase and therefore most recombinant forms of the protein are recognized as foreign by the immune system and are therefore highly immunogenic. In order to both shield and stabilize the active enzyme, we encapsulated a functional ancestral uricase in recombinant, noninfectious Qß capsid nanoparticles and characterized its catalytic activity. Oral delivery of the nanoparticles moderated key symptoms of kidney dysfunction in uricase-knockout mice by lowering uric acid levels. Histological kidney samples of the treated mice suggest that delivery of recombinant uricase had a protective effect against the destructive effects of uric acid that lead to renal failure caused by hyperuricemia.


Assuntos
Hiperuricemia , Nanopartículas , Humanos , Camundongos , Animais , Hiperuricemia/tratamento farmacológico , Hiperuricemia/genética , Ácido Úrico , Urato Oxidase/genética , Camundongos Knockout
6.
PLoS One ; 17(12): e0278055, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36584047

RESUMO

Multi-scale image decomposition is crucial for image fusion, extracting prominent feature textures from infrared and visible light images to obtain clear fused images with more textures. This paper proposes a fusion method of infrared and visible light images based on spatial domain and image features to obtain high-resolution and texture-rich images. First, an efficient hierarchical image clustering algorithm based on superpixel fast pixel clustering directly performs multi-scale decomposition of each source image in the spatial domain and obtains high-frequency, medium-frequency, and low-frequency layers to extract the maximum and minimum values of each source image combined images. Then, using the attribute parameters of each layer as fusion weights, high-definition fusion images are through adaptive feature fusion. Besides, the proposed algorithm performs multi-scale decomposition of the image in the spatial frequency domain to solve the information loss problem caused by the conversion process between the spatial frequency and frequency domains in the traditional extraction of image features in the frequency domain. Eight image quality indicators are compared with other fusion algorithms. Experimental results show that this method outperforms other comparative methods in both subjective and objective measures. Furthermore, the algorithm has high definition and rich textures.


Assuntos
Algoritmos , Processamento de Imagem Assistida por Computador , Processamento de Imagem Assistida por Computador/métodos , Raios Infravermelhos
7.
STAR Protoc ; 3(2): 101428, 2022 06 17.
Artigo em Inglês | MEDLINE | ID: mdl-35664258

RESUMO

Bispecific antibodies are a powerful new class of therapeutics, but their development often requires enormous amounts of time and resources. Here, we describe a high-throughput protocol for cloning, expressing, purifying, and evaluating bispecific antibodies. This protocol enables the rapid screening of large panels of bispecific molecules to identify top candidates for further development. For complete details on the use and execution of this protocol, please refer to Estes et al. (2021).


Assuntos
Anticorpos Biespecíficos , Anticorpos Biespecíficos/uso terapêutico , Clonagem Molecular
8.
BMC Complement Med Ther ; 22(1): 25, 2022 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-35086536

RESUMO

BACKGROUND: Osteoarthritis (OA) treatment aims to improve inflammation and delay cartilage degeneration. However, there is no effective strategy presently available. Ononin, a representative isoflavone glycoside component extracted from natural Chinese herbs, exerts anti-inflammatory and proliferative effects. However, the therapeutic effect of ononin on chondrocyte inflammation remains unclear. METHODS: In this study, we explored the therapeutic effect and potential mechanism of ononin in OA by establishing an interleukin-1 beta (IL-1ß)-induced chondrocyte inflammation model. RESULTS: Our results verified that ononin alleviated the IL-1ß-induced decrease in chondrocyte viability, attenuated the overexpression of the inflammatory factors tumour necrosis factor α (TNF-α) and interleukin 6 (IL-6), and simultaneously inhibited the expression of cartilage extracellular matrix (ECM)-degrading enzymes such as matrix metalloproteinase-13 (MMP-13). Furthermore, the decomposition of Collagen II protein could be alleviated in the OA model by ononin. Finally, ononin improved chondrocyte inflammation by downregulating the mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NF-κB) signalling pathways. CONCLUSION: Our findings suggested that ononin could inhibit the IL-1ß-induced proinflammatory response and ECM degradation in chondrocytes by interfering with the abnormal activation of the MAPK and NF-κB pathways, indicating its protective effect against OA.


Assuntos
Cartilagem/efeitos dos fármacos , Glucosídeos/farmacologia , Inflamação/metabolismo , Interleucina-1beta/metabolismo , Isoflavonas/farmacologia , Proteínas Quinases Ativadas por Mitógeno/metabolismo , NF-kappa B/metabolismo , Osteoartrite , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Cartilagem/citologia , Cartilagem/metabolismo , Cartilagem/patologia , Condrócitos/efeitos dos fármacos , Condrócitos/metabolismo , Condrócitos/patologia , Regulação para Baixo , Glucosídeos/uso terapêutico , Inflamação/tratamento farmacológico , Isoflavonas/uso terapêutico , Sistema de Sinalização das MAP Quinases , Masculino , Metaloproteinase 13 da Matriz/metabolismo , Osteoartrite/tratamento farmacológico , Osteoartrite/metabolismo , Osteoartrite/patologia , Fitoterapia , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Ratos Sprague-Dawley , Transdução de Sinais , Fator de Necrose Tumoral alfa/metabolismo
9.
Sci Rep ; 11(1): 9682, 2021 05 06.
Artigo em Inglês | MEDLINE | ID: mdl-33958613

RESUMO

The need for high-affinity, SARS-CoV-2-specific monoclonal antibodies (mAbs) is critical in the face of the global COVID-19 pandemic, as such reagents can have important diagnostic, research, and therapeutic applications. Of greatest interest is the ~ 300 amino acid receptor binding domain (RBD) within the S1 subunit of the spike protein because of its key interaction with the human angiotensin converting enzyme 2 (hACE2) receptor present on many cell types, especially lung epithelial cells. We report here the development and functional characterization of 29 nM-affinity mouse SARS-CoV-2 mAbs created by an accelerated immunization and hybridoma screening process. Differing functions, including binding of diverse protein epitopes, viral neutralization, impact on RBD-hACE2 binding, and immunohistochemical staining of infected lung tissue, were correlated with variable gene usage and sequence.


Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , COVID-19/imunologia , SARS-CoV-2/imunologia , Animais , COVID-19/diagnóstico , Teste Sorológico para COVID-19 , Epitopos/imunologia , Feminino , Humanos , Imunização , Camundongos , Camundongos Endogâmicos BALB C , Modelos Moleculares , SARS-CoV-2/isolamento & purificação , Glicoproteína da Espícula de Coronavírus/imunologia
10.
Plant Cell ; 33(4): 1229-1251, 2021 05 31.
Artigo em Inglês | MEDLINE | ID: mdl-33693903

RESUMO

Flowers are the core reproductive structures and key distinguishing features of angiosperms. Flower opening to expose stamens and gynoecia is important in cases where pollinators much be attracted to promote cross-pollination, which can enhance reproductive success and species preservation. The floral opening process is accompanied by the coordinated movement of various floral organs, particularly petals. However, the mechanisms underlying petal movement and flower opening are not well understood. Here, we integrated anatomical, physiological, and molecular approaches to determine the petal movement regulatory network using rose (Rosa hybrida) as a model. We found that PETAL MOVEMENT-RELATED PROTEIN1 (RhPMP1), a homeodomain transcription factor (TF) gene, is a direct target of ETHYLENE INSENSITIVE3, a TF that functions downstream of ethylene signaling. RhPMP1 expression was upregulated by ethylene and specifically activated endoreduplication of parenchyma cells on the adaxial side of the petal (ADSP) base by inducing the expression of RhAPC3b, a gene encoding the core subunit of the Anaphase-Promoting Complex. Cell expansion of the parenchyma on the ADSP base was subsequently enhanced, thus resulting in asymmetric growth of the petal base, leading to the typical epinastic movement of petals and flower opening. These findings provide insights into the pathway regulating petal movement and associated flower-opening mechanisms.�.


Assuntos
Etilenos/metabolismo , Flores/crescimento & desenvolvimento , Rosa/crescimento & desenvolvimento , Ciclopropanos/farmacologia , Etilenos/farmacologia , Flores/efeitos dos fármacos , Flores/genética , Flores/metabolismo , Regulação da Expressão Gênica de Plantas , Inativação Gênica , Células Vegetais/fisiologia , Reguladores de Crescimento de Plantas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Rosa/efeitos dos fármacos , Rosa/genética , Rosa/metabolismo
11.
Chem Commun (Camb) ; 57(20): 2531-2534, 2021 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-33564799

RESUMO

CNT/VS2-MoS2 with a multi-interface structure shows significantly enhanced catalytic activity compared with MoS2, CNT/MoS2, CNT/VS2 and VS2-MoS2, including a low overpotential of -215 mV at 10 mA cm-2 and a small Tafel slope of 64 mV dec-1. The enhanced catalytic activity could be ascribed to the multiple interfaces in CNT/VS2-MoS2, which can promote charge transfer and yield abundant active sites. This study provides a valuable route to improve the catalytic performance of two-dimensional electrocatalysts.

12.
PLoS One ; 15(9): e0239341, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32976534

RESUMO

BACKGROUND: It is unclear whether there are individual differences in the long-term efficacy of computer-assisted and traditional total knee arthroplasty. The purpose of this study was to perform a meta-analysis comparing the same individuals undergoing computer-assisted and traditional total knee arthroplasty separately to determine whether computer-assisted total knee arthroplasty can provide better lower extremity radiographic results and clinical outcomes. METHODS: We searched literatures to identify relevant randomized controlled trials comparing the effects of computer-assisted and traditional methods in bilateral total knee arthroplasty. After screening, quality evaluation and data extraction according to inclusion and exclusion criteria, the quality and bias risks of the included studies were evaluated. The meta-analysis compared the radiographic results, functional outcomes and complications of the two techniques. RESULTS: Six clinical controlled trials were included, with total of 1098 patients. The meta-analysis showed that the accuracy in terms of the mechanical axis of the lower extremity, the sagittal alignment of the femoral component and the coronal alignment of the tibial component in computer-assisted total knee arthroplasty was significantly better than those in traditional total knee arthroplasty. There were no differences in the functional results, revision rates or aseptic loosening rates between the two techniques. CONCLUSION: After excluding individual differences such as bone development and bone quality, although computer-assisted techniques can better accurately correct the mechanical axis of the lower extremity and the position of prosthesis implantation than traditional techniques, there is no significant difference in the functional results and revision rate of bilateral total knee arthroplasty in the same individual.


Assuntos
Artroplastia do Joelho/estatística & dados numéricos , Ensaios Clínicos Controlados Aleatórios como Assunto , Cirurgia Assistida por Computador/estatística & dados numéricos , Artroplastia do Joelho/efeitos adversos , Humanos , Cirurgia Assistida por Computador/efeitos adversos , Análise de Sobrevida , Resultado do Tratamento
13.
J Biol Chem ; 295(46): 15438-15453, 2020 11 13.
Artigo em Inglês | MEDLINE | ID: mdl-32883809

RESUMO

Widespread testing for the presence of the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in individuals remains vital for controlling the COVID-19 pandemic prior to the advent of an effective treatment. Challenges in testing can be traced to an initial shortage of supplies, expertise, and/or instrumentation necessary to detect the virus by quantitative RT-PCR (RT-qPCR), the most robust, sensitive, and specific assay currently available. Here we show that academic biochemistry and molecular biology laboratories equipped with appropriate expertise and infrastructure can replicate commercially available SARS-CoV-2 RT-qPCR test kits and backfill pipeline shortages. The Georgia Tech COVID-19 Test Kit Support Group, composed of faculty, staff, and trainees across the biotechnology quad at Georgia Institute of Technology, synthesized multiplexed primers and probes and formulated a master mix composed of enzymes and proteins produced in-house. Our in-house kit compares favorably with a commercial product used for diagnostic testing. We also developed an environmental testing protocol to readily monitor surfaces for the presence of SARS-CoV-2. Our blueprint should be readily reproducible by research teams at other institutions, and our protocols may be modified and adapted to enable SARS-CoV-2 detection in more resource-limited settings.


Assuntos
Teste de Ácido Nucleico para COVID-19/métodos , COVID-19/diagnóstico , Kit de Reagentes para Diagnóstico/economia , SARS-CoV-2/genética , Transferência de Tecnologia , Universidades/economia , Biotecnologia/métodos , COVID-19/virologia , Humanos , Kit de Reagentes para Diagnóstico/provisão & distribuição , Reação em Cadeia da Polimerase em Tempo Real/métodos , SARS-CoV-2/isolamento & purificação
14.
medRxiv ; 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32766604

RESUMO

Widespread testing for the presence of the novel coronavirus SARS-CoV-2 in individuals remains vital for controlling the COVID-19 pandemic prior to the advent of an effective treatment. Challenges in testing can be traced to an initial shortage of supplies, expertise and/or instrumentation necessary to detect the virus by quantitative reverse transcription polymerase chain reaction (RT-qPCR), the most robust, sensitive, and specific assay currently available. Here we show that academic biochemistry and molecular biology laboratories equipped with appropriate expertise and infrastructure can replicate commercially available SARS-CoV-2 RT-qPCR test kits and backfill pipeline shortages. The Georgia Tech COVID-19 Test Kit Support Group, composed of faculty, staff, and trainees across the biotechnology quad at Georgia Institute of Technology, synthesized multiplexed primers and probes and formulated a master mix composed of enzymes and proteins produced in-house. Our in-house kit compares favorably to a commercial product used for diagnostic testing. We also developed an environmental testing protocol to readily monitor surfaces across various campus laboratories for the presence of SARS-CoV-2. Our blueprint should be readily reproducible by research teams at other institutions, and our protocols may be modified and adapted to enable SARS-CoV-2 detection in more resource-limited settings.

15.
Biochemistry ; 59(31): 2870-2881, 2020 08 11.
Artigo em Inglês | MEDLINE | ID: mdl-32786888

RESUMO

The properties of enzymes packaged within the coat protein shell of virus-like particles (VLPs) were studied to provide a comprehensive assessment of such factors. Such entrainment did not seem to perturb enzyme function, but it did significantly enhance enzyme stability against several denaturing stimuli such as heat, organic solvents, and chaotropic agents. This improvement in performance was found to be general and independent of the number of independent subunits required and of the number of catalytically active enzymes packaged. Packaged enzymes were found by measurements of intrinsic tryptophan fluorescence to retain some of their native folded structure even longer than their catalytic activity, suggesting that protein folding is a significant component of the observed catalytic benefits. While we are unable to distinguish between kinetic and thermodynamic effects - including inhibition of enzyme unfolding, acceleration of refolding, and biasing of folding equilibria - VLP packaging appears to represent a useful general strategy for the stabilization of enzymes that operate on diffusible substrates and products.


Assuntos
Enzimas/metabolismo , Leviviridae , Vírion/metabolismo , Biocatálise , Estabilidade Enzimática/efeitos dos fármacos , Enzimas/química , Desdobramento de Proteína/efeitos dos fármacos , Solventes/farmacologia
16.
Biomacromolecules ; 21(6): 2432-2439, 2020 06 08.
Artigo em Inglês | MEDLINE | ID: mdl-32441521

RESUMO

Near-IR fluorescent Qß virus-like particles (VLPs) were produced in a high yield by packaging highly red-shifted monomeric and dimeric versions of biliverdin-dependent fluorescent proteins within the capsid shell. The simple addition of biliverdin hydrochloride to the medium during or after Escherichia coli protein expression was enough to produce fully matured encapsidated fluorophores. The packaged near-IR proteins exhibited identical photochemical properties to their nonencapsidated analogues but were far more stable toward heat, chaotrope-induced denaturation, and proteolysis. Noninvasive in vivo imaging showed the VLPs to traffic primarily to the liver after systemic injection in mice, revealing that the particles were easily detected by a standard instrument.


Assuntos
Proteínas do Capsídeo , Capsídeo , Animais , Escherichia coli , Camundongos
17.
Plant Physiol Biochem ; 151: 69-76, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32200192

RESUMO

The basic region/leucine zipper (bZIP) transcription factors play key roles in regulating diverse biological processes in plants. However, their participation in shoot branching has been rarely reported. Here, we isolated a CmbZIP1 transcription factor gene, a member of the bZIP family, from chrysanthemum. Subcellular localization analysis indicated that CmbZIP1 is a nuclear protein. Tissue-specific expression analysis indicated that CmbZIP1 was principally expressed in apical bud and axillary bud. Expression patterns analysis results showed that CmbZIP1 expression was suppressed in axillary buds in response to decapitation but increased in response to shade. Overexpression of CmbZIP1 in Arabidopsis inhibits its shoot branching. In addition, expression of auxin efflux protein PIN-FORMED 1 (PIN1) and auxin signaling components AUXIN RESISTANT 1/3 (AXR1, AXR3) were significantly up-regulated in overexpressing plants in comparison with wild type plants. Moreover, the transcript expression of BRANCHED 2 (AtBRC2) was also significantly up-regulated in overexpressing plants compared with the wild type. Altogether, these results suggest important and negative roles of CmbZIP1 in shoot branching. Our study extends the understanding of the function of bZIP transcription factors in plants and provides valuable gene resources for improving the architectural traits of ornamental plants.


Assuntos
Fatores de Transcrição de Zíper de Leucina Básica/fisiologia , Chrysanthemum/crescimento & desenvolvimento , Proteínas de Plantas/fisiologia , Brotos de Planta/crescimento & desenvolvimento , Arabidopsis , Regulação da Expressão Gênica de Plantas , Proteínas Nucleares/fisiologia , Plantas Geneticamente Modificadas
18.
ACS Nano ; 13(4): 4443-4454, 2019 04 23.
Artigo em Inglês | MEDLINE | ID: mdl-30912918

RESUMO

As self-assembling polyvalent nanoscale structures that can tolerate substantial genetic and chemical modification, virus-like particles are useful in a variety of fields. Here we describe the genetic modification and structural characterization of the Leviviridae PP7 capsid protein as a platform for the presentation of functional polypeptides. This particle was shown to tolerate the display of sequences from 1 kDa (a cell penetrating peptide) to 14 kDa (the Fc-binding double Z-domain) on its exterior surface as C-terminal genetic fusions to the coat protein. In addition, a dimeric construct allowed the presentation of exogenous loops between capsid monomers and the simultaneous presentation of two different peptides at different positions on the icosahedral structure. The PP7 particle is thereby significantly more tolerant of these types of polypeptide additions than Qß and MS2, the other Leviviridae-derived VLPs in common use.


Assuntos
Proteínas do Capsídeo/química , Capsídeo/química , Leviviridae/química , Biblioteca de Peptídeos , Peptídeos/análise , Sequência de Aminoácidos , Capsídeo/ultraestrutura , Proteínas do Capsídeo/genética , Proteínas do Capsídeo/ultraestrutura , Leviviridae/genética , Leviviridae/ultraestrutura , Modelos Moleculares , Peptídeos/genética , Engenharia de Proteínas
19.
Gene ; 688: 119-131, 2019 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-30529094

RESUMO

The HAP3 subfamily gene RcLEC1-B, was isolated from protocorm-like body (PLB) of Rosa canina, encodes 213 amino acid residues. It was shown that RcLEC1-B was specifically expressed in PLB of R. canina and its subcellular localization is in the nucleus. Overexpression of RcLEC1-B in Arabidopsis resulted in a decrease in endogenous ABA level, an increase in GA, IAA and CTK contents, and an increased number of branches. RcLEC1-B promotes the formation of spontaneous embryoids, suggesting that it may be a homolog of the Arabidopsis LEC1 gene. RcLEC1-B-OE changed the number and morphology of flower organs and resulted in open carpels and exposed ovules, along with a reduced percentage of fertile fruit. This is the first observation that overexpression of a homolog of LEC1 in Arabidopsis can lead to morphological changes in floral organs, cuticle defects, and adhesions between organs; this may result from the increased level of gibberellin in the transgenic plants.


Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Flores/genética , Regulação da Expressão Gênica de Plantas/genética , Giberelinas/genética , Rosa/genética , Fatores de Transcrição/genética , Sequência de Aminoácidos , Sequência de Bases , Núcleo Celular/genética , Regulação da Expressão Gênica no Desenvolvimento/genética , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas/genética , Alinhamento de Sequência
20.
Int J Mol Sci ; 19(6)2018 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-29843424

RESUMO

Single-flower cut Chrysanthemum (Dendranthema grandiflorum 'Jinba') holds a unique status in global floriculture industry. However, the extensive axillary bud outgrowth presents a major drawback. Shade is an environment cue that inhibits shoot branching. Present study was aimed at investigating the effect of ratio of red to far-red light (R:FR) in regulating the lateral bud outgrowth of Chrysanthemum and the detailed mechanism. Results showed that the fate of axillary buds at specific positions in stem exhibited difference in response to R:FR. Decreasing R:FR resulted in elevation of abscisic acid (ABA) accumulation in axillary buds. Expression of ABA, indole-3-acetic acid (IAA) and strigolactones (SL) -related metabolism and signal transduction genes was significantly changed in response to low R:FR. In addition, low R:FR caused the re-distribution of sucrose across the whole plant, driving more sucrose towards bottom buds. Our results indicate that low R:FR not always inhibits bud outgrowth, rather its influence depends on the bud position in the stem. ABA, SL and auxin pathways were involved in the process. Interestingly, sucrose also appears to be involved in the process which is necessary to pay attention in the further studies. The present study also lays the foundation for developing methods to regulate axillary bud outgrowth in Chrysanthemum.


Assuntos
Chrysanthemum/efeitos da radiação , Regulação da Expressão Gênica de Plantas , Reguladores de Crescimento de Plantas/metabolismo , Proteínas de Plantas/genética , Brotos de Planta/efeitos da radiação , Ácido Abscísico/metabolismo , Chrysanthemum/genética , Chrysanthemum/crescimento & desenvolvimento , Chrysanthemum/metabolismo , Jardinagem/métodos , Perfilação da Expressão Gênica , Humanos , Ácidos Indolacéticos/metabolismo , Lactonas/metabolismo , Luz , Proteínas de Plantas/metabolismo , Brotos de Planta/genética , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/metabolismo , Transdução de Sinais , Sacarose/metabolismo
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