Establishment of rat model of silicotuberculosis and its pathological characteristic.

OBJECTIVE: To establish different stages of silicosis rat model complicated with tuberculosis infection, and compare the pathological characteristics and analyze the impact of silicosis on tuberculosis infection. METHODS: SD rats were subjected to intratracheal administration of silica with non-exposure method at the 1st, 30th, or 60th day. At the 50th day, the rats were injected with the suspension of H37Rv (a virulent standard strain of Mycobacterium tuberculosis) via tail-vein. After 40 days post-infection, rats were sacrificed, the lung tissues were isolated, and paraffin was embedded and sectioned. The sections were treated using HE staining for structure observation, acid fast stain of Ziehl-Neelsen for bacterial detection, and Warthin-Starry silver staining for displaying the distribution of dust particles. The bacterial load was quantified by colony counting. RESULTS: Primary to tertiary silicosis could be discovered at the 30th, 60th, and 90th day of post-infection. The rats could be infected by injection of M. tuberculosis via tail vein, with tuberculosis load and the degree of lung tissue lesions positively correlated with silicosis. CONCLUSION: The rat model of silicotuberculosis was established successfully, which facilitated understanding the 'cross-talk' of silicosis and tuberculosis during the process they drive each other.

The research of the correlation of obstructive sleep apnea-hypopnea syndrome and insulin resistance / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate whether obstructive sleep apnea-hypopnea syndrome (OSAHS) is relevant to insulin resistance (IR) and whether it is an independent factor of IR.@*METHOD@#Forty-eight OSAHS patients (OSAHS group) excluding obesity, high blood sugar, high blood fat and hypertension and 45 health adults without OSAHS (control group) were included in this research. The sleeping parameters such as AHI, AI, SIT90, IAT, MSaO2, LSaO2, etc., the blood pressure and abdomen circumference on both groups were measured. Six milliliter blood was drawn to measure blood sugar level and insulin level using enzyme linked immunosorbent assay in spectively.@*RESULT@#There were 9 cases (18.7%) in OSAHS group whose IR > or =5, while there were 6 cases (13.3%) in the contrast group whose IR > or =5. There were no significant differences between the two groups (P > 0.05).@*CONCLUSION@#It could be concluded from this research that OSAHS is not an independent factor of IR, while obesity, high blood sugar, high blood fat, and hypertension, etc, are high relevant to IR.