RESUMO
Silver pomfret (Pampus argenteus) is one of the most important fishery products in the Indo-Pacific Ocean. Nonetheless, little is known about the genetic parameters of economic traits. To determine whether the selective breeding programme would improve economic traits, the authors evaluated the growth performance of the selected group and the control group at 60, 90 and 120 days, and the growth performance parameters of the selected group were higher than those of the control group. Through Pearson correlation analysis, the authors found that the morphological traits that are highly related to the weight of P. argenteus are body length and body height. Genetic gain and realized heritability for body weight were highest at day 90, which were 12.72% and 0.23%, respectively. Therefore, the authors speculate that day 90 may be a critical time for screening. The average coefficient of variation for body weight reached over 30%. All three morphological traits reached moderate realized heritability at day 90 after hatching. These implied that selective breeding has the potential to improve the growth traits of P. argenteus. In addition, the reproductive performance of P. argenteus parent fish was obviously improved after gonadal strengthening. These results provide important information for future breeding programmes for P. argenteus.
Assuntos
Perciformes , Animais , Perciformes/genética , Peixes/genética , Fenótipo , Peso Corporal/genética , Oceano PacíficoRESUMO
Hsp90, a molecular chaperone, was generally thought to be a unique cytoplasmic form in invertebrates, playing important roles in multiple cellular stress responses. Now, two cytoplasmic Hsp90 cDNAs (ptHSP90-1 and ptHSP90-2 genes) were isolated from an invertebrate - crab Portunus trituberculatus. Main features, sequence identities and phylogenetic analysis with other species were described. Expression profiles in tissues and under stressful conditions were analyzed using semi-quantitative RT-PCR method. ptHSP90-1 and ptHSP90-2 were constitutively expressed with higher transcript levels in ovary and muscle, respectively. A cold treatment rapidly activated both ptHSP90s transcription in hepatopancreas and gill, but caused the ptHSP90-2 mRNA decrease in muscle and ovary. Under heat treatment ptHSP90-1 mRNA was accumulated in hepatopancreas and muscle (but down-regulated in ovary), while ptHSP90-2's transcription tendency in each tissue was the same as that in cold shock. Moreover, the transcriptional levels of both ptHSP90 genes under Cu(2+) stress were evaluated. This crab exposed to the low and high salinity exhibited either lower expression levels of both ptHSP90s or no changes in four tissues except the up-regulation of ptHSP90-2 transcription in hepatopancreas. These results suggested there were at least two Hsp90s in P. trituberculatus, which played differing roles in physiological and stressful conditions.