Moderate nitrogen application improved salt tolerance by enhancing photosynthesis, antioxidants, and osmotic adjustment in rapeseed (Brassica napus L.).

Salt stress is a major adverse environmental factor limiting plant growth. Nitrogen (N) application is an effective strategy to alleviate the negative effects of salt stress on plants. To improve the knowledge of the mechanism of N application on alleviating salt stress on rapeseed seedlings, a pot experiment was conducted with four N application treatments (0, 0.1, 0.2, and 0.3 g N kg-1 soil, referred to as N0, N1, N2, and N3, respectively) and exposed to non-salt stress (0 g NaCl kg-1 soil, referred to as S0) and salt stress (3 g NaCl kg-1 soil, referred to as S1) conditions. The results indicated that in comparison with non-salt stress, salt stress increased the Na content (236.53%) and reactive oxygen species (ROS) production such as hydrogen peroxide (H2O2) (30.26%), resulting in cell membrane lipid peroxidation characterized by an increased content of malondialdehyde (MDA) (122.32%) and suppressed photosynthetic rate (15.59%), finally leading to inhibited plant growth such as shorter plant height, thinner root neck, lower leaf area, and decreased dry weight. N application improved the plant growth, and the improvement by N application under salt stress was stronger than that under non-salt stress, suggesting that rapeseed seedlings exposed to salt stress are more sensitive to N application and require N to support their growth. Moreover, seedlings exposed to salt stress under N application showed lower ROS accumulation; increased photosynthesis; higher antioxidants such as catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), and ascorbic acid (AsA); and greater accumulation of osmotic substances including soluble protein, soluble sugar, and proline, as compared with seedlings without N application. In particular, the best improvement by N application under salt stress occurred at the N2 level, while too high N application could weaken the improvement due to inhibited N metabolism. In summary, this study suggests that moderate N application can improve photosynthesis, antioxidants, and osmoregulation to alleviate the adverse effects of salt stress in rapeseed seedlings.

Salt stress decreases seed yield and postpones growth process of canola (Brassica napus L.) by changing nitrogen and carbon characters.

Salt stress is a major challenge for plant growth and yield achievement in canola (Brassica napus L.). Nitrogen (N) is considered as an essential nutrient involved in many physiological processes, and carbon (C) is the most component of plant biomass. N and C assimilations of canola plants are always inhibited by salt stress. However, the knowledge of how salt stress affects biomass and seed yield through changing N and C characters is limited. A field experiment was conducted to investigate the growth process, N and C characters, photosynthetic performance, biomass accumulation and seed yield under the low and high soil salt-ion concentration conditions (LSSC and HSSC). The results indicated that HSSC postponed the time of early flowering stage and maturity stage by 4 ~ 5 days and 6 ~ 8 days, respectively, as compared with LSSC. Besides, HSSC decreased the N and C accumulation and C/N at both growing stages, suggesting that salt stress break the balance between C assimilation and N assimilation, with stronger effect on C assimilation. Although the plant N content under HSSC was increased, the photosynthesis rate at early flowering stage was decreased. The leaf area index at early flowering stage was also reduced. In addition, HSSC decreased N translocation efficiency especially in stem, and N utilization efficiency. These adverse effects of HSSC together resulted in reduced biomass accumulation and seed yield. In conclusion, the high soil salt-ion concentration reduced biomass accumulation and seed yield in canola through changing N and C characters.