Sorafenib extends the lifespan of C. elegans through mitochondrial uncoupling mechanism.

Sorafenib is a targeted anticancer drug in clinic. Low-dose sorafenib has been reported to activate AMPK through inducing mitochondrial uncoupling without detectable toxicities. AMPK activation has been the approach for extending lifespan, therefore, we investigated the effect of sorafenib on lifespan and physical activity of C. elegans and the underlying mechanisms. In the present study, we found that the effect of sorafenib on C. elegans lifespan was typically hermetic. Sorafenib treatment at higher concentrations (100 µM) was toxic but at lower concentrations (1, 2.5, 5 µM) was beneficial to C. elegans. Sorafenib (1 µM) treatment for whole-life period extended C. elegans lifespan and improved C. elegans physical activity as manifested by increasing pharyngeal pumping and body movement, preserving intestinal barrier integrity, muscle fibers organization and mitochondrial morphology. In addition, sorafenib (1 µM) treatment enhanced C. elegans stress resistance. Sorafenib activated AMPK through inducing mitochondrial uncoupling in C. elegans. Sorafenib treatment activated DAF-16, SKN-1, and increased SOD-3, HSP-16.2, GST-4 expression in C. elegans. Sorafenib treatment induced AMPK-dependent autophagy in C. elegans. We conclude that low-dose sorafenib protects C. elegans against aging through activating AMPK/DAF-16 dependent anti-oxidant pathways and stimulating autophagy responses. Low-dose sorafenib could be a strategy for treating aging and aging-related diseases.

Tailoring 3D-printed high internal phase emulsion-rice starch gels: Role of amylose in rheology and bioactive stability.

This study investigated the properties of 3D-printed high internal phase emulsion (HIPE)-rice starch gels, specially tailored for personalized nutrition by co-encapsulating resveratrol and ß-carotene. We examined the influence of amylose content on various parameters, including functional groups, linear and nonlinear rheology, printed precision and microstructural stability. Additionally, we assessed the protective efficacy and release in vitro digestion of these gels on the encapsulated bioactive components. Compared to HIPE, HIPE-starch gels differently impacted by amylose content in starches. Low-level amylose weakened the network structure, attributed to amylose mainly responsible for gel formation and weak hydrogen bond interaction between the surface-active molecules and amylose due to gelatinized starch granules rupturing the protein network. Oppositely, high-level amylose led to denser, more gel-like structures with enhanced mechanical strength and reversible deformation resistance, making them suitable for 3D printing. Furthermore, 3D-printed gels with high-level amylose demonstrated well-defined structures, smooth surfaces, stable printing and less dimension deviation. They were also regarded as effective entrapping and delivery systems for resveratrol and ß-carotene, protecting them against degradation from environment and damage under the erosion of digestive fluid. Overall, this research offers a straightforward strategy for creating reduced-fat HIPE gels that serve as the carrier for personalized nutraceutical foods.

Sources, morphology, phytochemistry, pharmacology of Curcumae Longae Rhizoma, Curcumae Radix, and Curcumae Rhizoma: a review of the literature.

Curcumae Longae Rhizoma (turmeric), Curcumae Radix and Curcumae Rhizoma are derived from the Curcuma species, and have gradually become three of the most commonly used medicinal herbs in China due to their different origins, processing methods and medicinal part. These three herbs have certain similarities in morphology, chemical composition, and pharmacological effects. All three of these herbs contain curcuminoids and volatile oil compounds, which exhibit anti-inflammatory, anti-tumor, antioxidant, and neuroprotective properties, although modern clinical applications have their own requirements. At present, there is no systematic guidelines for the clinical application of these three of Curcuma species; consequently, there is a high risk of unwanted phenomena associated with the mixing and indiscriminate use of these herbs. In this review, we focus predominantly on morphology, chemical composition, and the pharmacological activity of these three Curcuma herbs and summarize the current status of research in this field. Our goal is to provide a better understanding of clinical value of these Curcuma species so that we can provide reference guidelines for their further development, utilization and rational clinical application.