Correlation of lipocalin 2 and glycolipid metabolism and body composition in a large cohort of children with osteogenesis imperfecta.

PURPOSE: Lipocalin 2 (LCN2) is a newly recognized bone-derived factor that is important in regulation of energy metabolism. We investigated the correlation of serum LCN2 levels and glycolipid metabolism, and body composition in a large cohort of patients with osteogenesis imperfecta (OI). METHODS: A total of 204 children with OI and 66 age- and gender-matched healthy children were included. Circulating levels of LCN2 and osteocalcin were measured by enzyme-linked immunosorbent assay. Serum levels of fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TC), and low- and high-density lipoprotein cholesterol (LDL-C, HDL-C) were measured by automated chemical analyzers. The body composition was measured by dual-energy X-ray absorptiometry. Grip strength and timed-up-and-go (TUG) were tested to evaluate the muscle function. RESULTS: Serum LCN2 levels were 37.65 ± 23.48 ng/ml in OI children, which was significantly lower than those in healthy control (69.18 ± 35.43 ng/ml, P < 0.001). Body mass index (BMI) and serum FBG level were significantly higher and HDL-C levels were lower in OI children than healthy control (all P < 0.01). Grip strength was significantly lower (P < 0.05), and the TUG was significantly longer in OI patients than healthy control (P < 0.05). Serum LCN2 level was negatively correlated to BMI, FBG, HOMA-IR, HOMA-ß, total body, and trunk fat mass percentage, and positively correlated to total body and appendicular lean mass percentage (all P < 0.05). CONCLUSIONS: Insulin resistance, hyperglycemia, obesity, and muscle dysfunction are common in OI patients. As a novel osteogenic cytokine, LCN2 deficiency may be relevant to disorders of glucose and lipid metabolism, and dysfunction of muscle in OI patients.

Effects of Acute Alcohol Consumption on the Human Brain: Diffusional Kurtosis Imaging and Arterial Spin-Labeling Study.

BACKGROUND AND PURPOSE: Brain function and microstructure are affected by alcohol consumption. Until recently, the effect of alcohol on neural mechanisms has not been fully elucidated. Our aim was to explore the acute effects of alcohol on healthy human brains by diffusional kurtosis imaging and 3D arterial spin-labeling and elucidate structural and functional changes in the brain on acute alcohol intake. MATERIALS AND METHODS: Conventional MR imaging, diffusional kurtosis imaging, and 3D arterial spin-labeling were performed on 24 healthy volunteers before and 0.5 and 1 hour after drinking alcohol. Participants were divided into 2 groups according to the response to alcohol: blushing (n = 12) and unblushing (n = 12) groups. Twenty brain regions were analyzed. RESULTS: Diffusional kurtosis imaging revealed an increase in mean kurtosis and fractional anisotropy at 0.5 hour post-alcohol intake in most brain regions, whereas mean diffusion was decreased in several brain regions at 1 hour after drinking. 3D arterial spin-labeling showed increased cerebral blood flow in most brain regions, particularly in the frontal regions. However, perfusion in the anterior commissure decreased. Regional changes in the brain correlated with various behavioral performances with respect to blush response and sex. In general, blushing individuals and men are more sensitive to alcohol with acute effects. CONCLUSIONS: Physiologic and microstructural alterations in the brain on alcohol consumption were examined. Brain areas with blood flow alteration detected by 3D arterial spin-labeling were highly consistent with susceptible areas detected by diffusional kurtosis imaging. The current study provides new insight into the effects of alcohol on the brain and behavioral performance in different blush response and sex populations.

Direct tuberculosis drug susceptibility testing: time-saving and cost-effective in detecting MDR-TB.

BACKGROUND: Recent advances have made molecular diagnosis of tuberculosis (TB) and drug susceptibility testing (DST) possible, but the high costs involved present a huge challenge. The refinement and improvement of affordable methods therefore remain a priority. Conventional indirect DST is inexpensive and reliable, but time-consuming. A direct DST method for the direct testing of sputum samples without culture has been developed to reduce the time required for DST, but there have been conflicting results. METHODS AND RESULTS: Direct and indirect DST against isoniazid and rifampicin were performed on 208 sputum smear-positive specimens, 186 from newly diagnosed patients and 22 from previously treated patients; respectively 169 and 180 of the direct and indirect DST results were reportable. In comparison with indirect DST, direct DST resulted in a saving of on average 10.5 days. The time to direct DST results was inversely correlated with the number of acid-fast bacilli in the sputum samples. CONCLUSION: Direct DST is highly sensitive, reliable, cost-effective and time-saving in comparison with indirect DST.

Chemokine C-C motif ligand 18 expression correlates with tumor malignancy in breast cancer.

PURPOSE OF THE STUDY: To investigate whether CCL18 is involved in breast cancer, and the relationship between CCL18 and MVD (MVD was recognized by CD34) which is a well-accepted angiogenic maker of multiple cancers including breast cancer. PATIENTS AND METHODS: Immunohistochemistry staining for CCL18 and CD34 was performed on 179 cases, including 29 normal cases as control, 47 cases with benign breast diseases, and 103 cases with breast cancer. RESULTS: We found that CCL18 was significantly up-regulated in breast cancer samples as compared with benign tumors or normal breast tissues. Moreover, the expression level of CCL18 increased with the size of tumors, the number of lymph node metastasis, and advancing tumor stage, suggesting that CCL18 expression correlates with tumor malignancy scales. At the same time, we found that MVD was also significantly over-expressed in cancer tissues as compared with normal control group and benign tumor group, but it was not significantly differentially expressed among tumors with different malignancy scale like CCL18, while the expression of MVD in CCL18 positive breast cancer cases was higher than in the CCL18 negative breast cancer cases (P=0.016, P<0.05). CONCLUSION: CCL18 is involved in the development of breast cancer. CCL18 is a better biomarker than MVD in determining whether the tumor is malignant and the severity of malignancy of breast cancer.

Effects of broccoli stem and leaf meal on broiler performance, skin pigmentation, antioxidant function, and meat quality.

Three hundred sixty 1-d-old Ross 308 male broilers were used to study the effects of broccoli stem and leaf meal (BSLM) on growth performance, skin pigmentation, antioxidant function, and meat quality. The chicks were fed 4 diets containing different levels (0, 4.0, 8.0, and 12.0%) of BSLM as partial replacement for corn and soybean meal for a period of 42 d. The results showed that dietary supplementation of BSLM had no effect (P > 0.05) on growth performance. As compared with control, dietary 4%, 8%, and 12% BSLM increased (P < 0.05) b value (yellowness) both in shank and breast skin, increased (P < 0.05) the concentrations of xanthophylls in abdominal fat and breast skin, improved (P < 0.05) total antioxidant capability, lowered malondialdehyde concentration, and decreased drip loss percentage of breast muscle. Dietary 8% and 12% BSLM decreased (P < 0.05) shank L values (lightness), increased (P < 0.05) shank a value (redness), and increased (P < 0.05) the activities of superoxide dismutase and catalase of breast muscle as compared with control. The results indicated that dietary supplementation of BSLM in broiler chickens improved the poultry products quality with the more skin pigmentation and the less drip loss percentage of breast meat. The more skin pigmentation mainly related to the high amount of xanthophylls in BSLM. The decreased meat drip loss fed BSLM may be caused by the antioxidative function of BSLM.

Acute effects of alcohol on the human brain: diffusion tensor imaging study.

BACKGROUND AND PURPOSE: DTI can provide information about brain ultrastructure by quantifying water diffusion. Our objective was to assess the value of DTI in detecting the acute effects of alcohol on healthy human brains. MATERIALS AND METHODS: Sixteen healthy volunteers were studied with conventional MR imaging and DTI before and 0.5, 1, 2, and 3 hours after the initiation of acute alcohol administration. Two DTI parameters, FA and ADC, were measured in the frontal lobe, internal capsule, external capsule, precentral gyrus, postcentral gyrus, thalamus, middle cerebellar peduncle, and brain stem. BrACs were measured at each time point after drinking to estimate BACs. RESULTS: No abnormalities were found by conventional MR imaging at any time point in all subjects. ADC values of the frontal lobe, thalamus, and middle cerebellar peduncle were significantly reduced, reaching a minimum value at 1 or 2 hours, and FA values of the frontal lobe were significantly increased, reaching a maximal value at 0.5 hour in both doses. BrAC (BAC) was significantly increased to reach a peak at 0.5 hour in both doses and decreased gradually. CONCLUSIONS: DTI can detect changes in brains after acute alcohol consumption that are not detectable by conventional MR imaging. The frontal lobe, thalamus, and middle cerebellar peduncle are more vulnerable to the effects of acute alcohol consumption. DTI is more effective than BrAC or BAC for the detection of alcohol-induced changes on the human brain.

Prediction of recovery from a post-traumatic coma state by diffusion-weighted imaging (DWI) in patients with diffuse axonal injury.

INTRODUCTION: To determine whether diffusion-weighted magnetic resonance (MR) imaging findings combined with initial clinical factors indicate the depth of shearing lesions in the brain structure and therefore relate to coma duration in diffuse axonal injury (DAI). METHODS: A total of 74 adult patients (48 male and 26 female) with DAI were examined with conventional MR imaging and diffusion-weighted MR imaging between 2 hours and 20 days after injury. Apparent diffusion coefficient (ADC) maps were obtained and the mean ADC values of each region of interest (ROI) were measured using MRI console software. The involvement of the brainstem, deep gray matter, and corpus callosum was determined for each sequence separately as well as for the combination of all sequences. The correlations between MR imaging findings indicating the presence of apparent brain injury combined with initial clinical factors were determined. RESULTS: Clinical characteristics, such as initial score on the Glasgow coma scale (GCS), age and number of all lesions, and ADC scores were predictive of the duration of coma. CONCLUSION: It was possible to predict post-traumatic coma duration in DAI from cerebral MR imaging findings combined with clinical prognostic factors in the acute to subacute stage after head injury. Age, ADC scores, GCS score and number of lesions were highly significant in predicting coma duration. The technique presented here might provide a tool for in vivo detection of DAI to allow the prediction of the coma duration during the early stages in patients with traumatic brain injury.

Coma duration prediction in diffuse axonal injury: analyses of apparent diffusion coefficient and clinical prognostic factors.

The purpose of this paper is to evaluate the hypothesis that the Apparent Diffusion Coefficient (ADC) values combined with initial clinical factors indicates the depth of shearing lesions in the brain structure and therefore relates to coma duration of diffuse axonal injury (DAI). Seventy-four adult patients (48 male and 26 female patients) with diffuse axonal injury were examined with convention MR imaging and diffusion weighted MR imaging between 2 hours and 20 days after injury. Apparent diffusion coefficient (ADC) maps were obtained and the mean ADC values of each Region of Interest (ROI) were measured using MRI console software. The lesions involvement of brainstem, deep gray matter, and corpus callosum were determined for each sequence separately as well as for the combination of all sequences. The correlations between magnetic resonance (MR) imaging findings of presence of apparent brain injury combined with initial clinical factors were investigated. Clinical characteristics, such as initial score on the Glasgow Coma Scale (GCS), age, and the number of all lesions, ADC scores of the patient in MR findings were predictive of the duration of coma. Post-traumatic coma duration of DAI could be predicted by cerebral MRI findings in the acute to subacute stage after head injury combined with clinical prognostic factors. Age, ADC scores, GCS, number of lesions are highly significant in predicting coma duration. The technique presented herein might provide a tool for in vivo detection of DAI for the coma duration at the early stages in patients with traumatic brain injury.

Functional magnetic resonance mapping of motor cortex in patients with mass lesions near primary motor and sensory cortices.

PURPOSE: To study motor cortex mapping in patients with mass lesions near primary motor and sensory cortices with BOLD-fMRI. MATERIAL AND METHODS: 18 patients with mass lesions near primary motor and sensory cortices, and 8 healthy volunteers were investigated with fMRI using a 1.5 T GE Signa scanner. The specific task was repetitive selfpaced index finger to thumb opposition with a frequency of approximately 2 Hz. Each task paradigm consisted of twelve 20-second blocks alternating between rest and activation. All functional data was sent to SUN GE Advanced Workstation 4.0 for post processing. RESULTS: 14 patients showed functional activation near mass lesion, 4 patients failed to show, and one patient with meningeoma was excluded because of heavy head movement. Of 14 patients, the functional activation of eloquent cortex was different between the group with declination of muscle force and the group with normal muscle force, generally the activated areas of the former were more scattered, dislocated, relative smaller than that of the latter. CONCLUSION: fMRI is a valuable method for pre-operative evaluation of neurosurgical patients and probably can evaluate the muscle force pre- and post-operation approximately.