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1.
Food Funct ; 11(5): 4707-4718, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32409814

RESUMO

Ferulic acid (FA) has been shown to have a neuroprotective effect on Alzheimer's disease induced by amyloid-beta (Aß) neurotoxicity. This work aims to ascertain the structure-activity relationship of FA and its alkyl esters (FAEs) for evaluating the antioxidant activities in PC12 cells and Aß1-42 aggregation inhibitory activities in vitro, as well as the signaling mechanisms against oxidative stress elicited by Aß1-42 in PC12 cells. Our data showed that alterations in the subcellular localization and cytotoxicity of FAEs caused by the lipophilicity of FA were crucial when evaluating their antioxidant capacities. Pre-treating cells with butyl ferulate (FAC4) significantly attenuated Aß1-42-evoked intracellular ROS formation. Besides, FAC4 exhibited the highest Aß1-42 aggregation inhibitory effectiveness. The molecular docking results showed that FAC4 binds to amide NH in Gln15 and Lys16 via a hydrogen bond. Notably, FAC4 could upregulate antioxidant defense systems by modulating the Keap1-Nrf2-ARE signaling pathway. Identification of the functions of FAEs could be useful in developing food supplements or drugs for treating AD.


Assuntos
Doença de Alzheimer/tratamento farmacológico , Peptídeos beta-Amiloides/efeitos dos fármacos , Ácidos Cumáricos/uso terapêutico , Fármacos Neuroprotetores/uso terapêutico , Peptídeos beta-Amiloides/metabolismo , Animais , Ácidos Cumáricos/administração & dosagem , Ácidos Cumáricos/farmacologia , Humanos , Simulação de Acoplamento Molecular , Fator 2 Relacionado a NF-E2/metabolismo , Fármacos Neuroprotetores/administração & dosagem , Fármacos Neuroprotetores/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Células PC12/efeitos dos fármacos , Células PC12/metabolismo , Ratos
2.
Mol Med Rep ; 16(4): 4008-4014, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28765922

RESUMO

Electric field (EF) exposure can affect the elongation, migration, orientation, and division of cells. The present study tested the hypothesis that EF may also affect epithelial­mesenchymal transition (EMT) in lens epithelial cells and that this effect may be an important inducer in the pathological process of posterior capsule opacification (PCO). Human lens epithelial (HLE)­B3 cells were exposed to an EF. Experiments were performed in the presence or absence of an anti­integrin ß1 blocking antibody or a small molecule inhibitor targeting focal adhesion kinase (FAK). Cell morphology changes were observed by microscopy. The expression levels of integrin ß1, FAK, phosphorylated (p)FAK and of EMT markers, E­cadherin and Vimentin, were examined by immunofluorescence, reverse transcription­quantitative polymerase chain reaction and western blotting. Following exposure to EF, HLE­B3 cells appeared elongated and resembled more fibroblast­like cells. Expression of E­cadherin was decreased, while expression of Vimentin was increased in HLE­B3 cells exposed to EF, compared with control cells. In addition, the mRNA expression levels of integrin ß1 were increased, and the protein expression levels of integrin ß1 and pFAK were increased in HLE­B3 cells exposed to EF, compared with control cells. Blocking of integrin ß1 suppressed the EMT­related morphological changes of HLE­B3 cells and reduced the activation of FAK following EF exposure. However, blocking of pFAK did not affect the EMT status of HLE­B3 cells induced by EF. In conclusion, the present study demonstrated that EF exposure induced EMT in HLE­B3 cells and that this effect may partially be mediated by the activation of integrin ß1­FAK signaling. The present results may provide a new mechanistic approach to prevent the development of PCO.


Assuntos
Células Epiteliais/metabolismo , Células Epiteliais/efeitos da radiação , Transição Epitelial-Mesenquimal/efeitos da radiação , Proteína-Tirosina Quinases de Adesão Focal/metabolismo , Integrina beta1/metabolismo , Cristalino/citologia , Transdução de Sinais , Biomarcadores , Caderinas/metabolismo , Linhagem Celular , Células Epiteliais/patologia , Transição Epitelial-Mesenquimal/genética , Proteína-Tirosina Quinases de Adesão Focal/genética , Expressão Gênica , Humanos , Integrina beta1/genética , Vimentina/metabolismo
3.
Chin Med J (Engl) ; 130(12): 1481-1490, 2017 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-28584213

RESUMO

BACKGROUND: The E-26 transformation-specific related gene (ERG) is frequently expressed in cytogenetically normal acute myeloid leukemia (CN-AML). Herein, we performed a meta-analysis to investigate the relationship between the prognostic significance of ERG expression and CN-AML. METHODS: A systematic review of PubMed database and other search engines were used to identify the studies between January 2005 and November 2016. A total of 667 CN-AML patients were collected from seven published studies. Of the 667 patients underwent intensive chemotherapy, 429 had low expression of ERG and 238 had high expression of ERG. Summary odds ratio (OR) and the 95% confidence interval (CI) for the ERG expression and CN-AML were calculated using fixed- or random-effects models. Heterogeneity was assessed using Chi-squared-based Q- statistic test and I2 statistics. All statistical analyses were performed using R.3.3.1 software packages (R Foundation for Statistical Computing, Vienna, Austria) and RevMan5.3 (Cochrane Collaboration, Copenhagen, Denmark). RESULTS: Overall, patients with high ERG expression had a worse relapse (OR = 2.5127, 95% CI: 1.5177-4.1601, P = 0.0003) and lower complete remission (OR = 0. 3495, 95% CI: 0.2418-0.5051, P< 0.0001). With regard to the known molecular markers, both internal tandem duplications of the fms-related tyrosine kinase 3 gene (OR = 3.8634, 95% CI: 1.8285-8.1626, P = 0.004) and brain and acute leukemia, cytoplasmic (OR = 3.1538, 95% CI: 2.0537-4.8432, P< 0.0001) were associated with the ERG expression. In addition, the results showed a statistical significance between French-American-British (FAB) classification subtype (minimally differentiated AML and AML without maturation, OR = 4.7902, 95% CI: 2.7772-8.2624, P< 0.0001; acute monocytic leukemia, OR = 0.2324, 95% CI: 0.0899-0.6006, P = 0.0026) and ERG expression. CONCLUSION: High ERG expression might be used as a strong adverse prognostic factor in CN-AML.


Assuntos
Biomarcadores Tumorais/metabolismo , Leucemia Mieloide Aguda/metabolismo , Leucemia Mieloide Aguda/patologia , Perfilação da Expressão Gênica , Humanos , Prognóstico , Regulador Transcricional ERG/metabolismo
4.
Gastric Cancer ; 18(4): 796-802, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25182956

RESUMO

BACKGROUND AND AIMS: Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumor of the digestive tract and characterized by expression of KIT protein. Imatinib is the frontline therapy for metastatic and unresectable GIST patients showing clinical responses in 80 % of cases. Despite the often long-lasting clinical benefit seen in most patients treated with imatinib, many will eventually suffer disease progression. The most frequent mechanism of imatinib resistance in GIST is the acquisition of secondary mutations in either KIT or PDGFRA. There are also some imatinib-resistant GIST patients lacking an identifiable mechanism of treatment failure. Recently, activating BRAF mutation was detected in a small percentage of GISTs. In this study, we report a case of GIST with acquired resistance to imatinib during therapy. METHODS: Histological, immunohistochemical, Western blot and mutational analyses were performed on GIST tissues before and after imatinib resistance. RESULTS: The imatinib-resistant tumor showed not only heterogeneous mutations of KIT and BRAF besides the primary mutation, but also transdifferentiation into a rhabdomyosarcoma phenotype. According to Western blot analysis, in imatinib-resistant GIST with both KIT V559D and BRAF V600E mutations, the inhibition of KIT V559D by imatinib caused a strong decrease of AKT phosphorylation, while ERK1/2 phosphorylation was not affected. CONCLUSIONS: This finding, in combination with the loss of KIT expression, suggests the possibility of activation of RAS-RAF-MEK-ERK pathways driven by a KIT-independent oncogenic mechanism. Understanding the genetic aberrations beyond KIT and PDGFRA may lead to the identification of additional therapeutic targets for GISTs.


Assuntos
Transdiferenciação Celular/genética , Resistencia a Medicamentos Antineoplásicos/genética , Tumores do Estroma Gastrointestinal/genética , Mutação , Proteínas Proto-Oncogênicas B-raf/genética , Proteínas Proto-Oncogênicas c-kit/genética , Neoplasias Gástricas/genética , Idoso , Antineoplásicos/uso terapêutico , Western Blotting , Cromatografia Líquida de Alta Pressão , Análise Mutacional de DNA , Tumores do Estroma Gastrointestinal/tratamento farmacológico , Tumores do Estroma Gastrointestinal/patologia , Humanos , Mesilato de Imatinib/uso terapêutico , Imuno-Histoquímica , Masculino , Recidiva Local de Neoplasia/genética , Recidiva Local de Neoplasia/patologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/patologia
5.
Environ Toxicol Pharmacol ; 37(2): 718-28, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24607686

RESUMO

BACKGROUND AND AIMS: The nephrotoxic mechanisms of andrographolide sodium bisulfate (ASB) remain largely unknown. This study attempted to explore the mechanism of ASB-induced nephrotoxicity using human proximal tubular endothelial cells (HK-2). METHODS: For this study HK-2 cells were treated with rising concentrations of ASB. Their survival rate was detected using MTT assay and ultrastructure was observed with electron microscopy. L-Lactate dehydrogenase (LDH) assay was followed by examination of mitochondrial membrane potential (MMP). Reactive oxygen species (ROS) was detected using different methods and apoptosis/autophage related proteins were detected using immunoblotting. RESULTS: We found that ASB inhibited HK-2 cell proliferation and decreased cell survival rate in a time and dose-dependent manner (P<0.05, P<0.01, respectively). With increasing ASB concentration, cell structure was variably damaged and evidence of apoptosis and autophagy were observed. MMP gradually decreased and ROS was induced. The expression of JNK and Beclin-1 increased and activation of the JNK signaling pathway were seen. Apoptosis was induced via the mitochondrial-dependent caspase-3 and caspase-9 pathway, and autophagy related protein Beclin-1 was enhanced by ASB. CONCLUSION: The data show that ASB induces high levels of ROS generation in HK-2 cells and activates JNK signaling. Furthermore, ASB induces cell apoptosis via the caspase-dependent mitochondrial pathway, and induces cellular autophagy, in part by enhancing Beclin-1 protein expression.


Assuntos
Diterpenos/toxicidade , Células Endoteliais/efeitos dos fármacos , Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Sulfatos/toxicidade , Apoptose/efeitos dos fármacos , Proteínas Reguladoras de Apoptose/metabolismo , Autofagia/efeitos dos fármacos , Proteína Beclina-1 , Caspase 3/metabolismo , Caspase 9/metabolismo , Linhagem Celular , Citocromos c/metabolismo , Células Endoteliais/metabolismo , Glutationa/metabolismo , Humanos , Túbulos Renais Proximais/citologia , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Proteínas de Membrana/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Superóxido Dismutase/metabolismo , Proteína X Associada a bcl-2/metabolismo
6.
J Fluoresc ; 22(5): 1395-406, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22733190

RESUMO

Three novel transition metal complexes (Hapy)(2)[M(DCA)(2)]·6H(2)O (M = Mn(II) (1), Ni(II) (2), Cu(II) (3); DCA = demethylcantharate, 7-oxabicyclo[2.2.1]heptane-2,3-dicarboxylate, C(8)H(8)O(5); Hapy = 2-aminopyridine acid, C(5)H(7)N(2)) were synthesized and characterized by elemental analysis, infrared spectra, thermogravimetric analysis and X-ray diffraction. DNA binding properties of the complexes were investigated by electronic absorption spectra, fluorescence spectra and viscosity measurements. Results indicated the complexes could bind to DNA through partial intercalation mode with binding constants K ( b )/(L·mol(-1)) of 1.91 × 10(4) (1), 5.13 × 10(4) (2) and 1.12 × 10(5) (3) at 298 K. Meanwhile, the interactions of the complexes with BSA were also studied by fluorescence spectra. The results suggested that the complexes could quench the fluorescence of BSA through static quenching with the binding constants K ( A )/(L·mol(-1)) of 1.44 × 10(6) (1), 1.14 × 10(7) (2) and 2.98 × 10(4) (3). And the main contribution was tryptophan residues of BSA. The antiproliferative activity test revealed that complexes showed more intense inhibition ratios against human hepatoma cells lines and human gastric cancer cells lines in vitro. Copper(II) complex (3) possesses the strongest inhibition ratio against human hepatoma cells.


Assuntos
Aminopiridinas/química , Antineoplásicos/metabolismo , Antineoplásicos/farmacologia , Cantaridina/análogos & derivados , Cantaridina/química , Compostos Organometálicos/metabolismo , Compostos Organometálicos/farmacologia , Soroalbumina Bovina/metabolismo , Antineoplásicos/química , Sítios de Ligação , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Cobre/química , Cristalografia por Raios X , Humanos , Manganês/química , Níquel/química , Compostos Organometálicos/química , Ligação Proteica , Análise Espectral , Viscosidade
7.
Neurosci Bull ; 28(1): 61-8, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22233890

RESUMO

OBJECTIVE: To determine whether aquaporin-4 (AQP4) regulates acute lesions, delayed lesions, and the associated microglial activation after cryoinjury to the brain. METHODS: Brain cryoinjury was applied to AQP4 knockout (KO) and wild-type mice. At 24 h and on days 7 and 14 after cryoinjury, lesion volume, neuronal loss, and densities of microglia and astrocytes were determined, and their changes were compared between AQP4 KO and wild-type mice. RESULTS: Lesion volume and neuronal loss in AQP4 KO mice were milder at 24 h following cryoinjury, but worsened on days 7 and 14, compared to those in wild-type mice. Besides, microglial density increased more, and astrocyte proliferation and glial scar formation were attenuated on days 7 and 14 in AQP4 KO mice. CONCLUSION: AQP4 deficiency ameliorates acute lesions, but worsens delayed lesions, perhaps due to the microgliosis in the late phase.


Assuntos
Aquaporina 4/fisiologia , Lesões Encefálicas/patologia , Gliose/patologia , Microglia/patologia , Animais , Aquaporina 4/deficiência , Aquaporina 4/genética , Astrócitos/metabolismo , Encéfalo/metabolismo , Modelos Animais de Doenças , Congelamento , Camundongos , Camundongos Knockout , Microglia/metabolismo
8.
Acta Pharmacol Sin ; 31(7): 849-54, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20581858

RESUMO

AIM: To investigate the mechanism of chlorogenic acid (CA)-induced anaphylactoid reactions. METHODS: Degranulation of peritoneal mast cells was assayed by using alcian blue staining in guinea pigs, and the degranulation index (DI) was calculated. CA-induced degranulation of RBL-2H3 cells was also observed and assayed using light microscopy, transmission electron microscopy, flow cytometry, and beta-hexosaminidase release. RESULTS: CA 0.2, 1.0, and 5.0 mmol/L was able to promote degranulation of peritoneal mast cells in guinea pigs in vitro, but it did not increase the degranulation of peritoneal mast cells in CA-sensitized guinea pigs compared with control (P>0.05). Treatment with CA 0.2, 1.0, and 5.0 mmol/L for 30, 60, and 120 min induced degranulation in RBL-2H3 cells in a dose- and time-dependent manner (P<0.01). Under transmission electron microscope typical characteristics of degranulation, including migration of granular vesicles toward the plasma membrane and integration combined with exocytosis, were observed, after CA or C48/80 treatment. Fluorescent microscopy and flow cytometric analysis showed that CA induced concentration-dependent translocation of phosphatidylserine in RBL-2H3 cells. beta-hexosaminidase release in RBL-2H3 cells was significantly increased after incubation with 1 mmol/L CA for 60 min and 5 mmol/L CA for 30 min (P<0.01). CONCLUSION: CA induces degranulation of peritoneal mast cells and RBL-2H3 cells in guinea pigs, which might be one of the mechanisms of the generation of anaphylactoid reactions induced by CA.


Assuntos
Anafilaxia/induzido quimicamente , Degranulação Celular/efeitos dos fármacos , Ácido Clorogênico/toxicidade , Animais , Linhagem Celular Tumoral , Ácido Clorogênico/administração & dosagem , Relação Dose-Resposta a Droga , Citometria de Fluxo , Cobaias , Masculino , Mastócitos/metabolismo , Mastócitos/fisiologia , Microscopia Eletrônica de Transmissão , Peritônio/citologia , Peritônio/metabolismo , Ratos , Fatores de Tempo , beta-N-Acetil-Hexosaminidases/metabolismo
9.
J Fluoresc ; 19(5): 857-66, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19396530

RESUMO

Three novel complexes [Nd(L)(NO3)(H2O)2].NO(3).2H2O (HL1 = N-pyrimidine norcantharidin acylamide acid, C12H13N3O4; HL2 = N-pyridine norcantharidin acylamide acid, C13H14N2O4; HL3 = N-phenyl norcantharidin acylamide acid, C14H15NO4) were synthesized. HL1, HL2 and HL3 are the ligand of complex(1), complex(2) and complex(3), respectively. Their structures were characterized by elemental analysis, conductivity measurement, infrared spectra and thermogravimetric analysis. The DNA-binding properties of the complexes have been investigated by fluorescence spectroscopy and viscosity measurements. The results suggest that the complexes can bind to DNA by partial intercalation. The liner Stern-Volmer quenching constant Ksq values are 3.3(+/-0.21)(1), 1.7(+/-0.19)(2) and 0.9(+/-0.04)(3), respectively. Complex (1) and (2) have been found to cleave pBR322 plasmid DNA at physiological pH and temperature. The test of antiproliferation activity indicates that complex(1) has strong antiproliferative ability against the SMMC7721 (IC50 = 131.7 +/- 23.4 micromol x L(-1)) and A549 (IC50 = 128.4 +/- 19.9 micromol x L(-1)) cell lines. The inhibition rates of complex(2) (IC50 = 86.3 +/- 11.3 micromol x L(-1)) are much higher than that of NCTD (IC50 = 115.5 +/- 9.5 micromol x L(-1)) and HL2 (111.0 +/- 5.7 micromol x L(-1)) against SMMC7721 cell lines.


Assuntos
Amidas/química , Compostos Bicíclicos Heterocíclicos com Pontes/química , DNA/química , Neodímio/química , Compostos Organometálicos/síntese química , Compostos Organometálicos/farmacologia , Animais , Bovinos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Concentração de Íons de Hidrogênio , Ligantes , Compostos Organometálicos/química , Temperatura
10.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 36(2): 123-8, 2007 03.
Artigo em Chinês | MEDLINE | ID: mdl-17443897

RESUMO

OBJECTIVE: To determine whether cysteinyl leukotriene receptor agonist LTD(4) and cysteinyl leukotriene receptor 1 (CysLT(1)) antagonist pranlukast affect the differentiation of human neuroblastoma SK-N-SH cells. METHODS: SK-N-SH cell morphological changes induced by LTD(4), pranlukast and LTD(4) + pranlukast were observed with retinoid acid (RA) as the positive control. The expressions of CysLT(1) and CysLT(2) receptors were detected by immunoblotting analysis, and the expression of microtubule-associated protein-2 (MAP-2), a neuron marker, was detected by fluorescent immunostaining. RESULT: The immunoblotting results showed that SK-N-SH cells expressed CysLT(1) receptor moderately, and CysLT(2) receptor highly. The morphological results showed that RA, pranlukast and LTD(4) + pranlukast induced the compaction of the cell bodies and the outgrowth of neurites, while LTD(4) had no significant effect. The immunostaining results showed that MAP-2 was distributed in the cell bodies in control or pranlukast-treated cells; it was distributed in cell bodies and neuritis in RA-treated cells. Pranlukast increased the numbers of MAP-2-positive cells. CONCLUSION: The CysLT(1)receptor antagonist pranlukast modulates the differentiation of SK-N-SH cells.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Cromonas/farmacologia , Antagonistas de Leucotrienos/farmacologia , Proteínas de Membrana/metabolismo , Receptores de Leucotrienos/metabolismo , Linhagem Celular Tumoral , Humanos , Immunoblotting , Imuno-Histoquímica , Leucotrieno D4/farmacologia , Proteínas Associadas aos Microtúbulos/metabolismo , Neuroblastoma/metabolismo , Neuroblastoma/patologia
11.
Acta Pharmacol Sin ; 25(8): 1090-5, 2004 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-15301745

RESUMO

AIM: To investigate the effect of astilbic acid (3beta, 6beta-dihydroxyolean-12-en-27-oic acid, AA) on human colorectal carcinoma COLO 205 cell proliferation and apoptosis. METHODS: Proliferation of COLO 205 cells was measured by MTT assay. Content of DNA in COLO 205 cell was measured by modified diphenylamine assay. AA-induced morphological changes was observed with fluorescence microscope and transmission electron microscope. DNA fragmentation was visualized by agarose gel electrophoresis. Apoptosis rate and cell cycle distribution were determined by flow cytometric analysis. Expressions of Bcl-2 and Bax proteins were visioned by immunohistochemical analysis. The change of relative mitochondrial transmembrane potential (MTP) in COLO 205 cell was analyzed with FCM after rhodamine 123 staining. RESULTS: The IC50 (96 h) of AA for inhibiting COLO 205 cell proliferation was 61.56+/-0.34 micromol/L. AA induced a marked concentration- and time-dependent inhibition of COLO 205 cell proliferation and reduced the DNA content in COLO 205 cell. Cells treated with AA 64 micromol/L showed typical morphological changes of apoptosis and DNA ladder pattern. The cell cycle was arrested in G0/G1 phase, and the apoptosis rate was 28.25 % for COLO 205 cells treated with AA 64 micromol/L for 48 h. Meanwhile the expression of Bcl-2 protein was decreased while that of Bax was increased and relative MTP was decreased as well. DEVD-CHO 1 micromol/L could increase the viability of COLO 205 cells treated with AA for 48 h. CONCLUSION: AA showed potent inhibitory activity on COLO 205 cells proliferation, and could induce COLO 205 cells apoptosis through disturbing DNA replication, down-regulating Bcl-2 expression, and up-regulating Bax expression, lowering relative MTP, and activating caspase-3 pathway.


Assuntos
Apoptose/efeitos dos fármacos , Caspases/metabolismo , Neoplasias Colorretais/patologia , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/farmacologia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Caspase 3 , Inibidores de Caspase , Divisão Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Neoplasias Colorretais/metabolismo , Fragmentação do DNA , Humanos , Estrutura Molecular , Ácido Oleanólico/química , Ácido Oleanólico/isolamento & purificação , Oligopeptídeos/farmacologia , Plantas Medicinais/química , Rizoma/química , Saxifragaceae/química , Proteína X Associada a bcl-2
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