Synovial GATA1 mediates rheumatoid arthritis progression via transcriptional activation of NOS2 signaling.

Transcriptional regulation of inducible nitric oxide synthase (iNOS) is critically involved in the pathogenesis and progression of rheumatoid arthritis (RA); however, the specific transcription factors that control this process remain largely unidentified. In the present study, it was discovered that expression of the key erythroid factor, globin transcription factor 1 (GATA1), is significantly greater in human RA synovial tissues than in osteoarthritis (OA) tissues. IL 6 was found to induce synovial GATA1 expression in a signal transducer and activator of transcription 3-dependent manner. Functionally, knockdown of GATA1 expression using specific small interfering RNA treatment was found to compromise immunoreaction-elicited expression of proinflammatory cytokines and thus impair invasiveness of the human fibroblast-like synovial cell line MH7A, whereas introduction of exogenous GATA1 was found to promote production of proinflammatory cytokines, leading to greater aggressiveness of MH7A cells. Mechanistically, GATA1 acts as the transcriptional coactivator of NOS2 (the gene encoding iNOS) transcription. Collectively, these data suggest that synovial GATA1 is an essential contributor to development and exacerbation of RA, presumably by inducing NOS2 transcription.

Effect of perioperative modified rehabilitation training exercise in patients undergoing permanent pace-maker implantation and prognosis analysis / 心血管康复医学杂志

Objective:To explore effect of perioperative modified rehabilitation training exercise in patients undergo-ing permanent pacemaker implantation and their prognosis.Methods:A total of 123 patients undergoing permanent pacemaker implantation were enrolled.According their hospitalization time,they were randomly divided into reha-bilitation training group(n=63)and modified rehabilitation training group(n=60).Postoperative 4d incision pain score, training time standard-reaching rate and action specification within one week after surgery were compared between two groups.On third month after surgery,incidence rate of surgical side shoulder pain was followed up,DASH simplified sco-ring table was used to assess daily living capacity and patient satisfaction etc.,and results were statistically analyzed.Re-sults:After permanent pacemaker implantation,compared with rehabilitation training group,there were significant reduc-tions in incidence rates of venous thrombosis(6.35% vs.0%),surgical shoulder pain(17.5% vs.5.0%)and DASH score [(17.05 ± 4.48)scores vs.(14.02 ± 3.85)scores,P<0.05 or <0.01],and significant rise in patient satisfaction(85.7%vs.96.6%),training time standard-reaching rate(28.5% vs.76.7%)and action specification rate(33.3% vs.68.3%) in modified rehabilitation training group,P<0.05 or <0.01. Conclusion:Perioperative modified rehabilitation training exercise can increase effect for rehabilitation training,reduce incidence of complications and improve daily living capacity in patients undergoing permanent pacemaker implantation.