[Harmonic imaging analysis for assessment of morphological changes in mini-pig alveolar bone by normal and increased functional load].

The aim of the study was to reveal the mastication forces effect on the microstructure of mandible bone tissue of mini-pigs by Fouirier harmonic imaging analysis of bone sections images of back scattered electrons and assessment of calcium and phosphorous distribution maps obtained by roentgenofluorescence technique. The results showed that by higher functional loads not only the total content of mineral elements in the bone matrix increased but also the of the low-frequency harmonics in the image spectrum indicating structural heterogeneity decrease in bone mineralization.

[Use of Fourier analysis in hystomorphometry].

The mandible alveolar bone tissue of mini-pig was evaluated by means of radiospectral analysis. Fourier analysis of mineral content distribution in bone slices showed that loss of masticatory load leads to focal demineralization. With time the demineralization foci increased in amount, not in size.

[Comparative study of injuring action of the preparations used for chemical-mechanic removal of carious dentine].

The destruction of human serum albumin was studied under the influence of the preparations used for chemical-mechanic removal of carious dentine (GK-101 and Caricleans, gel #2) and model systems containing sodium hypochlorite and amino compounds. Control contained hydroxide and sodium chloride but not hypochlorite. The degree of albumin destruction was determined by the amount of low molecular weight fragments precipitated by phosphotungstic acid but not by trichloracetic acid. From the results of the study the conclusion was made that in order to reduce the injuring action of hypochlorite upon dental tissues and soft tissues of oral cavity it is more preferable to use systems containing the addition of neutral and basic amino compounds.

[Method for determination of human individual sensitivity to fluoride].

Study for determination of human individual sensitivity to fluoride was performed on 140 patients of CRIS clinics of the age from 20 to 65 years. It was established that even in cases of F-concentrations analogous to high physiological F-concentrations in blood serum (0.19 ppm) in 11.4% of the examined patients the signs of higher F-sensitivity were observed. Human individual sensitivity to fluoride was dependant upon the severity of patient's status. The results received can be used as a base for tactics development of timely and adequate correction of F-dosage.

[Study of protective effect of perftoran on a skin flaps]. / Izuchenie protektornogo deistviia perftorana na kozhnyi loskut.

Differences in the levels of tissue respiration in the rat skin flaps incubated in perfluothane (PF) and normal saline (NS) are regarded as an evidence of local PF effect on tissue substrate. The complex of metabolic changes in the skin fragments under the effect of PF in comparison with skin fragments incubated in NS indicate a protective effect of PF on tissue substrata. Decreased content of malonic dialdehyde and a slower accumulation of tissue liquid in skin flaps incubated in PF, in comparison with the fragments incubated in NS, is worthy of note. It indicates that incubation in PF (in comparison with incubation in NS) is less harmful for cell membranes and induces lesser disorders in water-salt metabolism and edema which are usually associated with tissue lesions developing under conditions of trophic deficiency.

[The effect of immobilized salivary alkaline phosphatase on remineralization processes]. / Vliianie immobilizovannoi shchelochnoi fosfatazy sliuny na protsessy remineralizatsii.

Relationship between alkaline phosphatase immobilized on apatite and remineralization processes has been studied. The enzyme notably accelerated the remineralization. The course of enzymatic remineralization depended on the source of phosphorus (inorganic phosphorus or beta-glycerophosphate). Calcium incorporation in remineralizing surface was the most rapid in the presence of beta-glycerophosphate. This may be explained by a higher rate of formation of apatite under the effect of immobilized alkaline phosphatase, than of brushite or vitlokite.

[The possible mechanisms of alkaline phosphatase and alpha-amylase immobilization in dental enamel]. / Vozmozhnye mekhanizmy immobilizatsii shchelochnoi fosfatazy i al'fa-amilazy na émali zubov.

The kinetic method revealed that by the rate of adsorption on apatite, serum alkaline phosphatase (AlP) is homologous and salivary AlP consists of two fractions: "slow" with the constant of adsorption rate approximating that of serum AlP and "fast" with 5-6 times greater constant. A mechanism of phosphatase immobilization on apatite by two-stage sequential reaction is proposed. The constants of rates of both stages for the serum phosphatase and fast salivary fraction are determined. Difference of the products of both stages by the Michaelis constant (KM) is demonstrated for the fast fraction. The KM of the second-stage immobilization product is close to that of AlP immobilized on dental enamel, which confirms the hypothesis about their identity. In contrast to AlP, both serum and salivary alpha-amylase react with apatite at the same rate and, probably, by the same mechanism as the bulk of salivary and serum protein.