Can online interview efficiently recruit graduate students during COVID-19 pandemic?

China is facing its largest COVID-19 outbreaks since the early days of the pandemic. This makes on-site interview impossible for graduate student admission. How to do online interview efficiently in recruiting graduate students is a question. Here I sort out the experience and feelings.

Polypropylene microplastics affect the physiology in Drosophila model.

Microplastics (MPs) pollution has been a hot research topic in recent years. MPs are ubiquitous throughout the ecological environment and are eventually accumulated in organisms through inhalation or ingestion. However, given that MPs are inert pollutants, their effects on organisms are not clear. In previous study, we have investigated the effects of polyethylene terephthalate MPs on physiology of Drosophila. What is the effect of polypropylene microplastics (PP-MPs)? The results of our experiments show that being exposed to high concentration of PP-MPs have significant effect on Drosophila. PP-MPs exposure can significantly increase locomotor activity and shorten the time of group sleep in Drosophila. In the presence of high concentrations of PP-MPs, the triglyceride content was reduced in females and their ability of egg production was affected. However, there was no significant effect on the level of protein and carbohydrate, or on the food intake. Our experimental results can provide some preliminary data for assessing the potential hazard of PP-MPs to other organisms.

Chronic Consumption of Trehalose Reduces Lifespan in Drosophila Model.

Trehalose has been widely used as a kind of food additives. But in recent years, with several new studies of trehalose, some harmful effects had also been found. Drosophila melanogaster was used as a model organism to explore whether trehalose affects the lifespan. The results showed that high concentrations of trehalose could significantly shorten the lifespan of female flies by 12.5%, when compared to controls.

Senolytic Drug Quercetin Rescues Survival from Blue Light Toxicity in Drosophila Model.

With the increasing frequency of human exposure to blue light, the harmfulness of blue light has received wider attention. The damaging effect of blue light is complex and long-lasting. In this study, Drosophila melanogaster was used as a model organism to investigate the protective effect of the senolytic drug quercetin on blue light toxicity. As one of the first senolytic drugs discovered, quercetin not only has antioxidant properties, but also has been used to treat various neurological disorders. Our study shows that quercetin can effectively prolong the survival of flies under blue light irradiation, and it significantly increases the egg production of female flies under blue light. In addition, after flies intaking quercetin under blue light, both the spontaneous activity and nutrient metabolism show significant sex-specificity. The experimental results provide a potentially effective intervention method for organisms to defend against blue light toxicity, and reveal a new function of the senolytic drug quercetin from another perspective.

Intake of polyamide microplastics affects the behavior and metabolism of Drosophila.

In this study, the effects of polyamide (PA) microplastics on Drosophila were studied by analysing their effects on feeding, fecundity, metabolism and spontaneous activity, using Drosophila as a model organism. In the 0.1 g/L and 1 g/L groups, there was an increase in the amount of food consumed by female Drosophila melanogaster and a decrease in the amount of food consumed by males in both the 0.1 g/L and 20 g/L groups. In the TG assay, males showed a significant decrease in the 10 g/L and 20 g/L groups. The male group showed a significant decrease in protein content in the 10 g/L and 20 g/L groups. Glucose content decreased in the female Drosophila 1g/L and 10 g/L groups. The highest concentration group of 20 g/L showed a decrease in glucose content in male Drosophila. Only the male Drosophila in the highest concentration group showed increased daytime activity. Egg production by females decreased significantly after feeding microplastic food. The above assays demonstrate the potential effects of PA microplastics on flies, where the consumption of food containing microplastics leads to oxidative stress and inflammation, thus affecting the nutritional metabolism of flies.

Luteolin alleviated damage caused by blue light to Drosophila.

Short-wavelength blue light is commonly found in daily life and is harmful to health. In this experiment, we investigated the effect of luteolin on the survival time of Drosophila under the blue light condition of 3000 Lux using Drosophila as the model organism. The results showed that luteolin alleviated the damage suffered by Drosophila under blue light irradiation, significantly prolonged the survival time of Drosophila, prolonged the survival time of male Drosophila in the heat stress assay, increased the activity of female Drosophila in the spontaneous activity assay, and increased the egg production of female Drosophila at the highest concentration, and there was no significant difference in the food intake experiment. We suggest that the increase in survival time of Drosophila under blue light conditions is due to the function of luteolin in resisting oxidative stress.

Differential analysis of negative geotaxis climbing trajectories in Drosophila under different conditions.

The decline of Drosophila climbing behavior is one of the common phenomena of Drosophila aging. The so-called negative geotaxis refers to the natural upward climbing behavior of Drosophila melanogaster after it oscillates to the bottom of the test tube. The strength of climbing ability is regarded as the index of aging change of D. melanogaster. At present, many laboratories use the percentage of 10 fruit flies climbing a specific height in 5 s as a general indicator of the climbing ability of fruit flies. This group research index ignores the climbing performance of a single fruit fly, and the climbing height belongs to the concept of vertical distance in physics, which cannot truly and effectively reflect the concept of curve distance in the actual climbing process of fruit flies. Therefore, based on the image processing algorithm, we added an experimental method to draw the climbing trajectory of a single fruit fly. By comparing the differences in climbing behavior of fruit flies under different sex, group or single, oscillation condition or rotation inversion condition, we can find that the K-Nearest Neighbor target detection algorithm has good applicability in fruit fly climbing experiment, and the climbing ability of fruit flies decreases with age. Under the same experimental conditions, the climbing ability of female fruit flies was greater than that of male fruit flies. The climbing track length of a single fruit fly can better reflect the climbing process of a fruit fly.

Dietary Phytochemicals that Can Extend Longevity by Regulation of Metabolism.

Diet provides energy and nutrition for human survival, and also provides various joy of taste. Extensive studies have shown that the major components of diet, such as protein, carbohydrate and fat, play important roles in regulating aging and longevity. Whether other dietary ingredients can help prevent aging and extend longevity is a very interesting question. Here based on recent findings, we discussed dietary plant ingredients that can extend longevity by regulation of metabolism, targeting TRP channels, mitophagy, senescence pathways and circadian rhythms. Better understanding of the detailed effects and mechanisms of dietary ingredients on longevity regulation, would be helpful for developing new intervention tools for preventing aging and aging related diseases.

Challenges for graduate students' mentors during COVID-19 pandemic.

The outbreak of COVID-19 pandemic results in the suspension of universities' academic laboratory activities. This brought a difficult time for graduate students majored in molecular biology and their mentors, since the student's thesis progress relies on the data from wet experiments in the lab. Even facing the challenges during the pandemic, mentors could still play an active role. This COVID-19 crisis requires mentors to have more thinking, innovation, and sharing in guiding graduate students.

Effects of PET microplastics on the physiology of Drosophila.

Microplastics, as a new type of pollution, have attracted global attention and have become a research focus in recent years. Given the small size of microplastics, they can be ingested by many organisms. In addition, microplastics can enter the human body through the food chain. So, the potential dangers of microplastics can't be ignored. This study took Drosophila as a model organism to delve the physiological effects of polyethylene terephthalate microplastics (PET-MPs). Here, we reported that the higher concentration of PET-MPs was, the more obvious the effect became. The amount of oviposition decreased in female flies exposed, indicating that microplastics affected reproduction. PET-MPs caused the decrease of triglyceride and glucose content in male flies, as well as the decrease of starvation resistance, suggesting the effect of microplastics on energy metabolism. In addition, the 24-h spontaneous activity of flies exposed to PET-MPs increased significantly. The experimental results can help understand the potential impact of microplastics on physiology.