LncRNA HMGA1P4 promotes cisplatin-resistance in gastric cancer.

OBJECTIVE: Long non-coding RNA (lncRNA) HMGA1P4 has been previously reported to be upregulated in gastric cancer (GC). This study aims to investigate the role of HMGA1P4 in cisplatin (DDP)-resistant GC. PATIENTS AND METHODS: HMGA1P4 levels in DDP-resistant GC tissues and cells were determined. Regulatory effects of HMGA1P4 on proliferative and apoptotic abilities in DDP-resistant GC cells and their parental cells were assessed. At last, expression levels of genes associated with multidrug-resistance (MDR) (MDR1, MRP1, mTOR and HIF-1α) and apoptosis (Bax, Bcl-2 and Caspase3) were determined in DDP-resistant GC cells. RESULTS: Results revealed that HMGA1P4 was upregulated in DDP-resistant GC tissues and cells. Overexpression of HMGA1P4 stimulated proliferative rate and suppressed apoptosis in both DDP-resistant GC cells and their parental cells. Moreover, in DDP-resistant GC cells, overexpression of HMGA1P4 upregulated MDR-related genes and downregulated apoptosis-related genes. CONCLUSIONS: HMGA1P4 is upregulated in DDP-resistant GC tissues and cells, and triggers the progression of DDP-resistance in GC.

[Application of a modified tympanic membrane surgical knife with suction and tube device in myringotomy with ventilation tube placement].

Objective:To explore the efficacy of a modified tympanic membrane surgical knife with suction and tube device in myringotomy with ventilation tube placement for the treatment of secretory otitis media.Method:From June of 2014 to December of 2015, 87 cases of secretory otitis media were randomly divided into two groups: One group was treated by general approach to achieve tympanic membrane tube insertion, and another group with modified method. The total effective rate，the rate of tube detachment at 3 months postoperatively, the rate of scar formation or tympanic membrane atrophy, the operation time and the success rate of tube insertion for the first time in two groups were analyzed retrospectively. Result:There was no significantly difference between two groups about the total effective rate，the rate of tube detachment and the rate of scar formation or tympanic membrane atrophy（P>0.05）.However, the duration of operation in general method groupï¼»(11.4±4.3 min）］was significantly longer than that in modified method groupï¼»(8.1±3.6)minï¼½(t=5.412，P<0.05）.In addition, the success rate of tube insertion in general group（81.2%） was significantly lower than that in modified group（93.7%）(χ²=5.397，P<0.05）. Conclusion:The modified method contributed to shorten the duration of operation, improved the success rate of tube insertion and avoided the injury of tympanic membrane and external auditory canal caused by repeated operation.

[The clinical efficacy analysis of the surgery of the posterior approach parotid gland region resection preserving retinal ganglion and parotid fascia].

Objective:To explore the clinical efficacy analysis the surgery of the posterior approach parotid gland resection preserving retinal ganglion and parotid fascia for the treatment of benign parotid tumour.Method:One hundred and twelve cases were randomly divided into control group and treatment group. The control group was treated by anterior approach parotid gland region resection preserving retinal ganglion and parotid fascia. The treatment group was treated by posterior approach parotid gland region resection preserving retinal ganglion and parotid fascia. The data of operation time, postoperative local numbness, Frey syndrome, facial paralysis and postoperative recurrence case were analyzed. Result:There was no postoperative recurrence case in both two groups, and there was no significant difference between two the groups in operation time and the percent of facial paralysis（all P>0.05）.However, the percent of postoperative local numbness and Frey syndrome in control group were significantly higher than those in treatment group（all P<0.05）. Conclusion:The treatment group had similar clinical efficacy with the traditional surgical operation. In addition, the operation of treatment group contributed to decreased postoperative complications.

Clinical application of three-dimensional reconstruction and rapid prototyping technology of multislice spiral computed tomography angiography for the repair of ventricular septal defect of tetralogy of Fallot.

Three-dimensional (3D) reconstruction and rapid prototyping technology (RPT) of multislice spiral computed tomography angiography (CTA) was applied to prepare physical models of the heart and ventricular septal defects of tetralogy of Fallot (ToF) patients in order to explore their applications in the diagnosis and treatment of this complex heart disease. CTA data of 35 ToF patients were collected to prepare l:l 3D solid models using digital 3D reconstruction and RPT, and the resultant models were used intraoperatively as reference. The operations of all 35 patients were completed under the guidance of the 3D solid model, without difficulty. Intraoperative findings of the patients were consistent with the morphological and size changes of the 3D solid model, and no significant differences were found between the patches obtained from the 3D solid model and the actual intraoperative measurements (t = 0.83, P = 0.412). 3D reconstruction and RPT of multislice spiral CTA can accurately and intuitively reflect the anatomy of ventricular septal defects in ToF patients, providing the foundation for a solid model of the complex congenital heart.

[Classification and surgical treatment of lumbosacral nerve root anomalies].

In this paper 12 cases of lumbosacral nerve-root anomalies proved on operations were reported. Two roots with a common conjoined origin entering the common intervertebral foramen separately were seen in two cases; two roots arising respectively from the lateral and ventral sides of the dural sac at the level of upper margin of the pedicle, in which one ran transversely into the foramen and another obliquely ran downward, in one case; two roots with closely adjacent origins, in which one ran transversely into the foramen at the level of upper margin of the pedicle and another obliquely ran downward, in four cases; a single root with abnormally large whereas the adjacent root with abnormally small, in five cases. We proposed a classification method for the sake of appropriate surgical program, which assorted the anomalies into three types: (I) General volume of nerve roots in the spinal canal or nerve-root canal abnormally increased, either the diameter of a single root increases or two roots entered the common. foramen; (II) The origin and course of two roots were anomalous; (III) Combination of I and II.

[Treatment of femoral trochanteric fracture with intramedullary interlocking nailing: a report of 32 cases].

We improved and made the intramedullary interlocking nail and it's instrument for surgical operation in December 1989 by consulting the reported material of the gamma locking nail. 32 cases of the femoral trochanteric fracture were treated by using this fixation system. The results were satisfactory. The patients could stand up and move about beginning from 6th to 13th day after operation. No mechanical failure of implant, infection and other complications occurred in our series. 29 cases were followed-up for 5 to 19 months and showed all of the fractures were healed. Their hip functions were completely or basically recovered. Coxa vara deformity occurred in two cases and one had a 2.5 cm shortening of the lower limb. We consider that the intramedullary interlocking nail conforms to the principle of human biomechanics and it may be used as an effective surgical treatment with the fracture fixation of superior strength and stability for every type of femoral trochanteric fractures.