An Aggregation-Induced Emission-Based Dual Emitting Nanoprobe for Detecting Intracellular pH and Unravelling Metabolic Variations in Differentiating Lymphocytes.

Monitoring T lymphocyte differentiation is essential for understanding T cell fate regulation and advancing adoptive T cell immunotherapy. However, current biomarker analysis methods necessitate cell lysis, leading to source depletion. Intracellular pH (pHi) can be affected by the presence of lactic acid (LA), a metabolic mediator of T cell activity such as glycolysis during T cell activation; therefore, it is a potentially a good biomarker of T cell state. In this work, a dual emitting enhancement-based nanoprobe, namely, AIEgen@F127-AptCD8, was developed to accurately detect the pHi of T cells to "read" the T cell differentiation process. The nanocore of this probe comprises a pair of AIE dyes, TPE-AMC (pH-sensitive moiety) and TPE-TCF, that form a donor-acceptor pair for sensitive detection of pHi by dual emitting enhancement analysis. The nanoprobe exhibits a distinctly sensitive narrow range of pHi values (from 6.0 to 7.4) that can precisely distinguish the differentiated lymphocytes from naïve ones based on their distinct pHi profiles. Activated CD8+ T cells demonstrate lower pHi (6.49 ± 0.09) than the naïve cells (7.26 ± 0.11); Jurkat cells exhibit lower pHi (6.43 ± 0.06) compared to that of nonactivated ones (7.29 ± 0.09) on 7 days post-activation. The glycolytic product profiles in T cells strongly correlate with their pHi profiles, ascertaining the reliability of probing pHi for predicting T cell states. The specificity and dynamic detection capabilities of this nanoprobe make it a promising tool for indirectly and noninvasively monitoring T cell activation and differentiation states.

Effect of Experiment Warming on Soil Fungi Community of Medicago sativa, Elymus nutans and Hordeum vulgare in Tibet.

The uncertainty response of soil fungi community to climate warming in alpine agroecosystems will limit our ability to fully exploit and utilize soil fungi resources, especially in alpine regions. In this study, a warming experiment was conducted in one perennial leguminous agroecosystem [i.e., alfalfa (Medicago sativa)], perennial gramineous agroecosystem (i.e., Elymus nutans) and annual gramineous agroecosystem [i.e., highland barley (Hordeum vulgare L)] in Tibet since 2016 to investigate the response of soil fungi community to climate warming. Soils at two layers (i.e., 0-10 cm and 10-20 cm) were collected in August 2017 to estimate soil fungi community based on the ITS method. The α-diversity, community composition and functional group abundance of soil fungi in the leguminous agroecosystem were more sensitive to climate warming. The α-diversity of soil fungi in the perennial gramineous agroecosystem were more sensitive to climate warming, but topology parameters of soil fungi species cooccurrence network in the annual gramineous agroecosystem were more sensitive to climate warming. Compared with 0-10 cm, soil fungal α-diversity, community composition and functional group abundance at 10-20 cm were more sensitive to climate warming. The topological parameters of soil fungi species cooccurrence network at 0-10 cm in the gramineous agroecosystem were more sensitive to climate warming, but those at 10-20 cm in the leguminous agroecosystem were more sensitive to climate warming. Warming increased the differences of soil fungi α-diversity and functional composition. For the Medicago sativa agroecosystem, warming increased the abundance of soil pathogenic fungi but decreased the abundance of soil symbiotic and saprophytic fungi at 10-20 cm. Therefore, responses of the soil fungi community to climate warming varied with agroecosystem types and soil depth. Climate warming can alter the differences of the soil fungi community among agroecosystems. Changes in soil fungi community caused by climate warming may be detrimental to the growth of alpine crops, at least for perennial Medicago sativa in Tibet.

Creb2 involved in innate immunity by activating PpMitf-mediated melanogenesis in Pteria penguin.

cAMP response element binding protein 2 (CREB2) acts as an intracellular transcriptional factor and regulates many physiological processes, including melanogenesis and melanocyte differentiation. In our previous research, the Creb2 gene has been characterized from Pteria penguin (P. penguin), but its role and regulatory mechanism in P. penguin are still unclear. In this study, first, the function of PpCreb2 in melanogenesis and innate immunity were identified. PpCreb2 silencing significantly decreased the tyrosinase activity and melanin content, indicating PpCreb2 played an important role in melanogenesis. Meanwhile, PpCreb2 silencing visibly suppressed the antibacterial activity of hemolymph supernatant, indicating that PpCreb2 was involved in innate immunity of P. penguin. Second, the PpCreb2 was confirmed to perform immune function by regulating the melanogenesis. The decreased melanin oxidation product due to PpCreb2 silencing triggered the declining of antibacterial activity of hemolymph supernatant, which then could be rescued by adding exogenous melanin oxidation products. Third, the regulation pathway of PpCreb2 involved in innate immunity was analyzed. The promoter sequence analysis of PpMitf discovered 5 conserved cAMP response element (CRE), which were specifically recognized by basic Leucine zipper domain (bZIP) of upstream activation transcription factor. The luciferase activities analysis showed that PpCreb2 could activate the CRE in PpMitf promoter via highly conserved bZIP domain and regulate the expression of PpMitf, which further regulated the PpTyr expression. Therefore, the results collectively demonstrated that PpCreb2 participated in innate immunity by activating PpMitf-mediated melanogenesis in P. penguin.

TLR4 involved in immune response against Vibrio Parahaemolyticus by MyD88-dependent pathway in Crassostrea hongkongensis.

Vibrio parahaemolyticus (V. parahaemolyticus) is a salt-loving gram-negative bacterium, and is the leading cause of mortality in cultured shellfish in recent years. Toll-like Receptor 4 (TLR4) is a classical pattern recognition receptor (PRRs) that recognizes pathogen-associated molecular patterns (PAMPs) of pathogenic microorganism and activates the immune response. However, the function and signal pathway of TLR4 in oyster are still unknown. In this study, a new TLR4 gene was identified from the Crassostrea hongkongensis (C. hongkongensis). The ChTLR4 contained an open reading frame of 2643 bp, encoding 880 amino acids with seven leucine-rich repeat (LRR) domains and a Toll/IL-1R (TIR) domain. The ChTLR4 shared the highest sequence identity (83.0%) with TLR4 of Crassostrea gigas. Tissue expression analysis revealed that ChTLR4 showed the highest constitutive expression in the gill and hepatopancreas, and was significantly upregulated in immune tissues post V. parahaemolyticus infection, especially in gill and hemocytes. Moreover, TLR4 silencing significantly inhibited the immune-enzyme activities, including SOD, CAT, ACP, AKP in gill and LZM in hemolymph supernatant, and increased MDA content in hemolymph supernatant. Meanwhile, the antimicrobial activities of the hemolymph supernatant were also significantly inhibited by TLR4 silencing. These data demonstrated that the ChTLR4 involved in innate immune response of C. hongkongensis against V. parahaemolyticus challenge. Finally, qRT-PCR analysis showed that ChTLR4 silencing clearly inhibited the expression of genes in TLR4-MyD88 pathway, indicating that MyD88-dependent pathway played a crucial role in ChTLR4-mediated immune response against V. parahaemolyticus.

Doping Compensation Enables High-Detectivity Infrared Organic Photodiodes for Image Sensing.

Infrared organic photodiodes have gained increasing attention due to their great application potentials in night vision, optical communication, and all-weather imaging. However, the commonly occurring high dark current and low detectivity impede infrared photodetectors from portable applications at room temperature. Herein, an efficient and generic doping compensation strategy is developed to improve the detectivity of infrared organic photodiodes. A series of n-type organic semiconductors is investigated, and it is found that doping compensation strategy not only reduces the trap density of states and dark currents, but also restrains the nonradiative recombination with improved charge transport and collection. As a result, an ultralow noise spectral density of 8 × 10-15 A Hz-1/2 as well as a high specific detectivity over 1013 Jones in 780-1070 nm is achieved at room temperature. More importantly, the high-performance infrared organic photodiodes can be successfully applied in high-pixel-density image arrays without patterning sensing layers. These findings provide important compensation design insights that will be crucial to further improve the performance of infrared organic photodiodes in the future.

Genomes of leafy and leafless Platanthera orchids illuminate the evolution of mycoheterotrophy.

To improve our understanding of the origin and evolution of mycoheterotrophic plants, we here present the chromosome-scale genome assemblies of two sibling orchid species: partially mycoheterotrophic Platanthera zijinensis and holomycoheterotrophic Platanthera guangdongensis. Comparative analysis shows that mycoheterotrophy is associated with increased substitution rates and gene loss, and the deletion of most photoreceptor genes and auxin transporter genes might be linked to the unique phenotypes of fully mycoheterotrophic orchids. Conversely, trehalase genes that catalyse the conversion of trehalose into glucose have expanded in most sequenced orchids, in line with the fact that the germination of orchid non-endosperm seeds needs carbohydrates from fungi during the protocorm stage. We further show that the mature plant of P. guangdongensis, different from photosynthetic orchids, keeps expressing trehalase genes to hijack trehalose from fungi. Therefore, we propose that mycoheterotrophy in mature orchids is a continuation of the protocorm stage by sustaining the expression of trehalase genes. Our results shed light on the molecular mechanism underlying initial, partial and full mycoheterotrophy.

Development of microsatellite markers for the mycoheterotrophic species Burmannia nepalensis (Miers) Hook.f. based on RAD sequencing.

Mycoheterotrophic plants can derive carbon from fungi rather than from photosynthesis. Habitat destruction and sensitivity to environmental perturbation may result in the loss of biodiversity including genetic variation of mycoheterotrophic plants. Burmannia nepalensis (Miers) Hook.f. (Burmanniaceae) is a mycoheterotrophic plant with a wide distribution across southern China and southern and eastern Asia. As part of our endeavor to reveal population genetic patterns of mycoheterotrophic plants, fifteen microsatellite loci were developed by RAD (restriction site-associated DNA) sequencing in 89 individuals from four populations of B. nepalensis. A total of 49 alleles were amplified. The number of alleles per locus ranged from two to six with an average of 3.3. The observed and expected heterozygosity per population varied from 0.000 to 1.000 and from 0.000 to 0.722, respectively. A transferability test showed that only one to five loci could be cross-amplified successfully in four other congeneric species of Burmannia. These markers can be used to reveal population genetic diversity in B. nepalensis, and will help to elucidate the evolutionary history and to enhance efforts for conservation of mycoheterotrophic plants.

Mitf Involved in Innate Immunity by Activating Tyrosinase-Mediated Melanin Synthesis in Pteria penguin.

The microphthalmia-associated transcription factor (MITF) is an important transcription factor that plays a key role in melanogenesis, cell proliferation, survival and immune defense in vertebrate. However, its function and function mechanism in bivalve are still rarely known. In this research, first, a Mitf gene was characterized from Pteria penguin (P. penguin). The PpMitf contained an open reading frame of 1,350 bp, encoding a peptide of 449 deduced amino acids with a highly conserved basic helix-loop-helix-leucine zipper (bHLH-LZ) domain. The PpMITF shared 55.7% identity with amino acid sequence of Crassostrea gigas (C. gigas). Tissue distribution analysis revealed that PpMitf was highly expressed in mantle and hemocytes, which were important tissues for color formation and innate immunity. Second, the functions of PpMitf in melanin synthesis and innate immunity were identified. The PpMitf silencing significantly decreased the tyrosinase activity and melanin content, indicating PpMitf involved in melanin synthesis of P. penguin. Meanwhile, the PpMitf silencing clearly down-regulated the expression of PpBcl2 (B cell lymphoma/leukemia-2 gene) and antibacterial activity of hemolymph supernatant, indicating that PpMitf involved in innate immunity of P. penguin. Third, the function mechanism of PpMitf in immunity was analyzed. The promoter sequence analysis of tyrosinase (Tyr) revealed two highly conserved E-box elements, which were specifically recognized by HLH-LZ of MITF. The luciferase activities analysis showed that Mitf could activate the E-box in Tyr promoter through highly conserved bHLH-LZ domain, and demonstrated that PpMitf involved in melanin synthesis and innate immunity by regulating tyrosinase expression. Finally, melanin from P. penguin, the final production of Mitf-Tyr-melanin pathway, was confirmed to have direct antibacterial activity. The results collectively demonstrated that PpMitf played a key role in innate immunity through activating tyrosinase-mediated melanin synthesis in P. penguin.

Application of Carbon Nanoparticles in Neck Dissection of Clinically Node-Negative Papillary Thyroid Carcinoma.

PURPOSE: The purpose of this retrospective study was to evaluate the advantages of carbon nanoparticles in neck dissection and to conclude its application in the treatment of clinically node-negative papillary thyroid carcinoma (CN0PTC). METHODS: As a retrospective cohort study, we divided the enrolled patients into two groups, the carbon nanoparticle (CN) group and the control group according to the usage of CN. In the CN group, CN was applied to reveal drainage lymph nodes and the picked LNs were sent for fast frozen testing. If metastasis exits, modified radical lateral lymph node dissection (LLND) was performed. For both groups, prophylactic central lymph node dissection was routinely done. Finally, the demographic information, tumor characteristics, postoperative pathological results, and laboratory data were collected for analysis. RESULTS: A total of 61 CN0PTC were enrolled in this study, 33 in the CN group and 28 in the control group. The black-stained rate for CN was 29/40 (72.5%) with a positive prediction rate of 34.5%. The mainly black-stained region in the lateral neck was level III and possesses the highest lymph node ratio (17.5%). The metastasis that occurred in level VI was 30% and 11.8% in the CN and control groups, respectively (p = 0.058). During the available follow-up, no one showed recurrence. Statistical analysis showed that the CN suspension can significantly reduce the risk of damage to the parathyroid gland (p = 0.001 for hypocalcemia, <0.05; p = 0.047 for hypoparathyroidism, <0.05). CONCLUSION: The lateral neck metastasis in patients with papillary thyroid microcarcinoma in clinical stage cT1aN0 is not rare. CN can help surgeons to distinguish the real person who actually needs LLND. In prophylactic CLND, CN acts as a tracer which makes the parathyroid gland more identifiable and avoids risks of injuries to nerves and glands.

Copper Thiocyanate as an Anode Interfacial Layer for Efficient Near-Infrared Organic Photodetector.

Interfacial modification between the electrode and the overlying organic layer has significant effects on the charge injection and collection and thus the device performance of organic photodetectors. Here, we used copper(I) thiocyanate (CuSCN) as the anode interfacial layer for organic photodetector, which was inserted between the anode and an organic light-sensitive layer. The CuSCN layer processed with ethyl sulfide solution presented similar optical properties to the extensively used anode interlayer of poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS), while the relatively shallow conduction band of CuSCN resulted in a much higher electron-injection barrier from the anode and shunt resistance than those of PEDOT:PSS. Moreover, the CuSCN-based device also exhibited an increased depletion width for the PEDOT:PSS-based device, as indicated by the Mott-Schottky analysis. These features lead to the dramatically reduced dark current density of 2.7 × 10-10 A cm-2 and an impressively high specific detectivity of 4.4 × 1013 cm Hz1/2 W-1 under -0.1 V bias and a working wavelength of 870 nm. These findings demonstrated the great potential of using CuSCN as an anode interfacial layer for developing high-performance near-infrared organic photodetectors.

Linking water environmental factors and the local watershed landscape to the chlorophyll a concentration in reservoir bays.

The frequency of harmful algal blooms caused by eutrophication is increasing globally, posing serious threats to human health and economic development. Reservoir bays, affected by water environment and local watershed landscape, are more prone to eutrophication and algal blooms. The chlorophyll a (Chl a) concentration is an important indicator for the degree of eutrophication and algal bloom. Exploring the complex relationships between water environment and landscape background, and Chl a concentration in the reservoir bays are crucial for ensuring high-quality drinking water from reservoirs. In this study, we monitored Chl a concentrations of 66 bays in Danjiangkou Reservoir and the related water quality parameters (e.g., water temperature, turbidity, nutrients) in waterbodies of these reservoir bays in the storage and discharge periods from 2015 to 2018. Partial least squares-structural equation modeling (PLS-SEM) was used to quantify the relationship between water environmental factors and watershed landscapes, and Chl a concentrations in reservoir bays. The results showed that mean Chl a concentration was higher in storage period than that in discharge period. Two optimal PLS-SEMs explained 66.8% and 53.6% of Chl a concentration variation in the storage and discharge periods, respectively. The net effect of water chemistry on Chl a concentration was more pronounced during the discharge period (total effect = 0.61, 37% of the total effect on Chl a), while the net effect of land-use composition on Chl a concentration was more significant during the storage period (total effect = 0.57, 30% of the total effect on Chl a). The landscape pattern had significant indirect effects on Chl a concentration, especially during the discharge period (indirect effect = -0.31, 19% of the total effect on Chl a). Our results provide valuable information for managers to make rational decisions, thereby contributing to the prevention of eutrophication and algal blooms in reservoir bays.

High-Detectivity Non-Fullerene Organic Photodetectors Enabled by a Cross-Linkable Electron Blocking Layer.

The anode interlayer plays a critical role in the performance of organic photodetectors, which requires sufficient electron-blocking ability to simultaneously attain a high photocurrent and low dark current. Here, we developed two cross-linkable polymers, which can be deposited on the top of the widely used poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) and form a robust layer that can effectively suppress the electron injection from the anode under reverse bias. The optimized device with the resulting cross-linkable XP2 exhibited the lowest dark current density of 5.81 × 10-9 A cm-2 at -0.1 V, which is about 2 orders of magnitude lower than the control devices. A remarkable responsivity of 0.5 A W-1 and a detectivity of >1 × 1013 Jones at a near-infrared wavelength of 800 nm were achieved. Of particular importance is that the resulting device exhibited a linear dynamic range of >135 dB associated with a high working frequency that is shorter than typical commercial digital imagers. The planar heterojunction devices demonstrate that the dark current is closely correlated to the charge generation, which relied on the highest occupied molecular orbital energy levels of the developed cross-linked interlays. The Mott-Schottky analysis revealed that the optimized cross-linked interlayer increased the depletion width of the devices.

Improving the Performance of Blue Polymer Light-Emitting Diodes Using a Hole Injection Layer with a High Work Function and Nanotexture.

For light-emitting polymers with a deep highest occupied molecular orbital energy level used for polymer light-emitting diodes (PLEDs), the hole injection barrier and hole transport of the anode buffer layer are of vital importance for optimizing electroluminescent performance. In this study, high-work-function hole injection layers with nanotextures were achieved by modifying poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) with a perfluorinated ionomer (PFI) and n-butyl alcohol and were used to achieve a single-layer device without a hole transport layer. With such an interlayer, the PLEDs based on PPF-SO25 exhibit remarkable current efficiency over 13.0 cd A-1, which significantly outperform the devices with regular PEDOT:PSS. To our knowledge, this performance is among the best reported for single-layer blue PLEDs. The bias-dependent capacitance curves of these PLEDs suggest a nonuniform surface distribution of PFI. Our findings show that the PFI-modified PEDOT:PSS not only operates as a high-work-function hole injection layer to facilitate hole injection but also as a potential inner scattering medium for light extraction.

Improving the Electroluminescent Performance of Blue Light-Emitting Polymers by Side-Chain Modification.

Blue light-emitting polymers are in urgent demand for new-generation display and solid-state lighting devices fabricated through low-cost wet processing. However, their current performances are far from satisfactory. Here, we developed a series of poly(fluorene-co-dibenzothiophene-S,S-dioxides) (PFSOs) bearing different alkyl chains, alkoxyphenyl chains, or both alkylaryl and alkoxyphenyl side chains. The introduction of alkoxyphenyl groups moderately enhanced the electron-donating ability of the polymers, leading to more balanced charge carrier fluxes. Meanwhile, asymmetric bulky side chains enabled more pronounced variation of molecular conformation while restraining the intermolecular aggregation of polymers, resulting in a lower refractive index, thus facilitating light extraction compared with polymers based on the same two alkyl or alkoxyphenyl side chains. Polymer light-emitting devices based on PFSO-BMD with asymmetric side chains exhibited a maximum luminous efficiency of 8.58 cd A-1, associated with pure blue Commission Internationale de l'Eclairage coordinates of (0.14, 0.14). These findings demonstrated that side-chain modification can be an effective strategy for developing efficient solution-processable blue light-emitting polymers.

Impact of diurnal unsymmetrical warming on soil respiration in an agroecological system of the Lhasa region.

PURPOSE: The impact of diurnal unsymmetrical rise in temperature on soil respiration (Rs) is not fully understood; thus, we explored such a warming influence on Rs in an agroecological system of the Lhasa. MATERIALS AND METHODS: A field warming experiment (C: control; DW: daytime warming; NW: nighttime warming; DW+NW: daytime plus nighttime warming) was carried out in a naked barley ecological system. RESULTS AND DISCUSSION: The DW, NW and DW+NW treatments dramatically increased soil temperature and decreased soil moisture but did not markedly modify Rs. The effects of DW and NW on soil respiration sensitivity (Q10) during the daytime and nighttime were different; they had no effects on daytime Q10 of Rs, but a significant inhibitory effect on nighttime Q10 of Rs. CONCLUSIONS: A diurnal unsymmetrical rise in temperature brought about different results for the Q10 of Rs but did not cause changes in Rs under different experimental treatments in agroecological systems of the Lhasa.

High-Performance All-Polymer Photodetectors via a Thick Photoactive Layer Strategy.

To achieve high detectivity in all-polymer photodetectors (all-PPDs), a thick-film photoactive layer is favored because it can effectively suppress the dark current density. However, if the photoactive layer of the film is too thick, it leads to reduced responsivity owing to increased recombination loss. We developed high-performance all-PPDs by using a narrowband-gap p-type polymer NT40 and an n-type polymer poly{[ N, N'-bis(2-octyldodecyl)-naphthalene-1,4,5,8-bis(dicarboximide)-2,6-diyl]- alt-5,5'-(2,2'bithiophene)} as the photoactive layer. The high charge carrier mobility of both copolymers enabled a photoactive layer thickness of 300 nm, leading to an ultralow dark current density of 4.85 × 10-10 A cm-2, a detectivity of 2.61 × 1013 Jones, a high responsivity of 0.33 A W-1 at 720 nm, and a bias of -0.1 V. The detectivity achieved >1013 Jones in a wide range from 360 to 850 nm, which is among the highest values so far reported for all-PPDs without extra gains. More importantly, the resultant all-PPDs exhibited a high working frequency over 10 kHz associated with a large linear dynamic range. These findings demonstrate great potential for practical applications of the all-PPDs developed in this work.

Dark Current Reduction Strategy via a Layer-By-Layer Solution Process for a High-Performance All-Polymer Photodetector.

The ideal bulk-heterojunction for high-performance organic photodetectors prefers a morphology with a vertically gradient component to suppress the leaking current. Here, we demonstrate an all-polymer photodetector with a segregated bulk-heterojunction active layer. This all-polymer photodetector exhibits a dramatically reduced dark current density because of its built-in charge blocking layer, with a responsivity of 0.25 A W-1 at a wavelength of approximately 600 nm and specific detectivity of 5.68 × 1012 cm Hz1/2 W-1 as calculated from the noise spectra at 1 kHz. To our knowledge, this is among the best performances reported for photodetectors based on both polymeric donor and acceptor in the photoactive layer. These findings present a facile approach to improving the specific detectivity of polymer photodetectors via a layer-by-layer solution process.

Pax3 Gene Regulated Melanin Synthesis by Tyrosinase Pathway in Pteria penguin.

The paired-box 3 (Pax3) is a transcription factor and it plays an important part in melanin synthesis. In this study, a new Pax3 gene was identified from Pteria penguin (Röding, 1798) (P. penguin) by RACE-PCR (rapid-amplification of cDNA ends-polymerase chain reaction) and its effect on melanin synthesis was deliberated by RNA interference (RNAi). The cDNA of PpPax3 was 2250 bp long, containing an open reading fragment of 1365 bp encoding 455 amino acids. Amino acid alignment and phylogenetic tree showed PpPax3 shared the highest (69.2%) identity with Pax3 of Mizuhopecten yessoensis. Tissue expression profile showed that PpPax3 had the highest expression in mantle, a nacre-formation related tissue. The PpPax3 silencing significantly inhibited the expression of PpPax3, PpMitf, PpTyr and PpCdk2, genes involved in Tyr-mediated melanin synthesis, but had no effect on PpCreb2 and an increase effect on PpBcl2. Furthermore, the PpPax3 knockdown obviously decreased the tyrosinase activity, the total content of eumelanin and the proportion of PDCA (pyrrole-2,3-dicarboxylic acid) in eumelanin, consistent with influence of tyrosinase (Tyr) knockdown. These data indicated that PpPax3 played an important regulating role in melanin synthesis by Tyr pathway in P. penguin.

Coffee-Ring-Free Quantum Dot Thin Film Using Inkjet Printing from a Mixed-Solvent System on Modified ZnO Transport Layer for Light-Emitting Devices.

Inkjet printing has been considered an available way to achieve large size full-color RGB quantum dots LED display, and the key point is to obtain printed film with uniform and flat surface profile. In this work, mixed solvent of 20 vol % 1,2-dichlorobenzene (oDCB) with cyclohexylbenzene (CHB) was used to dissolve green quantum dots (QDs) with CdSe@ZnS/ZnS core/shell structure. Then, by inkjet printing, a flat dotlike QDs film without the coffee ring was successfully obtained on polyetherimide (PEI)-modified ZnO layer, and the printed dots array exhibited great stability and repeatability. Here, adding oDCB into CHB solutions was used to reduce surface tension, and employing ZnO nanoparticle layer with PEI-modified was used to increase the surface free energy. As a result, a small contact angle is formed, which leads to the enhancement of evaporation rate, and then the coffee ring effect was suppressed. The printed dots with flat surface profile were eventually realized. Moreover, inverted green QD-LEDs with PEI-modified ZnO film as electron transport layer (ETL) and printed green QDs film as emission layer were successfully fabricated. The QD-LEDs exhibited the maximum luminance of 12 000 cd/m2 and the peak current efficiency of 4.5 cd/A at luminance of 1500 cd/m2.

An Alkane-Soluble Dendrimer as Electron-Transport Layer in Polymer Light-Emitting Diodes.

Polymer light-emitting diodes (PLEDs) have attracted broad interest due to their solution-processable properties. It is well-known that to achieve better performance, organic light-emitting diodes require multilayer device structures. However, it is difficult to realize multilayer device structures by solution processing for PLEDs. Because most semiconducting polymers have similar solubility in common organic solvents, such as toluene, xylene, chloroform, and chlorobenzene, the deposition of multilayers can cause layers to mix together and damage each layer. Herein, a novel semiorthogonal solubility relationship was developed and demonstrated. For the first time, an alkane-soluble dendrimer is utilized as the electron-transport layer (ETL) in PLEDs via a solution-based process. With the dendrimer ETL, the external quantum efficiency increases more than threefold. This improvement in the device performance is attributed to better exciton confinement, improved exciton energy transfer, and better charge carrier balance. The semiorthogonal solubility provided by alkane offers another process dimension in PLEDs. By combining them with water/alcohol-soluble polyelectrolytes, more exquisite multilayer devices can be fabricated to achieve high device performance, and new device structures can be designed and realized.