Clinical study of sleep related rhythmic movement disorder / 中华神经科杂志

Objective To discuss the clinical and electrophysiological characteristics of sleep related rhythmic movement disorder ( SRMD).Methods We studied the clinical and electrophysiological characteristics of 3 patients diagnosed as SRMD in the Electroencephalography Monitoring Center of Xijing Hospital, Xi′an, China.The 3 patients accorded with diagnostic criteria of SRMD that international classification of sleep disorders-3 edition recommended and were followed up for more than 1 year.Results These 3 male patients were ranging from 6 to 27 years old.The onset age of the patient 1 was 13 years,and the others were 1 year old.The patient 2 became symptom-free at the age of 7.The patient 3 relieved at 2-year-old, but recurred at the age of 21. There was no epileptic seizure discharge in video-electroencephalography of the 3 patients, but synchronous electromyography changes during the attack were mistaken for slow wave.Video-polysomnography showed that numbers of awakenings and arousals index were high.Two patients were treated with clonazepam.One had an obvious curative effect, the other had marked efficacy until added trazodone.Conclusions SRMD can occur not only in infants, but also in adolescents and adults.Patients who have the problems of the sleep quality should be treated.Clonazepam can obviously relieve symptoms and improve sleep quality.Patients who do not have a good effect with clonazepam can try to add trazodone.Video-electroencephalography monitoring and interpreting it correctly are important to the diagnosis of paroxysmal disease.

Application of online teaching platform in clinical neurologypractice / 中华医学教育探索杂志

Objective To evaluate the effect of online teaching platform in clinical neurology prac-tice. Methods The participants were medical undergraduate students in the process of clinical practice in Department of Neurology of XiJing hospital, who were divided into the traditional practice teaching group (n=77) and online platform auxiliary practice teaching group (n=90), respectively carry-ing out the corre-sponding rotation practice teaching . Unified objective structured clinical examination was given to two groups of students to implement departmental rotation examination , and investigation was made among teachers and students on the evaluation of the effect of practice. SPSS 19.0 was used to do t test to the two sets of corresponding evaluation data. Results Total score of departmental rotation examination in online platform auxiliary practice teaching group (69.33 ±2.74) was significantly higher than traditional teaching group (67.23±2.50) (P=0.000). Scores of basic theories, skill and operation, clinical case analysis in online platform auxiliary practice teaching group were significantly higher than traditional teaching group (P<0.05). However, medical record writing scores in online platform auxiliary practice teaching group (7.39± 1.09) were significantly lower than the traditional teaching group (8.03±1.03) (P=0.000). Teachers' eval-uation of practice effect was obviously higher in online platform auxiliary practice teaching group (12.33± 0.52) than that in traditional teaching group (10.67 ±1.03) (P=0.005). Students' evaluation of interest in learning, content arrangement, teachers' sense of responsibility, evaluation of teaching ability, practice effect were obviously higher than that of traditional teaching group (P<0.05). Conclusion This study preliminary has achieved good practical effect by using online auxiliary teaching platform, and got high evaluation by both teachers and students. Inter-active communication and sharing of resources is easy in the online plat-form of practice teaching . On-line platform will help students develop with clinical neurology diagnosis thinking.

Detection of the four major human herpesviruses simultaneously in whole blood and cerebrospinal fluid samples by the fluorescence polarization assay.

OBJECTIVES: Herpes simplex virus type 1/2 (HSV-1/-2), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) correlate strongly with infections of the central nervous system. The objective of this study was to develop a method for the simultaneous detection of HSV-1/-2, CMV, and EBV DNA by the fluorescence polarization assay based on asymmetric polymerase chain reaction (PCR) and hybridization. METHODS: DNA of HSV-1/-2, CMV, and EBV was amplified in an asymmetric PCR by a universal primer system. The amplicons were then detected by the fluorescence polarization assay. In this method, the probes for HSV-1/-2, CMV, and EBV hybridized with their respective target amplicons, and the hybridization resulted in an increase in the fluorescence polarization values. Infections of HSV-1/-2, CMV, and EBV were determined by the increased fluorescence polarization values. The DNA extracted from whole blood and cerebrospinal fluid samples was subjected to fluorescence polarization and a previously published multiplex PCR assay in parallel. RESULTS: Compared to the multiplex PCR assay, no significant difference in the numbers of samples positive for the human herpesviruses was identified by the fluorescence polarization assay. CONCLUSIONS: The fluorescence polarization assay presented in this study is a reliable, convenient, and cost-effective diagnostic tool that allows the detection of the four major human herpesviruses.

DISTRIBUTION OF CALCITONIN GENE-RELATED PEPTIDE IMMUNOREACTIVE NERVE FIBERS IN THE SPLEEN OF RAT / 解剖学报

In this study, the distribution of calcitonin gene-related peptide immunoreactive (CGRP-IR) nerve fibers in the spleen of the rat was investigated by using the immunohistochemical ABC method combined with glucose oxidase-DAB-nickel enhancement technique. The results showed that the rat spleen was richly innervated by CGRP-IR fibers. The nerve fibers containing CGRP were mainly distributed in the red pulp, especially surrounding the splenic sinusoid and small blood vessels. They were also observed in the lymphoid tissue of marginal zone, and occasionally in the periarterial lymphatic sheath (PALS). The positive fibers distributed in the spleen, which suggested that the fibers might modulate the development and function of splenic lymphocytes.

DISTRIBUTION OF NEUROPEPTIDE Y IMMUNOREACTIVE FIBERS IN THE SPLEEN OF RAT / 解剖学报

The distribution of neuropeptide Yimmunoreactive (NPY-IR) fibers in the spleen of rat was investigated by using the immunohistochemical ABC method combine with glucose oxidase-DAB-nikel enhancement technique. The results demonstrated that the rat spleen is richly innervated by NPY-IR fibers. The NPY immunoreactive nerve fibers are mainly distributed along the branches of the arteries, especially surrounding the central arterioles. They are also observed in the connective tissue capsule, the lymphoid tissue of the white pulp, red pulp and marginal zone and around the splenic sinusoid. The positive fibers are closely related to the blood vessels and lymphocytes in the spleen, which suggested that these fibers may regulate the development and function of splenic lymphocytes. NPY may have a direct or an indirect effect on regulating blood circulation of the spleen and the lymphocytes.