[Avian diversity and bird strike risk at Fuyang Airport].

From June 2008 to January 2010, a survey of avian communities was conducted in five habitats (grassland, farmland, town, wetland, and woodland) at Fuyang Airport and its surrounding areas, with the diversity indices in different seasons and different habitats analyzed. A total of 122 avian species belonging to 15 orders and 40 families were recorded. At Fuyang Airport, the avian species number was significantly higher in summer and autumn than in winter and spring, the avian density was the highest in autumn, and the Shannon diversity index and Pielou evenness index were the highest in summer. Among the five habitats at the Airport and its surrounding areas, woodland had the greatest avian species number and density, and the woodland, wetland, and farmland had higher Shannon diversity index than grassland and town. The most dangerous avian species to the airplanes at Fuyang Airport were Passer montanus, Pycnonotus sinensis, Hirundo rustica, Columba livia f. domestica, Pica pica, Streptopelia chinensis, and Sturnus cineraceu.

[The different structure forms of HBx protein influence their intracellular distribution in hepatocellular carcinoma HepG2 cells].

AIM: Different eukaryotic expression vectors were selected in this research, and then we respectively cloned and constructed recombinant plasmids contained HBX gene or its different fusion forms with GFP. We are aimed to explore the influence of the HBX protein with different structure on its intracellular localization. METHODS: Flag-HBX gene was amplified from pcDNA3.0-HBX plasmid in our laboratory, cloned into pMD-18T vectors, sequenced.and then subcloned into different vectors. After right sequenced, respective recombinant plasmids of pFlag- HBX-IRES2-EGFP, pEGFP-C3-Flag-HBX and pFlag-HBX-EGFP-N3 were transiently transfected into hepatoma HepG-2 cells. The intra-cellular localizations and distributions of HBX protein were examined by indirect immunofluorescence. RESULTS: Three different Flag-HBX eukaryotic expression vectors were successfully constructed. After transfection of them into HepG2 cells respectively, indirect immunofluorescence demonstrated that HBX protein fused with GFP in different forms show distint intra-cellular distribution characteristics. CONCLUSION: We have provided significant experimental evidences for research of the role of HBX protein in the development of hepatocellular carcinoma, and especially the references for explanation of the outcomes in vitro transfection experiments.