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1.
Genet Mol Res ; 15(3)2016 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-27525930

RESUMO

A total of 48 crossbred Bamei pig carcasses were divided into three groups (A, 60-69 kg; B, 70-79 kg; and C, 80-90 kg) to investigate the influence of carcass weight on meat quality. The intramuscular fat content of the three groups increased from 2.20% (Group A) to 4.14% (Group C). Group B had higher drip loss (6.83%, P < 0.05) than the other two groups. Warner-Bratzler shear force decreased with increasing weight (61.16 > 51.63 > 43.64 N, P < 0.05). No significant differences were observed in meat color, cooking percentage, and water holding capacity among the three groups. The polyunsaturated fatty acids/saturated fatty acids ratio in group B (0.23) was significantly higher than that in the other two groups. In conclusion, our results suggested that a carcass weight of 70-79 kg is suitable for the production of Bamei pigs.


Assuntos
Carne/normas , Adiposidade , Aminoácidos/metabolismo , Animais , Peso Corporal , Qualidade dos Alimentos , Músculo Esquelético/anatomia & histologia , Músculo Esquelético/metabolismo , Sus scrofa
2.
Genet Mol Res ; 15(2)2016 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-27323191

RESUMO

The narrow genetic variation present in common wheat (Triticum aestivum) varieties has greatly restricted the improvement of crop yield in modern breeding systems. Alien addition lines have proven to be an effective means to broaden the genetic diversity of common wheat. Wheat-rye addition lines, which are the direct bridge materials for wheat improvement, have been wildly used to produce new wheat cultivars carrying alien rye germplasm. In this study, we investigated the genetic and epigenetic alterations in two sets of wheat-rye disomic addition lines (1R-7R) and the corresponding triticales. We used expressed sequence tag-simple sequence repeat, amplified fragment length polymorphism, and methylation-sensitive amplification polymorphism analyses to analyze the effects of the introduction of alien chromosomes (either the entire genome or sub-genome) to wheat genetic background. We found obvious and diversiform variations in the genomic primary structure, as well as alterations in the extent and pattern of the genomic DNA methylation of the recipient. Meanwhile, these results also showed that introduction of different rye chromosomes could induce different genetic and epigenetic alterations in its recipient, and the genetic background of the parents is an important factor for genomic and epigenetic variation induced by alien chromosome addition.


Assuntos
Secale/genética , Triticum/genética , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Metilação de DNA , DNA de Plantas , Epigenômica , Etiquetas de Sequências Expressas , Técnicas de Transferência de Genes , Genoma de Planta , Melhoramento Vegetal
3.
Genet Mol Res ; 13(1): 660-9, 2014 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-24615031

RESUMO

In this study, a new disomic addition line, 12-5-2, with 44 chromosomes that was derived from BC3F2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. 12-5-2 was immune to both powdery mildew and stripe rust and has stable fertility. Fluorescence in situ hybridization and C-banding revealed that 12-5-2 was a 1U(b) disomic addition line (ADL1U(b)). The seed storage protein electrophoresis showed that 12-5-2 presented all high molecular weight glutenin subunits (7 + 8 and 2 + 12) of CN19 and 2 new subunits that were designated Ux and Uy. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness of 12-5-2 were significantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1U(b)-specific polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1041, TNAC1071, TNAC1-01, and TNAC1-04, were also obtained. The new ADL1U(b) 12-5-2 could be a valuable source for wheat improvement, especially for wheat end-product quality and resistance to disease.


Assuntos
Cromossomos de Plantas/genética , Glutens/genética , Triticum/genética , Grão Comestível/genética , Glutens/metabolismo , Humanos , Hibridização Genética , Hibridização in Situ Fluorescente , Subunidades Proteicas/genética
4.
Genet Mol Res ; 12(4): 5766-73, 2013 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-24301945

RESUMO

Prolactin (PRL) plays central roles in mammals' reproduction, gland development, milk secretion, and the expression of milk protein genes. In dairy cattle, the PRL gene is a potential quantitative trait locus and genetic marker related to milk performance traits. Here, a total of 586 randomly selected Chinese Holstein cows were genotyped for locus PRL-RsaI. One haplotype block containing eight SNPs was identified in the region from intron 3 to intron 4 of the PRL gene in Chinese Holstein cows. One tag SNP (7545 G → A) was selected to represent the haplotype block defined by the genotypic data. The cows with genotype AA of this tag SNP had a higher milk yield at 305 days (8457 ± 938 kg) than the cows with GA (7537 ± 1278 kg; P < 0.01) or GG (7757 ± 1174 kg; P < 0.05). This suggests that the haplotype block examined in this study contains important markers for milk production traits in Chinese Holstein cows.


Assuntos
Bovinos/genética , Lactação/genética , Prolactina/genética , Locos de Características Quantitativas , Animais , Animais Endogâmicos , Feminino , Polimorfismo de Nucleotídeo Único
5.
Genet Mol Res ; 12(3): 3375-82, 2013 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-24065678

RESUMO

The traits particularly important for milk production include milk yield, protein percentage, fat percentage, and the somatic cell score. Alpha-lactalbumin (α-LA) is an important whey protein of cow milk, and is also present in the milk of many other mammalian species. In this study, we analyzed the genetic polymorphisms of the α-LA gene and their relationship to milk production traits (milk yield, protein percentage, fat percentage, and somatic cell score) in Chinese Holstein cows. The goal of this study was to contribute further molecular genetic information related to dairy cattle, to determine the molecular markers that are most closely linked with milk production traits, and to provide a scientific basis for the improvement of economically relevant traits in cows. Fluorescence-based conformation-sensitive gel electrophoresis, DNA sequencing, and ligation detection reaction techniques were used to analyze genetic variations of the α-LA gene (5'-UTR, exons 1, 2, 3, 4, and 3'-UTR) in 923 Chinese Holstein cows. One novel single nucleotide polymorphism (SNP), α-LA2516, was identified in exon 4 of the α-LA gene. Allele frequencies were as follows: T 0.674, C 0.326. Association analysis revealed that α-LA2516 was not associated with milk yield, protein percentage, fat percentage, or somatic cell score (P > 0.05). These findings suggest that the SNP α-LA2516 in the α-LA gene likely does not have potential as a molecular marker for milk production traits in Chinese Holstein cows.


Assuntos
Estudos de Associação Genética , Lactalbumina/genética , Proteínas do Leite/genética , Leite , Animais , Bovinos , China , Polimorfismo de Nucleotídeo Único
6.
Genet Mol Res ; 10(4): 2511-6, 2011 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-22009863

RESUMO

The most important traits of Chinese Liaoning cashmere goat fiber are fiber diameter, weight, and length. We looked for polymorphisms and their possible association with cashmere fiber traits in the 5' upstream region (5' UTR) of the prolactin receptor gene (PRLR), which encodes an anterior pituitary peptide hormone involved in different physiological activities; it is the principal endocrine regulator in pelage replacement in mammals. A novel single-nucleotide polymorphism (SNP) was found in the 5' UTR of PRLR by PCR-RFLP in an analysis of 590 goats. Two genotypes (CC and CT) were observed. The frequencies of allele C and T were 0.93 and 0.07, respectively. Association analysis revealed that the PRLR 5' UTR polymorphism (SNP5) was significantly associated with cashmere fiber weight and diameter. This novel SNP in hircine PRLR has potential as a molecular marker for cashmere fiber weight and diameter in Liaoning cashmere goats.


Assuntos
Regiões 5' não Traduzidas , Cabras/genética , Polimorfismo de Nucleotídeo Único , Característica Quantitativa Herdável , Receptores da Prolactina/genética , , Alelos , Animais , Feminino , Frequência do Gene/genética , Masculino
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