RESUMO
OBJECTIVE: The purpose of study was to discover the phylogenetic relations and plant barcoding of 17 plants from Huperziaceae. METHODS: Phylogenetic tree of chloroplast trnH-psbA gene of 17 plants from Huperziaceae was constructed by software. RESULTS: It showed that Huperziaceae could be divided into two genera Huperzia and Phlegmariurus and bootstrap value reached 91%. CONCLUSIONS: Holub and Qing' taxonomy was supported and 17 species in Huperziaceae were monophyletic groups and it suggested that trnH-psbA could be used as a DNA barcode to identify plants.
Assuntos
Cloroplastos/genética , Código de Barras de DNA Taxonômico , Genes de Plantas , Huperzia/genética , Primers do DNA , DNA de Plantas/genética , Processamento Eletrônico de Dados/métodos , Huperzia/classificação , Dados de Sequência Molecular , Filogenia , Plantas Medicinais/classificação , Plantas Medicinais/genética , Especificidade da EspécieRESUMO
OBJECTIVE: To research the effect of glaucocalyxin A (GLA) on the level of Th1/Th2 type cytokines in mice. METHOD: By using flow cytometer with CBA software to detect Th1/Th2 type cytokines. RESULT: GLA had insighificant inhibitory effects on Th1 and Th2 cytokines (IL-2, IFN-gamma,TNF-alpha, IL-4 and IL-5) induced by ConA, in which more potential on cytokines from Th1 than those of from Th2 were displayed. However, GLA could produce inhibition on IL-2, IFN-gamma and TNF-alpha and acceleration on IL-4 and IL-5. CONCLUSION: Immunosuppressive effect of GLA is related to the influence the level of Th1/Th2 type cytokines.
Assuntos
Citocinas/metabolismo , Medicamentos de Ervas Chinesas/farmacologia , Isodon , Células Th1/metabolismo , Células Th2/metabolismo , Animais , Diterpenos do Tipo Caurano , Medicamentos de Ervas Chinesas/isolamento & purificação , Feminino , Interferon gama/metabolismo , Interleucina-2/metabolismo , Interleucina-4/metabolismo , Interleucina-5/metabolismo , Isodon/química , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Plantas Medicinais/química , Fator de Necrose Tumoral alfa/metabolismoRESUMO
The purpose of the present study was to examine the effects of calycosin, an isoflavonoid isolated from Astragali Radix, on the impairment of barrier function induced by hypoxia in cultured human umbilical vein endothelial cells. Hypoxia induced an increase in endothelial cell monolayer permeability, indicating endothelial cell barrier impairment. Endothelial barrier dysfunction induced by hypoxia was accompanied by decreases in cytosolic ATP concentration and cAMP level, the development of actin stress fibers and intercellular gap formation, suggesting that the decreases in cytosolic ATP and cAMP levels and rearrangements of F-actin could be associated with an increase in permeability of endothelial monolayers. Application of calycosin inhibited the hypoxia-induced increase in endothelial permeability in a dose-dependent fashion, which is compatible with inhibition of lactate dehydrogenase release, decrease of the fall in ATP and cAMP contents, and improvement of F-actin rearrangements. These findings indicate that calycosin protected endothelial cells from hypoxia-induced barrier impairment by increasing intracellular energetic sources and promoting regeneration of the cAMP level, as well as improving cytoskeleton remodeling.
