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1.
Sci Total Environ ; 939: 173606, 2024 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-38823704

RESUMO

Organic soil amendments have been widely adopted to enhance soil organic carbon (SOC) stocks in agroforestry ecosystems. However, the contrasting impacts of pyrogenic and fresh organic matter on native SOC mineralization and the underlying mechanisms mediating those processes remain poorly understood. Here, an 80-day experiment was conducted to compare the effects of maize straw and its derived biochar on native SOC mineralization within a Moso bamboo (Phyllostachys edulis) forest soil. The quantity and quality of SOC, the expression of microbial functional genes concerning soil C cycling, and the activity of associated enzymes were determined. Maize straw enhanced while its biochar decreased the emissions of native SOC-derived CO2. The addition of maize straw (cf. control) enhanced the O-alkyl C proportion, activities of ß-glucosidase (BG), cellobiohydrolase (CBH) and dehydrogenase (DH), and abundances of GH48 and cbhI genes, while lowered aromatic C proportion, RubisCO enzyme activity, and cbbL abundance; the application of biochar induced the opposite effects. In all treatments, the cumulative native SOC-derived CO2 efflux increased with enhanced O-alkyl C proportion, activities of BG, CBH, and DH, and abundances of GH48 and cbhI genes, and with decreases in aromatic C, RubisCO enzyme activity and cbbL gene abundance. The enhanced emissions of native SOC-derived CO2 by the maize straw were associated with a higher O-alkyl C proportion, activities of BG and CBH, and abundance of GH48 and cbhI genes, as well as a lower aromatic C proportion and cbbL gene abundance, while biochar induced the opposite effects. We concluded that maize straw induced positive priming, while its biochar induced negative priming within a subtropical forest soil, due to the contrasting microbial responses resulted from changes in SOC speciation and compositions. Our findings highlight that biochar application is an effective approach for enhancing soil C stocks in subtropical forests.


Assuntos
Carbono , Carvão Vegetal , Florestas , Solo , Zea mays , Carvão Vegetal/química , Solo/química , Microbiologia do Solo
2.
Ying Yong Sheng Tai Xue Bao ; 34(9): 2383-2390, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37899103

RESUMO

We investigated the effects of maize straw and its biochar application on soil organic carbon chemical composition, the abundance of carbon degradation genes (cbhI) and the composition of cbhI gene community in a Moso bamboo forest, to provide the theoretical and scientific basis for enhancing carbon sequestration. We conducted a one-year field experiment in a subtropical Moso bamboo forest with three treatments: control (0 t C·hm-2), maize straw (5 t C·hm-2), and maize straw biochar (5 t C·hm-2). Soil samples were collected at the 3rd and 12th months after the treatment. Soil organic carbon chemical composition, the abundance and community composition of cbhI gene were determined by solid-state 13C NMR, real-time fluorescence quantitative PCR, and high-throughput sequencing, respectively. The results showed that compared with the control, maize straw treatment significantly increased the content of O-alkyl C and decreased aromatic C content, while maize straw biochar treatment showed an opposite effect. Maize straw treatment significantly increased the abundance of cbhI gene and the relative abundance of Penicillium, Gaeumannomyces and Marasmius. However, maize straw biochar treatment reduced the abundance of this gene. The relative abundance of dominant cbhI in soils was positively correlated with the content of O-alkyl C and negatively correlated with the content of aromatic C. Results of redundancy analysis showed that maize straw treatment had a significant effect on the microbial community composition of cbhI gene by changing soil O-alkyl C content, while maize straw biochar affected the microbial community composition of cbhI gene by changing soil pH, organic carbon, and aromatic C content. Maize straw biochar treatment was more effective in increasing soil organic carbon stability and reducing microbial activity associated with carbon degradation in the subtropical Moso bamboo forest ecosystem compared with maize straw treatment. Therefore, the application of biochar has positive significance for maintaining soil carbon storage in subtropical forest ecosystems.


Assuntos
Carbono , Microbiota , Carbono/análise , Zea mays , Solo/química , Carvão Vegetal/química , Poaceae , Florestas , Compostos Orgânicos
3.
Sci Total Environ ; 801: 149717, 2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34425443

RESUMO

Despite fresh and pyrogenic organic matter have been widely used as amendments to improve soil organic carbon (SOC) storage, mineralization that links to C quality and soil temperature, microbial community composition and enzyme activity remain poorly understood. This study aims to explore the effects of amendments (bamboo leaves and its biochar) and incubation temperature on mineralization, and disentangle the relationships of SOC mineralization with chemical composition of SOC, labile organic C, microbial community composition, and activities of enzymes in a subtropical bamboo forest soil. Results showed that cumulative soil CO2 emissions ranked as bamboo leaf (Leaf) > bamboo leaf biochar (Biochar) > Control, regardless of the incubation temperature. Compared to the control, the Leaf treatment markedly increased, whereas the Biochar treatment decreased, the temperature sensitivity of SOC mineralization (P < 0.05). The cumulative soil CO2 emission was positively correlated (P < 0.05) with water-soluble organic C (WSOC), microbial biomass C (MBC), O-alkyl C and alkyl C contents, and activities of ß-glucosidase and dehydrogenase, but negatively correlated (P < 0.01) with aromatic C content, regardless of the incubation temperature. This indicated that the lower SOC mineralization rate and lower temperature sensitivity in the Biochar (cf. Leaf) treatment were intimately associated with the lower WSOC, MBC, O-alkyl C content, and ß-glucosidase and dehydrogenase activities, and higher aromatic C content in the Biochar. The high relative abundance of bacteria relating SOC mineralization included Rhizobiales, Sphingobacteriales and JG30-KF-AS9, whereas that of fungi included Eurotiales, Sordariales, Agaricales and Helotiales. Our results revealed that the application of pyrogenic organic matter, as compared to the application of fresh organic matter, can reduce SOC mineralization and its temperature sensitivity in a subtropical forest soil by limiting the availability of C and microbial activity, and thus has a great potential for maintaining soil carbon stock in subtropical forest ecosystems.


Assuntos
Microbiota , Solo , Carbono , Carvão Vegetal , Florestas , Microbiologia do Solo
4.
Acta Crystallogr D Biol Crystallogr ; 61(Pt 4): 416-22, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15805596

RESUMO

A genetically engineered humanized C(H)2-domain-deleted monoclonal antibody lacking any interchain-hinge disulfide bonds has been crystallized in the presence of detergent in a form suitable for X-ray diffraction analysis. The crystals were grown from 4 M formate along with Triton X-100 and had P2(1)2(1)2 space-group symmetry, with unit-cell parameters a = 83, b = 224, c = 167 A. The crystals diffract to beyond 2.8 A resolution. A disordered crystal form of larger size and more attractive habit was also grown from 4 M formate, but in the presence of the Anapoe series of detergents. Preliminary X-ray data, in conjunction with atomic force microscopy images, are consistent with asymmetric units consisting of two intact antibodies forming a circular dimeric ring. The crystallizing unit, which must contain a twofold axis, is a toroidal assembly of four antibodies (two dimeric rings). Competition between dimers and tetramers to enter the lattice, along with a unique kind of planar defect of packing, may be responsible for the unusually high defect density and the disorder of the X-ray diffraction pattern exhibited by the second crystal form. An approach to crystallizing proteins showing phase separation, particularly intact antibodies, that uses a preliminary detergent test set is described.


Assuntos
Anticorpos Antineoplásicos/química , Fragmentos de Imunoglobulinas/química , Microscopia de Força Atômica , Antígenos de Neoplasias/imunologia , Cristalização , Cristalografia por Raios X , Detergentes , Glicoproteínas/imunologia , Octoxinol , Engenharia de Proteínas , Estrutura Terciária de Proteína/genética
5.
J Mol Biol ; 347(1): 41-52, 2005 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-15733916

RESUMO

Single-stranded genomic RNAs from four icosahedral viruses (poliovirus, turnip yellow mosaic virus (TYMV), brome mosaic virus (BMV), and satellite tobacco mosaic virus (STMV)) along with the RNA from the helical tobacco mosaic virus (TMV) were extracted using phenol/chloroform. The RNAs were imaged using atomic force microscopy (AFM) under dynamic conditions in which the RNA was observed to unfold. RNAs from the four icosahedral viruses initially exhibited highly condensed, uniform spherical shapes with diameters consistent with those expected from the interiors of their respective capsids. Upon incubation at 26 degrees C, poliovirus RNA gradually transformed into chains of globular domains having the appearance of thick, irregularly segmented fibers. These ultimately unwound further to reveal segmented portions of the fibers connected by single strands of RNA of 0.5-1 nm thickness. Virtually the same transformations were shown by TYMV and BMV RNA, and with heating, the RNA from STMV. Upon cooling, the chains of domains of poliovirus RNA and STMV RNA condensed and re-formed their original spherical shapes. TMV RNAs initially appeared as single-stranded threads of 0.5-1.0 nm diameter but took on the structure of the multidomain chains upon further incubation at room temperature. These ultimately condensed into short, thick chains of larger domains. Our observations suggest that classical extraction of RNA from icosahedral virions produces little effect on overall conformation. As tertiary structure is lost however, it is evident that secondary structural elements are arranged in a sequential, linear fashion along the polynucleotide chain. At least in the case of poliovirus and STMV, the process of tertiary structure re-formation from the linear chain of secondary structural domains proceeds in the absence of protein. RNA base sequence, therefore, may be sufficient to encode the conformation of the encapsidated RNA even in the absence of coat proteins.


Assuntos
Conformação de Ácido Nucleico , RNA Viral/ultraestrutura , Bromovirus/genética , Bromovirus/ultraestrutura , Microscopia de Força Atômica , Poliovirus/genética , Poliovirus/ultraestrutura , RNA Viral/química , Vírus do Mosaico do Tabaco/genética , Vírus do Mosaico do Tabaco/ultraestrutura , Vírus Satélite do Mosaico do Tabaco/genética , Vírus Satélite do Mosaico do Tabaco/ultraestrutura , Tymovirus/genética , Tymovirus/ultraestrutura
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