RESUMO
OBJECTIVE: To investigate the effect of Astragalus mongholicus injection on proliferation and apoptosis in hormone sensitive breast cancer cell lines (MCF-7) with physiological dose E2. METHODS: Cell control group, TAM control group and five different dose Astragalus mongholicus injection groups with physiological dose E2 were set in the study. Proliferation of MCF-7 cells were evaluated with MTT assay, cell apoptotic rate were measured with flow cytometry, DNA ladder and cell cycle were observed. RESULTS: In physiological dose E2, Astragalus mongholicus injection inhibited MCF-7 cells proliferation at all concentration groups. As time lasting, Astragalus mongholicus injection showed better inhibitory effect than TAM (P<0.05). Among 2 x 10(-1) g/mL-2 x 10(-4) g/mL concentration, Astragalus mongholicus injection significantly increased the proliferative percent of G0/G1 and S-phase cell, decreased percent of G2-M phase cell (P<0.05) at 24 hours. After cocultured 72 hours, Astragalus mongholicus injection increased the rate of apoptosis to 16.7% at 2 x 10(-1) g/mL concentration. CONCLUSION: In some dose limit, Astragalus mongholicus injection may inhibit proliferation, induce apoptosis and interrupt caryocinesia at G0-G1 phase or S phase in hormone sensitive (MCF-7) breast cancer cell lines with physiological dose E2.
Assuntos
Antineoplásicos/farmacologia , Astragalus propinquus , Neoplasias da Mama/patologia , Proliferação de Células/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Estradiol/farmacologia , Antineoplásicos/uso terapêutico , Apoptose/efeitos dos fármacos , Neoplasias da Mama/tratamento farmacológico , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Relação Dose-Resposta a Droga , Medicamentos de Ervas Chinesas/uso terapêutico , Feminino , Citometria de Fluxo , Humanos , Fitoterapia , Receptores de Estrogênio , Tamoxifeno/farmacologia , Tamoxifeno/uso terapêuticoRESUMO
Multidrug resistance of breast cancer is one of the most causes of failure in clinical chemotherapy. It is important to find out some safe and effective drugs to reserve multidrug resistance to breast cancer. The effect of some herbs had been identified in vitro. This article mainly reviewed the research progress in reversing multidrug resistance to breast cancer with Chinese herb. If the herb's effects and safety can be testified in vivo by further research, it will be effectively applied in clinic.
