Variation in Pheidole nodus (Hymenoptera: Formicidae) functional morphology across urban parks.

Background: Habitat fragmentation and consequent population isolation in urban areas can impose significant selection pressures on individuals and species confined to urban islands, such as parks. Despite many comparative studies on the diversity and structure of ant community living in urban areas, studies on ants' responses to these highly variable ecosystems are often based on assemblage composition and interspecific mean trait values, which ignore the potential for high intraspecific functional trait variation among individuals. Methods: Here, we examined differences in functional traits among populations of the generalist ant Pheidole nodus fragmented between urban parks. We used pitfall trapping, which is more random and objective than sampling colonies directly, despite a trade-off against sample size. We then tested whether trait-filtering could explain phenotypic differences among urban park ant populations, and whether ant populations in different parks exhibited different phenotypic optima, leading to positional shifts in anatomical morphospace through the regional ant meta-population. Results: Intraspecific morphological differentiation was evident across this urban region. Populations had different convex hull volumes, positioned differently over the morphospace. Conclusions: Fragmentation and habitat degradation reduced phenotypic diversity and, ultimately, changed the morphological optima of populations in this urban landscape. Considering ants' broad taxonomic and functional diversity and their important role in ecosystems, further work over a variety of ant taxa is necessary to ascertain those varied morphological response pathways operating in response to population segregation in urban environments.

The complete mitochondrial genome of Aphaenogaster japonica (Forel, 1911) (Hymenoptera: Formicidae).

Aphaenogaster japonica (Forel, 1911) is an omnivorous ant that is widely distributed in eastern Asia. The mitochondrial genome of A. japonica reported here was 18,607 bp in length, consisting of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a control region. The base composition was AT biased (the GC ratio is 18.9%). With A. japonica added, we obtain weak evidence that the sister group of the Stenammini group, including Aphaenogaster, is the Myrmicini group. Therefore, the Stenammini and Myrmicini groups may be not a robust monophyletic group, unlike the previous results based on the complete mitochondrial genome.

The complete mitochondrial genome of Pheidole nodus (Smith, 1874) (Hymenoptera: Formicidae).

Pheidole nodus (Smith, 1874) belongs to a famously hyperdiverse and ecologically dominant ant genus. The mitochondrial genome of P. nodus is 15,579 bp in length, and the overall base composition is 78.6% AT. It includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNAs, and a control region. Phylogenetic trees show that P. nodus is more closely related to Wasmannia than to Atta. These sequence data will play an important role in the investigation of the phylogenetic relationships and taxonomy of the group Attini.

The complete mitochondrial genome of Tetramorium tsushimae (Emery, 1925) (Hymenoptera: Formicidae).

Tetramorium tsushimae (Emery, 1925) is an omnivorous ant species native to East Asia and has been introduced to North America. The mitochondrial genome of T. tsushimae is 19,207 bp in length with an A + T content of 81.3% and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNAs, and a control region. This sequence data would play an important role in the investigation of mitochondrial evolution of the subfamily Myrmicinae.

Comparing Ant Assemblages and Functional Groups across Urban Habitats and Seasons in an East Asia Monsoon Climate Area.

China's East Asia monsoon zone is undergoing rapid land-use conversion and urbanization. Safeguarding remaining biodiversity requires reducing, mitigating, and/or eliminating the negative impacts of human-induced landscape modification. In this study, we sampled ground-dwelling ants at 40 plots over 12 continuous months in a suburban area in southwestern China to examine whether and how vegetation composition and habitat fragmentation affected species richness and assemblage composition for the general ant community and, specifically, for principal functional groups (including Opportunists and Generalized Myrmicinae). Warmer seasons were associated with a higher capture rate for all functional groups. Patterns of ant species richness among Opportunists were more sensitive to vegetation and fragmentation than for Generalized Myrmicinae, and these effects generally varied with season. Patterns of ant assemblage composition for Opportunists were exclusively sensitive to vegetation, whereas Generalized Myrmicinae were sensitive to both vegetation and fragmentation with variation among seasons. Overall, our findings highlight the important role of seasonality, vegetation composition, and habitat fragmentation in mediating the impacts of human-induced landscape modification on urbanized ant communities, which make an essential functional contribution to biodiversity in the East Asia monsoon zone.

Prosecution records reveal pangolin trading networks in China, 2014-2019.

In a precautionary response to the current coronavirus (COVID-19) pandemic, China's Ministries permanently banned eating and trading in terrestrial wild (non-livestock) animals on 24 February 2020, and extensively updated the list of Fauna under Special State Protection (LFSSP) in 2020 and 2021, in which pangolins (Manidae spp.) were upgraded to the highest protection level. Examining 509 pangolin prosecution records from China Judgements online prior to these changes (01/01/14-31/12/19), we identified that Guangdong, Guangxi and Yunnan Provinces were hotspots for trade in whole pangolins and their scales. Interrupting trade in these three principal southern provinces would substantially fragment the pangolin trade network and reduce supply of imports from other south-east Asian countries. In the context of the revised legislation and strategies intended to prevent wildlife trade, we conclude that targeting interventions at key trade nodes could significantly reduce illegal trade in pangolins, and that this approach could also be effective with other taxa.

Animal sales from Wuhan wet markets immediately prior to the COVID-19 pandemic.

Here we document 47,381 individuals from 38 species, including 31 protected species sold between May 2017 and November 2019 in Wuhan's markets. We note that no pangolins (or bats) were traded, supporting reformed opinion that pangolins were not likely the spillover host at the source of the current coronavirus (COVID-19) pandemic. While we caution against the misattribution of COVID-19's origins, the wild animals on sale in Wuhan suffered poor welfare and hygiene conditions and we detail a range of other zoonotic infections they can potentially vector. Nevertheless, in a precautionary response to COVID-19, China's Ministries temporarily banned all wildlife trade on 26th Jan 2020 until the COVID-19 pandemic concludes, and permanently banned eating and trading terrestrial wild (non-livestock) animals for food on 24th Feb 2020. These interventions, intended to protect human health, redress previous trading and enforcement inconsistencies, and will have collateral benefits for global biodiversity conservation and animal welfare.

Understanding wildlife crime in China: Socio-demographic profiling and motivation of offenders.

Wildlife crime presents a growing threat to the integrity of ecological communities. While campaigns have raised consumer awareness, little is known about the socio-demographic profile of wildlife offenders, or how to intervene. Using data from China Judgements Online (2014-2018), we documented 4,735 cases, involving 7,244 offenders who smuggled, hunted, transported, sold and/or purchased protected species in contravention of China's Criminal Law. Offenders were predominantly men (93.0% of 7,143 offenders), aged 30-44 (43.9% of 4,699), agricultural workers (48.4% of 3,960), with less schooling (78.6% of 4,699 < senior secondary school). Socio-economic profiles related to crime seriousness, the type of illegal activity, motivation and taxon involved. These generalizations reveal scope to tailor specific intervention and mitigation approaches to offender profiles, through public information campaigns, proactive incentives opposed by punitive disincentives, and provision of alternative incomes.

China's online parrot trade: Generation length and body mass determine sales volume via price.

The wildlife trade threatens global biodiversity and animal welfare, where parrots are among the taxa most frequently traded, supplying exotic pets and captive breeders worldwide. Using phylogenetic path analysis, we examine how biological factors interact with price to influence online protected parrot trade volumes in China, using transactions recorded for 46 species (n = 5862 individuals). Trade was greatest in smaller, faster breeding species that commanded a lower price. This price effect followed the economic law of demand, with Relatively Inelastic Demand (-0.758), outweighing indicators of 'quality' such as body coloration, and conservation status. We identify two areas of concern: those larger, slower-breeding, rarer species, even though sold at lower numbers, may be at conservation risk if harvested from the wild. In contrast, the sheer numbers (over 90% of the individuals were under median generation length, body mass and/or price) and ready availability of smaller and more common species comprises a substantial overall animal welfare issue, given that the capture, importation, or captive breeding of many parrot species in China is illegal and thus unregulated. Our investigation highlights the importance of properly understanding the internal relations among drivers of wildlife trade to inform appropriate management.

First record of Leptospira and Blastocystis infections in captive flying squirrels (Trogopterus xanthipes) from Enshi County, China.

In traditional Chinese medicine, the feces of flying squirrels have long been used to promote blood circulation and relieve bodily stasis. However, the excrement of flying squirrels may harbor zoonotic agents that could be hazardous to public health. To understand the occurrence of bacterial and parasitic infections in this species, we investigated selected zoonotic pathogens including Leptospira and Blastocystis in the urine and feces of flying squirrels in China. Urine and fecal samples from flying squirrels were collected from a family-owned flying squirrel farm located in Enshi County, Hubei Province in China. Leptospira and Blastocystis DNA was extracted from the urine and feces of flying squirrels, and used as targets for PCR amplification, using different specific primers. PCR amplification and DNA sequencing showed that 4.4% (3/69) of flying squirrels were positive for Leptospira, while 30.4% (21/69) of the animals were positive for Blastocystis. Notably, 1.4% (1/69) of flying squirrels were found to be co-infected with Leptospira and Blastocystis. Sequence analyses allowed for the detection of 3 Blastocystis subtypes (ST1, ST3 and ST13), and mixed infections of Blastocystis subtype 1 and subtype 3 were found in 4.4% (3/69) of flying squirrels. Phylogenetic analysis of the 16S ribosomal RNA gene (rrs2), the flagellin B gene (flaB), and outer membrane lipoprotein lipL32 gene (LipL32) sequences indicated that the Leptospira species detected in the study was L. interrogans. We concluded that flying squirrels from central China were infected with Leptospira and Blastocystis, suggesting that these animals can be a source of infection for their owners, and using fresh excrement from this animal as traditional medicine could be risky to human health. To the best of our knowledge, this is the first report of Leptospira and Blastocystis infection in flying squirrels from Enshi County, China. Our findings provide new data on the epidemiology of these pathogens in this region.

Fertilizer application in rural cropland drives cadmium enrichment in bats dwelling in an urban area.

The transfer of pollutants from chemical fertilizers through food webs within cropland is well documented; however, its impacts on the wild animals that forage on croplands but roost in other locations remain poorly understood. The potential for this cross-ecosystem 'spillover' of pollutants is greatest for bats, some of which exploit urban settlements as roosting niches but must travel long distances to reach croplands as foraging niches. Here, we used hairs from a colony of insectivorous bats, Chinese Noctule (Nyctalus plancyi), from an urban area in Southwest China to assess whether exposure to heavy metals/metalloids by the bats varied from 1975 to 2016. Historical changes occurred in hair cadmium (Cd) concentrations in adult females, which was exclusively explained by the regional fertilizer application intensity (FAI), even considering the potential impacts of Cd emissions in urban areas, as indicated by camphor trees (Cinnamomum camphora) near the bats' roosting niche, and the potential impacts of Cd in industrial wastewater, as documented in authorized databases. Therefore, the data from this bat colony, as urban dwellers, indicates Cd accumulation and cross-ecosystem transfer from rural croplands to an urban area.

[Allometry of scales in Chinese pangolins (Manis pentadactyla) and Malayan pangolins (Manis javanica) and application in judicial expertise].

Pangolins are unique mammals in that they possess scales that serve a protective biological function. As an important raw material of traditional medicine, illegal trades of these scales are frequent and difficult to investigate or prosecute. We used allometric models of dry weight of scales to compare 35 Chinese pangolins (Manis pentadactyla) and 119 Malayan pangolins (Manis javanica). Our results showed that the dry weight of scales increases significantly faster with the length of head and body in Malayan pangolins (P=0.005), while dry weight of scales is positive (slope=3.725) in Malayan pangolins but isometric (slope=3.105) in Chinese pangolins. The differences in morphology between these species may reflect an evolutionary adaptation to different environments; Malayan pangolins in tropical regions appear to suffer from greater predation pressure than Chinese pangolins in temperate regions. We advise the conversion standards between dry weight of scales and number of individuals as 573.47 g in Chinese pangolins and 360.51 g in Malayan pangolins respectively, and when two are mixed together, average above two parameters of the median at 466.99 g. We propose these measurements be used as judicial evidences in forensic identification of related cases.