Human Umbilical Cord Mesenchymal Stem Cells-Derived Extracellular Vesicles for Rat Jawbone Regeneration in Periapical Periodontitis.

Extracellular vesicles derived from mesenchymal stem cells (MSCs-EVs) have great potential for bone remodeling and anti-inflammatory therapy. For the repair and reconstruction of inflammatory jawbone defects caused by periapical periodontitis, bone meal filling after debridement is commonly used in the clinic. However, this treatment has disadvantages such as large individual differences and the need for surgical operation. Therefore, it is of great significance to search for other bioactive substances that can promote jawbone regeneration in periapical periodontitis. Herein, it is found that CT results showed that local injection of human umbilical cord mesenchymal stem cells-derived extracellular vesicles (HUC-MSCs-EVs) and bone meal filling into the alveolar bone defect area could promote bone tissue regeneration using a rat model of a jawbone defect in periapical periodontitis. Histologically, the new periodontal tissue in the bone defect area was thicker, and the number of blood vessels was higher by local injection of HUC-MSCs-EVs, and fewer inflammatory cells and osteoclasts were formed compared to bone meal filling. In vitro, HUC-MSCs-EVs can be internalized by rat bone marrow mesenchymal stem cells (BMSCs), enhancing the ability for proliferation and migration of BMSCs. Additionally, 20 µg/mL HUC-MSCs-EVs can facilitate the expression of osteogenic genes and proteins including runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteopontin (OPN). In summary, in vivo and in vitro experiments showed that HUC-MSCs-EVs can promote bone regeneration in periapical periodontitis, and the effect of tissue regeneration is better than that of traditional bone meal treatment. Therefore, local injection of HUC-MSCs-EVs may be an effective method to promote jawbone regeneration in periapical periodontitis.

Designing Inorganic-Organic Dual-Acid Deep Eutectic Solvents for Synergistically Enhanced Extractive and Oxidative Desulfurization.

Acidic deep eutectic solvents (DESs) have been considered desirable extractants and catalysts for desulfurization. However, their hydrogen bond donors (HBDs) are usually sole organic acids, which are not conducive to efficient green catalysis. Herein, a novel inorganic-organic dual-acid DES (DADES) was reported for efficient extractive and oxidative desulfurization. Benefiting from the physical interaction among the three components in a DADES, a transparent homogeneous liquid can be obtained even though inorganic acid (boric acid, BA) and organic acid (acetic acid, AA) can be immiscible. Furthermore, the dual-acid HBD can increase the acidity of the DADES and reduce its viscosity, accelerating its mass transfer efficiency and enhancing its catalytic activity. With 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) as the hydrogen bond acceptor, [Bmim]Cl/BA/0.3AA effectively activated hydrogen peroxide and achieved sulfur removal of 96.6% at 40 °C. Furthermore, the universality of the synergistic effect in various DADESs was confirmed by the modulation of the types of organic acids. This study not only motivates the construction of more intriguing novel DESs based on the DADES concept but also highlights their potential in clean fuel production.