RESUMO
A simple restricted-access media (RAM) HPLC method for simultaneous determination of the lactone and carboxylate forms of 10-hydroxycamptothecin (HCPT) in human serum was established. Using a RAM Hisep analytical column, serum samples were directly injected into the HPLC system. The eluted peaks of two forms of HCPT were monitored with a fluorescence detector. The separation was completed in 17 min. The linear range was 20-1000 ng/ml, intra-day and inter-day variations being less than 5%. The kinetic equation was introduced according to the analytical results. The equation shows that the course of the HCPT lactone form converting to carboxylate form in human serum at 4 degrees C is a first-order kinetic course. The concentration of each form at the moment of sampling was calculated by extrapolation.
Assuntos
Antineoplásicos/sangue , Camptotecina/análogos & derivados , Camptotecina/sangue , Ácidos Carboxílicos/química , Cromatografia Líquida de Alta Pressão/métodos , Lactonas/química , Antineoplásicos/química , Calibragem , Camptotecina/química , Humanos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Espectrometria de FluorescênciaRESUMO
AIM: To compare restricted-access media high performance liquid chromatographic (RAM-HPLC) method with fluorescence polarization immunoassay (FPIA) for analysis of carbamazepine (CBZ) in human blood. METHODS: An RAM-HPLC method was established for the determination of CBZ in plasma. RESULTS: The two methods do not need sample clean-up prior to analysis and they have almost 100 % recovery and good reproducibility. There is a good correlation between the CBZ concentration in venous plasma samples determined by FPIA and that in both venous and fingertip plasma samples obtained by RAM-HPLC, the correlation coefficients being 0.989 and 0.995, respectively. It is shown by t-test that the data sets of venous and fingertip plasma samples given by RAM-HPLC are consistent with each other but significantly different from the results obtained by FPIA. CONCLUSION: Both direct injection RAM-HPLC and FPIA may be applied in determining CBZ in therapeutic drug monitoring (TDM). FPIA is well-suited to the routine TDM. RAM-HPLC is more useful in TDM related research and especial cases.
