Phylogeography and Genetic Structure of the Bush Cricket Decma fissa (Orthoptera, Tettigoniidae) in Southern China.

Decma fissa is the most widely distributed species of the genus Decma occuring in southern China. This study presents the first phylogeographic work of D. fissa based on COI, Cytb and ITS sequence. We examined genetic diversity with ITS and mitochondrial sequence respectively, and phylogenetic work was based on the mitochondrial data. A high-level genetic diversity was revealed based on mitochondrial data but a low-level diversity was shown with ITS sequence. For the mitochondrial data, divergence time analysis displayed five lineages. Based on the Mantel test, geographic and genetic distances among D. fissa populations revealed a significant positive correlation. Bayesian skyline plot (BSP) analyses implied that none of three major lineages of D. fissa was seemingly affected by the last glacial maximum (LGM, 0.015-0.025 Mya). Ecological niche modeling was used to predict the distribution of D. fissa in four periods (LGM, Mid-Holocene, current and 2070) in China. Analysis of the ancestral area reconstruction indicated that D. fissa occurred in the South China area.

Review of the genus Woznessenskia Gorochov, 2002 (Orthoptera: Gryllacrididae) from China.

The paper reports four new species of the genus Woznessenskia Gorochov, 2002 and gives supplementary information on Woznessenskia curvicauda(Bey-Bienko, 1962) from China. The specimens examined are deposited in the Museum of Hebei University, Baoding, China.

Further notes on the genus Ocellarnaca Gorochov, 2004 (Orthoptera: Gryllacrididae) from China.

The paper reports three new species of the genus Ocellarnaca Gorochov, 2004 (Orthoptera: Gryllacrididae) from China, i.e. Ocellarnaca brachyptera sp. nov., Ocellarnaca nigrofemora sp. nov., Ocellarnaca nigra sp. nov. The type specimens are deposited in the Museum of Hebei University, Baoding 071002, China.

Review of the genus Diestramima Storozhenko, 1990 (Orthoptera: Rhaphidophoridae: Aemodogryllinae) from China.

In this paper, 6 new species, 1 new subspecies and 1 newly recorded species of the genus Diestramima from China are described, i.e. Diestramima acutiapicis sp. nov., Diestramima bina sp. nov., Diestramima cycla sp. nov., Diestramima truncata sp. nov., Diestramima subtilis sp. nov., Diestramima eurya sp. nov., Diestramima austrosinensis quaterna ssp. nov. and Diestramima major Gorochov, 1998. The morphological photographs of these and 8 known species are provided. All specimens examined are preserved in the Museum of Hebei University.

The genus Mimadiestra Storozhenko Dawwrueng, 2014 (Orthoptera: Rhaphidophoridae) from China, with description of two new species.

In this paper, the genus Mimadiestra Storozhenko Dawwrueng, 2014 is reported from China and two new species are described, i.e. Mimadiestra gracila sp. nov. and Mimadiestra dicha sp. nov. Morphological photographs of the two species are provided. All type specimens are preserved in the Museum of Hebei University.

One new species and one new recorded species of the genus Kuzicus (Tettigoniidae: Meconematinae: Meconematini) from China.

In this paper, a new species of the genus Kuzicus from China, Kuzicus (Kuzicus) leptocercus sp. nov., is described, with a new recorded species, Kuzicus (Kuzicus) koeppeli Sänger & Helfert, 2004 from China. The distribution data and morphological photographs of Kuzicus (Kuzicus) leptocercus sp. nov. and Kuzicus (Kuzicus) koeppeli Sänger & Helfert, 2004 are provided. All specimens are preserved in the Museum of Hebei University.

Expression of potential resistance genes to the English grain aphid, Sitobion avenae, in wheat, Triticum aestivum.

The English grain aphid, Sitobion avenae (F.) (Homoptera: Aphididae), is a dominant and destructive pest in wheat, Triticum estivum L. (Poales: Poaceae), production regions in China and other grain-growing areas worldwide. Patterns of gene expression of the S. avenae-resistant synthetic wheat line 98-10-35, the S. avenae-susceptible line1376, and their hybrid population, and the differences in segments between 98-10-35/1376 F3 resistant plants and resistant parents of 98-10-35, as well as those between the F3 resistant and susceptible populations, were examined with differential display reverse transcription PCR. The results showed that five patterns of differential expression were detected between the progeny and its resistant parents: 1) The gene was silenced in one of the parents; 2) Special expression showed in the progeny; 3) Expression was consistent with the resistant parents; 4) Up expression showed in the progeny but not in the parents; 5) Down expression showed in the progeny but not in the parents. Paired t-test results were not significant; however, the probability value (0.9158) indicated that gene expression on the RNA level were consistent with resistant bands found in F3 resistant individuals and resistant parents, as well as the F3 resistant and susceptible populations. For both the F3 of 98-10-35/1376 and the parents, the total number of amplified bands was 202, with an average of 25.3 per primer. The number of differential bands was 116, with an average of 14.5 per primer amplified and a polymorphism ratio of 56.3%. In the present study, differential expression genes in the resistant line 98-10-35 were all up-regulated. Among them, gene expression of resistant groups in the F3 population was in agreement with patterns 2, 3, and 4. However, the susceptible line 1376 did not have this gene expression on the RNA level. This pattern is expected to be used to select and analyze target genes from the same F3 population and the resistant parents. The results suggest that it can be employed as a new method for molecular assisted breeding.