CDKN2B-AS1 is overexpressed in polycystic ovary syndrome and sponges miR-181a to promote granulosa cell proliferation.

MiR-181a suppresses the proliferation of mouse granulosa cells, which participate in polycystic ovary syndrome (PCOS), suggesting the potential role of miR-181a in PCOS. Our bioinformatics analysis revealed that miR-181a could bind CDKN2B-AS1, a lncRNA regulates ovarian endometriosis. This research was, therefore, conducted to explore the potential crosstalk between CDKN2B-AS1 and miR-181a in PCOS. Expression analysis of CDKN2B-AS1 and miR-181a in follicular fluid from 60 PCOS patients and 60 controls was done with reverse transcriptions-quantitative PCRs. The direct interaction between CDKN2B-AS1 and miR-181a was predicted by IntaRNA and confirmed by RNA pull-down assay. CDKN2B-AS1 in nuclear and cytoplasm of granulosa cells was detected by cellular fractionation assay. The role of CDKN2B-AS1 and miR-181a in granulosa cell proliferation was analyzed by 5-bromodeoxyuridinc assay. In this study, CDKN2B-AS1 was expressed in high amounts in PCOS, whereas miR-181a was downregulated in PCOS, CDKN2B-AS1 was detected in both nucleus and cytoplasm. Although CDKN2B-AS1 and miR-181a were not closely correlated, CDKN2B-AS1 directly interacted with miR-181a. CDKN2B-AS1 and miR-181a overexpression failed to affect the expression of each other. In addition, the inhibitory effect of miR-181a on granulosa cell proliferation was attenuated by CDKN2B-AS1. CDKN2B-AS1 is overexpressed in PCOS and may sponge miR-181a to promote granulosa cell proliferation. Our study characterized a novel CDKN2B-AS1/miR-181a pathway in PCOS. This novel pathway may serve as a potential target to treat PCOS.

Value-based routing of delayed intentions into brain-based versus external memory stores.

Individuals have the option of remembering delayed intentions by storing them in internal memory or offloading them to an external store such as a diary or smartphone alert. How do we route intentions to the appropriate store, and what are the consequences of this? We report three experiments (two preregistered) investigating the role of value. In Experiment 1, participants preferentially offloaded high-value intentions to the external environment. This improved memory for both high- and low-value content. Experiment 2 replicated the low-value memory enhancement even when only high-value intentions were offloaded. This provides evidence for a cognitive spillover effect: When high-value content is offloaded, internal memory becomes reallocated to low-value content instead. Experiment 3 confirmed a theoretical prediction of this account: participants had superior memory for low- than high-value content when the external store was removed. These results imply that value-based offloading can lead to a cognitive spillover effect from high- to low-value content, similar to the automatic allocation of "spare" capacity that has been proposed in the domain of visual attention. Individuals prioritize high-value information for external memory; consequently, they can be left with predominantly low-value information if it fails. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Current status and reflections on fertility preservation in China.

PURPOSE: With the progress of medical technology and renovated conception of fertility, the prospective studies and practice of fertility preservation are drawing more and more attention from medical workers. With the largest population of over 1.4 billion, China makes the experience accumulated in fertility preservation efforts even more relevant. This article summarizes China's experience and shares it with the world to promote the healthy development of fertility preservation. METHODS: This study was based on multiple Chinese expert consensuses on fertility preservation issued in 2021 and the current national regulations and principles, compared with the latest advice and guidelines issued by global reproductive authorities such as the ASRM and ESHRE. Summarize the experience and reflection of Chinese scholars in the process of fertility preservation. RESULTS: This study reports on the current situation of fertility preservation in China, sharing the Chinese experience gained in the process of development, and offering Chinese reflections on worrying issues. CONCLUSION: Fertility preservation is a medical and social issue of reproductive health security, which is conducive to the sound development of the world population and social production.