RESUMO
The capacity of P. mirabilis and P. vulgaris strains isolated in acute enteric infections in children for producing enterohemolysin, a new type of hemolysin, has been shown. The relationship between the capacity of Proteus cultures for producing enterohemolysin and their capacity for inducing toxic secretory reaction on a ligated loop on the small intestine of rabbits in the absence of known thermostable and thermolabile antitoxin in bacteria.
Assuntos
Enterotoxinas/biossíntese , Proteínas Hemolisinas/biossíntese , Enteropatias/microbiologia , Infecções por Proteus/microbiologia , Proteus mirabilis/patogenicidade , Proteus vulgaris/patogenicidade , Doença Aguda , Animais , Animais Lactentes , Pré-Escolar , Diarreia/etiologia , Diarreia/microbiologia , Enterotoxinas/análise , Fezes/microbiologia , Proteínas Hemolisinas/análise , Humanos , Lactente , Enteropatias/etiologia , Camundongos , Infecções por Proteus/etiologia , Proteus mirabilis/isolamento & purificação , Proteus vulgaris/isolamento & purificação , CoelhosRESUMO
The capacity of Proteus strains, isolated from patients with purulent inflammatory, urological and enteric infections, for the production of choleriform thermolabile enterotoxin was studied by means of the enzyme immunoassay (EIA) with the use of antitoxic serum to Escherichia coli enterotoxin. Out of 125 strains, 27 (21.6%) showed the capacity for producing choleriform thermolabile enterotoxin in EIA experiments. The results thus obtained indicate that EIA techniques can be used, in principle, for detecting the capacity of Proteus for the production of choleriform thermolabile enterotoxin.