[Methods of reducing the amount of pediculocide residues on bed linens to safe levels]. / Metody snizheniia ostatochnykh kolichestv pedikulitsidov na be'le do bezopasnykh urovnei.

The means for reducing the residue of organophosphorous and pyrethrin pediculicides on the fabric to safety levels have been developed due to the use of gas and liquid chromatography. The proposed procedures include linen washing after its pediculicidal treatment. Optimal conditions for the removal of the above substances from the fabric have been set forth. Actual quantity of pediculicides on the fabric after its treatment has been identified.

[Isolation and purification of restriction endonuclease SacI from Streptomyces achromogenes ATCC 12767]. / Vydelenie i ochistka éndonukleazy restriktsii SacI iz Streptomyces achromogenes ATCC 12767.

A procedure for isolation and purification of restriction endonuclease Sac I from Streptomyces achromogenes ATCC 12767 is proposed. It allows to obtain an electrophoretically homogeneous enzyme preparation with the purification degree 1097 and the enzyme yield by activity 3.7%. The molecular weight of SacI was found to be 52,000 +/- 5,000 D, and isoelectric point 6.2. The enzyme consists of two subunits, which was found by polyacrylamide gel electrophoresis under denaturing conditions. Km and Vmax values were determined for the enzymatic reaction; they are equal to 4.6 X 10(-9) M and 9.19 X 10(-10) M/min, respectively.

[Bone scintigraphy in the preoperative determination of the stage of breast cancer]. / Osteostsintigrafiia v predoperatsionnom opredelenii stadii raka molochnoi zhelezy.

As for the frequency of metastases to the bones breast cancer ranks third among all tumors, as for the general frequency of metastases it ranks first (25-45%). Early diagnosis of breast cancer metastases to the bones using scintigraphy of the skeleton in the preoperative period gives an opportunity to reveal tumor dissemination and to provide adequate treatment without imposing on patients inappropriate therapeutic measures. The study was performed using 99mTc-pyrophosphate. In revealing the foci of high activity accumulation by more than 20% as compared to symmetrical or adjacent zones, the changes were looked upon as metastatic ones. Out of 85 patients foci of high activity accumulation in the skeleton were noted in 7 (8.3%). In 5 patients, metastases were confirmed directly on roentgenography, and in 2 patients, they manifested themselves 2 and 5 months later. Stage I-IIa disease was observed in 48 (56.5%) out of the 85 patients. Out of the 40 patients with Stage I disease, skeletal metastases confirmed by x-ray were found in 2. All 7 patients were given together with mastectomy adjuvant treatment. Thus scintigraphy of the skeleton together with chest x-ray, liver scintigraphy, physical examination and palpation should become obligatory in the preoperative examination of patients to specify breast cancer stage.