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1.
Artigo em Inglês | MEDLINE | ID: mdl-37102359

RESUMO

Phylogenetic tree analysis of Youhaiella tibetensis F4T, Paradevosia shaoguanensis J5-3T and Methyloterrigena soli M48T based on 16S rRNA gene sequences revealed that the three genera did not form distinct and independent monophyletic clusters. The 16S rRNA gene sequence similarities were all higher than 99 % between each pair of the three type strains. Average nucleotide identity, digital DNA-DNA hybridization, average amino acid identity and 16S rRNA gene sequence similarity between Paradevosia shaoguanensis J5-3T and Methyloterrigena soli M48T showed that they represented the same species. Physiological and biochemical characteristics of these three strains were similar, such as motility by polar flagella, major respiratory quinone, polar lipids, and fatty acids. Polygenetic trees and other characteristic comparative analysis revealed the need to merge the genera Youhaiella and Paradevosia into one genus.


Assuntos
Ácidos Graxos , Ácidos Graxos/química , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , DNA Bacteriano/genética , Técnicas de Tipagem Bacteriana , Composição de Bases , Hibridização de Ácido Nucleico
3.
Arch Microbiol ; 204(7): 390, 2022 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-35699786

RESUMO

Strain WGZ8T was isolated from a soil sample of Puerh tea garden in Pu'er city, Southwest China. The isolate was rod-shaped, Gram-stain negative, facultative anaerobic, non-motile. Growth occurred within 0-3.0% (w/v) NaCl (optimal concentration, 0-1.0%), pH 5.0-11.0 (optimal pH, 7.0) and 10-40 °C (optimal temperature, 28 °C). 16S rRNA gene sequence-based phylogenetic and phylogenomic analysis revealed that WGZ8T belonged to the genus Microvirga. Its major cellular fatty acids were C19:0 cyclo ω8c, C16:0, C18:1ω7c and/or C18:1ω6c. The profile of polar lipids included phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol and phosphatidylglycerol. The only respiratory quinone was detected as ubiquinone 10 (Q-10). The genome size of strain WGZ8T was 5.17 MB, and the content of DNA G + C was 61 mol%. Based on the results of digital DNA-DNA hybridization and phenotypic results, strain WGZ8T could be concluded as a novel species of the genus Microvirga, for which the name Microvirga puerhi sp. nov. is proposed. The type strain is WGZ8T (= CGMCC 1.19171 T = JCM 35317 T).


Assuntos
Bradyrhizobiaceae , Methylobacteriaceae , Técnicas de Tipagem Bacteriana , Composição de Bases , Bradyrhizobiaceae/genética , DNA Bacteriano/genética , Ácidos Graxos/análise , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Solo , Microbiologia do Solo , Chá
4.
Curr Microbiol ; 79(7): 194, 2022 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-35579716

RESUMO

A Gram-positive-staining, strictly aerobic, motile, ellipsoidal endospore-forming bacterial strain, designated CHY01T, was isolated from the Chishui river in a section of Maotai Town, Guizhou Province, Southwest China. Strain CHY01T was found to grow optimally at pH 8.0 and 28 °C. The 16S rRNA gene sequence analysis indicated that strain CHY01T belonged to the genus Brevibacillus and clustered with the type strain of Brevibacillus panacihumi, with which it exhibited 16S rRNA gene sequence similarity values of 97.8%. The predominant respiratory quinone was MK-7, and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The major fatty acids were C14:0, iso-C15:0, anteiso-C15:0, C16:0, C15:1iso-H and/or C13:0 3-OH, and C16:1ω7c and/or C16:1ω6c. Genome sequencing revealed a genome size of 6.1 Mbp and a G + C content of 50.6%. The results of physiological and biochemical tests allowed strain CHY01T to be distinguished genotypically and phenotypically from Brevibacillus species with validly published names. Pairwise determined whole-genome average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values suggested that strain CHY01T represents a new species, for which we propose the name Brevibacillus dissolubilis sp. nov. with the type strain CHY01T (= CGMCC 1.15916 T = KCTC 33863 T).


Assuntos
Brevibacillus , Técnicas de Tipagem Bacteriana , Brevibacillus/genética , DNA Bacteriano/genética , Ácidos Graxos/química , Água Doce , Fosfolipídeos/química , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
5.
Arch Microbiol ; 203(7): 4517-4523, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34146114

RESUMO

A novel Gram-negative, cream-colored, rod-shaped, aerobic, non-motile bacterium, designated MSA67T, was isolated from a subterranean sediment sample of the Mohe Basin in Northeast China. Strain MSA67T was detected to grow at 4-40 °C (optimum 28-30 °C), pH 5.0-10.0 (optimum, pH 7.0) and in 0.0-8.0% (w/v) NaCl (optimum 2.0-3.0%). Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain MSA67T was a member of the genus Devosia, with the highest similarity with D. riboflavina IFO13584T (98.0%) and D. chinhatensis IPL18T (97.0%). The major cellular fatty acids are C16:0, C18:1ω7c 11-methyl and C18:1ω6c and/or C18:1ω7c. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, glycolipids and three unidentified phospholipids. The major respiratory quinone is ubiquinone 10 (Q-10). The genomic size of strain MSA67T is 4.1 MB and DNA G + C content is 63.6%. Based on genotypic, phenotypic and phylogenetic results, strain MSA67T is concluded to represent a novel species of the genus Devosia, for which the name Devosia sediminis sp. nov. is proposed. The type strain is MSA67T (= CGMCC 1.18467T = KCTC 82192T).


Assuntos
Sedimentos Geológicos , Hyphomicrobiaceae , China , Ácidos Graxos/análise , Sedimentos Geológicos/microbiologia , Hyphomicrobiaceae/química , Hyphomicrobiaceae/classificação , Hyphomicrobiaceae/genética , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Especificidade da Espécie , Ubiquinona
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