Investigation of pro- and antioxidative systems' changes in blood of patients with prostate tumours.

Great number of pathogenic processes, including cancer, are followed by disbalance between pro- and antioxidative systems. This is reflected on lipid peroxidation (LP). As a result diene conjugates - the primary products of peroxidation, cetodienes, trienes (secondary products), malondialdehyde (MDA) and Schiff's bases (the final products) are synthesized. Significant changes are expectable in functioning of antioxidant system on the background of tumour growth. The aim of the study was to investigate changes in the content of primary, secondary and final LP products and quantitative changes of main antioxidant of plasma - Ceruloplsmin (Cp) in blood of patients with prostate tumors (benign prostate hyperplasia (BPH), benign prostate hyperplasia with PING(3-4) regions and metastatic prostate adenocarcinoma (PCa). The tendency of diene conjugates reducing has been revealed compared with norm: The control group-->BPH-->benign prostate hyperplasia with PING(3-4)regions-->metastatic PCa. Level of cetodienes and trienes was sharply decreased in case of PCa. MDA content was increased in all cases we had studied, but it was significantly increased in benign prostate hyperplasia with PING(3-4) regions and PCa. Amount of Schiff's bases raised in lipid extracts in all cases of prostate cancer, especially in patients with benign prostate hyperplasia with PING(3-4) regions. Concentration of Cp was sharply increased in blood plasma of patients with malignant tumour. Strong intensification of LP in prostate cancer patients and accumulation of its final products occurs on the background of changes of the antioxidant system. This fact was revealed by sharp increase of Cp content in blood plasma of the patients. Both facts express disbalances of the anti- and prooxidative systems of organism in case of prostate gland malignant transformation.

Activity and content of antioxidant enzymes in prostate tumors.

AIM: To investigate the antioxidant enzyme system in blood of men with benign hyperplasia of prostate (BHP) and with prostate adenocarcinoma (CaP). METHODS: The spectrophotometrical methods were applied to study content and activity changes of superoxide-dismutase (SOD), catalase (CAT), ceruloplasmin (Cp), tripeptide glutathione (GSH), glutathione-peroxidase (GSH-Px), and glutathione-reductase (GR). Lipid peroxidation was evaluated using thiobarbituric acid (TBA)-test. Blood plasma and erythrocytes of men with prostate tumors served as the material for the studies; n = 15 for each group. RESULTS: SOD activity was increased in BHP (24.65 +/- 1.20 U/microl) and decreased in CaP (11.45 +/- 0.89 U/microl), CAT activity remained unaltered in BHP (12.41 +/- 0.85 mcat/ml) and was slightly declined in CaP (9.52 +/- 0.56 mcat/ml). Cp was increased in both kind of tumors, especially in CaP (54.27 +/- 7.22 mg%), as well as GSH (0.736 +/- 0.07 microM/l) and GR (0.031 +/- 0.002 microM/g.Hemogl/min). GSH-Px was sharply increased in BHP (0.67 +/- 0.05 microM/g.Hemogl/min) and reduced in CaP (0.16 +/- 0.01 microM/g.Hemogl/min). CONCLUSION: The development of BHP reflects relatively weakly on blood system as activity and content of antioxidant enzymes are not revealing marked changes in this disease. The significant changes are revealed in case of CaP, showing the reduced functional state of blood antioxidant enzyme system.