Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 110
Filtrar
1.
Hernia ; 23(4): 757-765, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30805828

RESUMO

PURPOSE: Estimation and comparison of results after incisional hernia repair (IHR) modo onlay or sublay with abdominoplasty in patients who lost the weight following Roux-en-Y Gastric Bypass (RYGB). Analysis and comparison of changes in quality of life (QL) of these patients prior to RYGB, before and after simultaneous IHR and abdominoplasty. METHODS: Clinical analysis involved 40 patients with abdominal disfigurement (following RYGB and massive weight loss) after one-time IHR sublay method with abdominoplasty-group 1 or IHR onlay method with abdominoplasty-group 2. We evaluated postoperative results and long-term QL changes (DAS24, SF-36 scales). RESULTS: We noted abnormal wound healing (2), pneumonia (3) and dysesthesia (3) in patients from group 1, and abnormal wound healing (2), seroma (2), pneumonia (2), and dysesthesia (4) in group 2. Quality of life was improved in the functional, esthetic and psychological aspects. CONCLUSIONS: One stage incisional hernia repair by onlay as well as sublay method with abdominoplasty are safe surgical methods improving the functioning of patients after major weight loss following RYGB. Sublay hernia repair and abdominoplasty was connected with longer time of the: operation, drainage, analgesic agents use, time to mobilization and to full oral diet than the onlay method. Significant improvement of the quality of life was noted after every subsequent step of surgical treatment in both groups. Reduction of the risk of BMI re-growth after bariatric surgery is related to the need for constant, specialized care for these patients at every stage of follow-up after bariatric surgery.


Assuntos
Abdominoplastia/métodos , Derivação Gástrica , Hérnia Ventral/cirurgia , Herniorrafia/métodos , Hérnia Incisional/cirurgia , Adulto , Estudos de Coortes , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Período Pós-Operatório , Qualidade de Vida , Seroma/etiologia , Redução de Peso , Adulto Jovem
2.
Transplant Proc ; 48(5): 1767-9, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27496488

RESUMO

BACKGROUND: The long-term survival of 209 consecutive patients (mean age, 46 ± 15 years) from a single center with ≥1 diagnostic myocardial biopsy after heart transplantation was analyzed. METHODS: Patients were considered as C4d positive if a capillary staining (immunohistochemistry in paraffin samples) was observed in ≥1 myocardial biopsy. Data were analyzed according to pathologic consensus of antibody mediated rejection definition of C4d+ positivity: 2004 definition in group A and the 2013 definition in group B and compared with their respective controls, composed of patients who do not meet those criteria. Age, follow-up time, and number of biopsies were comparable between patients with C4d+ and controls in both groups. Follow-up was 100% complete with mean of observation time 2143 days. RESULTS: During the follow-up period, 62 patients died (group A: C4d+ 32% vs controls 29%; group B: C4d+ 36% vs controls 29% [P = NS]). There were no differences in survival between patients with positive staining and without C4d+ staining when Kaplan-Meier survival curves were compared. CONCLUSIONS: The presence of C4d positive staining in myocardial capillaries of heart biopsies of patients after heart transplantation, as an isolated finding, was not related to worse long-term survival.


Assuntos
Capilares/metabolismo , Complemento C4b/metabolismo , Transplante de Coração/mortalidade , Miocárdio/patologia , Biópsia , Feminino , Rejeição de Enxerto/imunologia , Rejeição de Enxerto/mortalidade , Humanos , Imuno-Histoquímica , Estimativa de Kaplan-Meier , Transplante de Rim/mortalidade , Masculino , Pessoa de Meia-Idade , Miocárdio/metabolismo , Estudos Retrospectivos , Coloração e Rotulagem/métodos
3.
Annu Int Conf IEEE Eng Med Biol Soc ; 2015: 735-8, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26736367

RESUMO

This work presents a method for automatical and objective classification of patients with healthy and pathological vocal fold vibration impairments using High-Speed Videoendoscopy of the larynx. We used an image segmentation and extraction of a novel set of numerical parameters describing the spatio-temporal dynamics of vocal folds to classification according to the normal and pathological cases and achieved 73,3% cross-validation classification accuracy. This approach is promising to develop an automatic diagnosis tool of voice disorders.


Assuntos
Voz , Humanos , Laringoscopia , Laringe , Vibração , Gravação em Vídeo , Prega Vocal , Distúrbios da Voz
4.
Transplant Proc ; 43(8): 3082-5, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21996231

RESUMO

OBJECTIVE: The aim of this study was to compare health behaviors among patients after heart transplantation, depending on the mode of qualification for the surgery. PATIENTS AND METHODS: A questionnaire was completed by 115 patients who underwent heart transplantation surgery. The first part covered sociodemographic data before and after transplantation; the second part the medical data, and the last part behaviors before and after transplantation, such as smoking tobacco, drinking alcohol, and systematic compliance to medications. Behaviors associated with health were evaluated with the of a health behavior inventory including 24 statements referring to behaviors associated with health. RESULTS: Fifty-two patients (45.21%) including 13 women and 39 men underwent a heart transplantation performed in the "urgent" mode. They were significantly younger than those with a "planned" transplantation: 50.65±16.19 vs 57.22±10.26 (P<.05). No significant differences were observed in smoking tobacco or drinking alcohol after the transplantation. However, patients transplanted in the "urgent" mode more frequently were compliant to the medication regimen than those in the "planned" mode. These results showed a significant influence of the transplantation mode both on the eating habits and every day health practices after transplantation (P<.05). People awaiting transplantation at home showed a stronger conviction about the influence of coincidental (chance) factors on their health, in opposition to their own behavior or the actions of the medical staff (P<.05). CONCLUSIONS: Patients transplanted in the urgent mode showed a higher level of health practices after transplantation, more frequent attention to their health condition as dependent on their own behavior or the medical crew's actions, and a higher level of health practices compared with other cardiac patients.


Assuntos
Comportamentos Relacionados com a Saúde , Transplante de Coração/psicologia , Adulto , Idoso , Consumo de Bebidas Alcoólicas , Procedimentos Cirúrgicos Eletivos/psicologia , Comportamento Alimentar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Cooperação do Paciente , Polônia , Fumar , Inquéritos e Questionários
5.
Am J Transplant ; 10(7): 1677-85, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20642689

RESUMO

The measurement properties of the newly developed Pediatric Quality of Life Inventory (PedsQL) 3.0 Transplant Module in pediatric solid organ transplant recipients were evaluated. Participants included pediatric recipients of liver, kidney, heart and small bowel transplantation who were cared for at seven medical centers across the United States and their parents. Three hundred and thirty-eight parents of children ages 2-18 and 274 children ages 5-18 completed both the PedsQL 4.0 Generic Core Scales and the Transplant Module. Findings suggest that child self-report and parent proxy-report scales on the Transplant Module demonstrated excellent reliability (total scale score for child self-report alpha= 0.93; total scale score for parent proxy-report alpha= 0.94). Transplant-specific symptoms or problems were significantly correlated with lower generic HRQOL, supporting construct validity. Children with solid organ transplants and their parents reported statistically significant lower generic HRQOL than healthy children. Parent and child reports showed moderate to good agreement across the scales. In conclusion, the PedsQL Transplant Module demonstrated excellent initial feasibility, reliability and construct validity in pediatric patients with solid organ transplants.


Assuntos
Nível de Saúde , Transplante de Órgãos/fisiologia , Qualidade de Vida , Adolescente , Adulto , Criança , Pré-Escolar , Estudos de Viabilidade , Feminino , Humanos , Masculino , Transplante de Órgãos/psicologia , Pais/psicologia , Psicologia da Criança , Reprodutibilidade dos Testes , Estados Unidos
6.
Transplant Proc ; 41(8): 3161-5, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19857702

RESUMO

UNLABELLED: Most methods used in the risk assessment of heart transplant candidates do not include new biomarkers. AIMS: The aim of the study was to examine the value of NTproBNP and hsCRP and their combined use together with HFSS score in the risk assessment of patients with heart failure enlisted for heart transplantation. METHODS: Data of 658 patients enlisted for heart transplantation in all active transplantation centers were stored in a prospective registry. The composite end point-death or urgent transplantation was recorded during the follow-up. RESULTS: Death or urgent transplantation was recorded in 161 (24%) of pts. 102 (15%) patients died and 59 (9%) were transplanted urgently. Kaplan-Meier curves for risk of death or urgent transplantation where highly significant when pts were stratified by the quartiles of NT proBNP (P < .000001) or quartiles according to the hsCRP level on admission (P < .002). In the multivariate Cox proportional hazard model, the significance was observed for NTproBNP (P < .01) and HFSS (P < .02), and borderline significance for hsCRP (P = .057). When ROC analyses of the area under the curve (AUC) values were considered, AUC area was for HFSS - 0.645, for NTproBNP - 0.653 and for hsCRP - 0.566. When all those variables were included together in the model, the AUC value rose to 0.6943. Based on those results a weighted risk model with all three parameters was proposed. CONCLUSION: HFSS, NTproBNP and hsCRP levels are independent stratification variables of survival or need for urgent heart transplantation. Their predictive value is moderately increased when they are analyzed together.


Assuntos
Insuficiência Cardíaca/cirurgia , Transplante de Coração/fisiologia , Medição de Risco , Listas de Espera , Proteína C-Reativa/metabolismo , Insuficiência Cardíaca/fisiopatologia , Transplante de Coração/mortalidade , Humanos , Estudos Multicêntricos como Assunto , Peptídeo Natriurético Encefálico/sangue , Fragmentos de Peptídeos/sangue , Polônia , Valor Preditivo dos Testes , Probabilidade , Sistema de Registros , Fatores de Risco , Análise de Sobrevida , Sobreviventes
7.
Transplant Proc ; 41(8): 3166-70, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19857703

RESUMO

INTRODUCTION: The aim of the study was to analyze the influence of body weight of the adult heart recipient on the chance to obtain a transplant. METHODS: We analyzed the data from all 658 patients listed for heart transplantation. RESULTS: During the follow-up period, 325 (49%) of listed patients underwent transplantation with 102 (15%) succumbing before heart transplantation. The mean weight of transplanted patients was 73.7 +/- 13.7 kg and 81.2 +/- 15.4 kg for those not transplanted (P < .00001). Patients were divided according to body weight in two groups: light = below 80 kg (n = 360) or heavy > or = 80 kg or above (n = 297). On the transplant list, 111 heavy patients (37%) versus 213 light patients (59%) underwent the procedure, a significant difference. The waiting time among light patients was 255 versus heavy patients of 395 days (P < .005). There was a similar number of deaths before transplantation among the light (n = 56 360 patients; 15.5%) versus the heavy group (49/297; 16%). Upon multivariate Cox mode analysis independent factors related to not receiving a heart transplant were greater weight, systolic blood pressure, pulmonary vascular resistance, Heart Failure Survival Score (HFSS) score and lower N-terminal pro-brain natriuretic peptide (NTproBNP) levels. CONCLUSIONS: Among adult heart transplant candidates, the chance to receive a heart transplant significantly decreased when the recipient's weight exceeded 80 kg. Patients with a body weight more than 110 kg had a poor chance to receive a heart transplantation.


Assuntos
Peso Corporal , Insuficiência Cardíaca/fisiopatologia , Transplante de Coração/estatística & dados numéricos , Adulto , Pressão Sanguínea , Estatura , Diástole , Insuficiência Cardíaca/mortalidade , Insuficiência Cardíaca/cirurgia , Frequência Cardíaca , Transplante de Coração/mortalidade , Transplante de Coração/fisiologia , Humanos , Pessoa de Meia-Idade , Peptídeo Natriurético Encefálico/sangue , Sobrepeso/epidemiologia , Fragmentos de Peptídeos/sangue , Polônia , Probabilidade , Sistema de Registros , Taxa de Sobrevida , Sobreviventes , Magreza/epidemiologia , Resistência Vascular , Função Ventricular Esquerda
8.
Artigo em Inglês | MEDLINE | ID: mdl-18002580

RESUMO

The paper addresses data processing support that is required in capsule gastrointestinal endoscopy. First, capsule position estimation method using standard MPEG-7 image features (descriptors) is discussed. The proposed approach makes use of vector quantization, principal component analysis and neural networks. Next, new algorithms dedicated for virtual colonoscopy (VC) human body inspection are described. The VC images can be registered with endoscopic ones and help in capsule localization and navigation. Finally, an original, low-complexity, efficient image compression method, based on integer-to-integer 4x4 DCT transform, is presented and experimentally verified.


Assuntos
Endoscopia por Cápsula/métodos , Trato Gastrointestinal/anatomia & histologia , Processamento de Imagem Assistida por Computador , Algoritmos , Colo/anatomia & histologia , Colo/diagnóstico por imagem , Colonografia Tomográfica Computadorizada , Compressão de Dados , Gastroscopia/métodos , Humanos , Estômago/anatomia & histologia , Estômago/diagnóstico por imagem
9.
Int J Comput Dent ; 7(3): 253-62, 2004 Jul.
Artigo em Inglês, Alemão | MEDLINE | ID: mdl-15756947

RESUMO

The demonstration of patient case reports in the course of a clinical round is an essential part of teaching medicine and dentistry. However, suitable live patients with particular problems are not always available at a time when teaching is taking place. This project therefore had the objective of establishing a web-based, virtual e-learning concept for demonstrating case reports independent of time and place, with the possibility of an interactive examination, diagnosis, and interdisciplinary therapy decision making for medical and dental students. Anonymized case reports of diseases in the oral and maxillofacial region and the interdisciplinary treatment were digitized and prepared in a web-based format. The technical aspect was based on connecting flash modules with videos and animation, and monitoring through HTML and Javascript. Due to the modular concept and the programming used, the learning environment was independent of platform and open. Independent formats (.swf, .avi, .mpeg, etc.) were integrated into the individual modules. According to a hierarchic decision system, the user was guided interactively to the diagnosis through a differential diagnostic exclusion process. Sound was digitized and integrated in mp3 compressed form in the 3D models for lip-synchronous speech output. The speech output was connected with a virtual 3D tutor that acted in an advisory capacity in reaching a diagnosis and determining therapy. Further sources of information and literature with abstracts or pdf files of the subject-related publications were inserted to ensure that the teaching was objective. To conclude the virtual clinical round, a check on learning success was conducted in the form of a multimedia multiple choice test.


Assuntos
Instrução por Computador , Educação Continuada em Odontologia/métodos , Doenças da Boca/diagnóstico , Diagnóstico Diferencial , Educação a Distância , Avaliação Educacional , Humanos , Imageamento Tridimensional , Doenças da Boca/terapia , Multimídia , Equipe de Assistência ao Paciente , Interface Usuário-Computador
10.
Crit Care Med ; 29(8): 1587-92, 2001 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-11505132

RESUMO

OBJECTIVE: The intraosseous route is an emergency alternative for the administration of drugs and fluids if vascular access cannot be established. However, in hemorrhagic shock or after vasopressors are given during resuscitation, bone marrow blood flow may be decreased, thus impairing absorption of intraosseously administered drugs. In this study, we evaluated the effects of vasopressin vs. high-dose epinephrine in hemorrhagic shock and cardiac arrest on bone marrow blood flow. DESIGN: Prospective, randomized laboratory investigation that used an established porcine model for measurement of hemodynamic variables and organ blood flow. SETTING: University hospital laboratory. SUBJECTS: Fourteen pigs weighing 30 +/- 3 kg. INTERVENTIONS: Radiolabeled microspheres were injected to measure bone marrow blood flow during a prearrest control period and during hypovolemic shock produced by rapid hemorrhage of 35% of the estimated blood volume. In the second part of the study, ventricular fibrillation was induced; after 4 mins of untreated cardiac arrest and 4 mins of standard cardiopulmonary resuscitation, a bolus dose of either 200 microg/kg epinephrine (n = 6) or 0.8 units/kg vasopressin (n = 6) was administered. Defibrillation was attempted 2.5 mins after drug administration, and blood flow was assessed again at 5 and 30 mins after successful resuscitation. MEASUREMENTS AND MAIN RESULTS: Mean +/- sem bone marrow blood flow decreased significantly during induction of hemorrhagic shock from 14.4 +/- 4.1 to 3.7 +/- 1.8 mL.100 g-1.min-1 in the vasopressin group and from 18.2 +/- 4.0 to 5.2 +/- 1.0 mL.100 g-1.min-1 in the epinephrine group (p =.025 in both groups). Five minutes after return of spontaneous circulation, mean +/- sem bone marrow blood flow was 3.4 +/- 1.1 mL.100 g-1.min-1 after vasopressin and 0.1 +/- 0.03 mL.100 g-1.min-1 after epinephrine (p =.004 for vasopressin vs. epinephrine). At the same time, bone vascular resistance was significantly (p =.004) higher in the epinephrine group when compared with vasopressin (1455 +/- 392 vs. 43 +/- 19 mm Hg. mL-1.100 g.min, respectively). CONCLUSIONS: Bone blood flow responds actively to both the physiologic stress response of hemorrhagic shock and vasopressors given during resuscitation after hypovolemic cardiac arrest. In this regard, bone marrow blood flow after successful resuscitation was nearly absent after high-dose epinephrine but was maintained after high-dose vasopressin. These findings emphasize the need for pressurized intraosseous infusion techniques, because bone marrow blood flow may not be predictable during hemorrhagic shock and drug therapy.


Assuntos
Medula Óssea/irrigação sanguínea , Epinefrina/uso terapêutico , Parada Cardíaca/etiologia , Choque/tratamento farmacológico , Vasoconstritores/uso terapêutico , Vasopressinas/uso terapêutico , Animais , Medula Óssea/efeitos dos fármacos , Feminino , Hemodinâmica , Infusões Intraósseas , Microesferas , Ressuscitação , Choque/complicações , Suínos
11.
J Heart Valve Dis ; 10(4): 545-7, 2001 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-11499605

RESUMO

A 30-year old man underwent a head-on motor accident and was admitted to hospital, where he was successfully treated for several injuries. He was discharged home with slight tricuspid regurgitation, without signs of hemodynamic decompensation, and required no further treatment. Five years later he was admitted to the National Institute of Cardiology due to overt right ventricular failure secondary to tricuspid valve regurgitation. After several unsuccessful attempts at papillary muscle and chordal repair, a 33-mm SJM Biocor valve was implanted. This case history underlines the value of echocardiography, even after blunt chest trauma without cardiac symptoms. A good functional result can be achieved years after onset of traumatic valve insufficiency, but early diagnosis and surgical treatment increases the feasibility of tricuspid valve repair rather than replacement, and also prevents progressive deterioration of right ventricular function.


Assuntos
Acidentes de Trânsito , Insuficiência da Valva Tricúspide/etiologia , Adulto , Humanos , Masculino , Motocicletas , Fatores de Tempo , Insuficiência da Valva Tricúspide/diagnóstico por imagem , Insuficiência da Valva Tricúspide/cirurgia , Ultrassonografia
12.
Cell Biol Toxicol ; 17(2): 117-25, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11499695

RESUMO

Resveratrol (3,4',5-trihydroxystilbene) is a natural molecule with antioxidant action. It is also considered to be a molecule with antiplatelet, anticancer and anti-inflammatory action. The effects of trans-resveratrol on the reactive oxygen species (ROS) generation and thiobarbituric acid-reactive substances (TBARS) in blood platelets induced by endotoxin (lipopolysaccharide, LPS) or thrombin were studied in vitro. The production of superoxide radicals (O2.-) and other reactive oxygen species (H2O2, singlet oxygen, and organic radicals) in the presence of resveratrol was measured by a chemiluminescence method in resting blood platelets and platelets stimulated by LPS (0.3 microg/ 10(8) platelets) or thrombin (2.5 U/10(8) platelets). We have shown that resveratrol (6.25-100 microg/ml) inhibits chemiluminescence and generation of O2.- in blood platelets. It has an inhibitory effect on the production of ROS and TBARS in platelets caused by LPS or thrombin.


Assuntos
Antioxidantes/farmacologia , Plaquetas/efeitos dos fármacos , Lipopolissacarídeos/toxicidade , Espécies Reativas de Oxigênio/metabolismo , Estilbenos/farmacologia , Animais , Plaquetas/metabolismo , Endotoxinas/toxicidade , Hemostáticos/farmacologia , Medições Luminescentes , Análise de Regressão , Resveratrol , Estilbenos/química , Superóxidos/metabolismo , Suínos , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Trombina/farmacologia
13.
Anesth Analg ; 93(3): 649-55, 2001 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-11524335

RESUMO

To improve the efficiency of standard cardiopulmonary resuscitation (CPR), we evaluated the potential value of impeding respiratory gas exchange selectively during the decompression phase of standard CPR in a porcine model of ventricular fibrillation. After 6 min of untreated cardiac arrest, anesthetized farm pigs weighing 30 kg were randomized to be treated with either standard CPR with a sham valve (n = 11) or standard CPR plus a functional inspiratory impedance threshold valve (ITV(TM)) (n = 11). Coronary perfusion pressure (CPP) (diastolic aortic minus right atrial pressure) was the primary endpoint. Vital organ blood flow was assessed with radiolabeled microspheres after 6 min of CPR, and defibrillation was attempted 11 min after starting CPR. After 2 min of CPR, mean +/- SEM CPP was 14 +/- 2 mm Hg with the sham valve versus 20 +/- 2 mm Hg in the ITV group (P < 0.006). Significantly higher CPPs were maintained throughout the study when the ITV was used. After 6 min of CPR, mean +/- SEM left ventricular and global cerebral blood flows were 0.10 +/- 0.03 and 0.19 +/- 0.03 mL. min(-1). g(-1) in the Control group versus 0.19 +/- 0.03 and 0.26 +/- 0.03 mL. min(-1). g(-1) in the ITV group, respectively (P < 0.05). Fifteen minutes after successful defibrillation, 2 of 11 animals were alive in the Control group versus 6 of 11 in the ITV group (not significant). In conclusion, use of an inspiratory impedance valve during standard CPR resulted in a marked increase in CPP and vital organ blood flow after 6 min of cardiac arrest.


Assuntos
Reanimação Cardiopulmonar/instrumentação , Parada Cardíaca/terapia , Respiração Artificial/instrumentação , Animais , Gasometria , Hemodinâmica/fisiologia , Fluxo Sanguíneo Regional/fisiologia , Mecânica Respiratória/fisiologia , Suínos
14.
Thromb Res ; 103(2): 149-55, 2001 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-11457473

RESUMO

Lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria may activate blood platelets. The aim of our study was to evaluate the effects of different forms of Proteus mirabilis LPS and isolated lipid A and polysaccharide part on the production of superoxide radicals in blood platelets and to estimate the role staurosporin, wortmannin and indomethacin on this process. We compared the generation of superoxide radicals in platelets treated with LPS after preincubation with inhibitors of the signal transduction pathways, namely staurosporin (inhibitor of protein kinase C), wortmannin (inhibitor of phosphoinositide 3-kinase), and indomethacin (inhibitor of cycloxygenase). Our results demonstrate that all LPS molecules and their fragments caused a stimulation of O2- generation in platelets (P<.5). LPSS1959 had the strongest stimulatory effect. Straurosporin and wortmannin, but not indomethacin inhibited O2- production in LPS-stimulated platelets. Staurosporin (8 nM) and wortmannin (50 nM) caused about 50% inhibition of thrombin-induced O2- generation in platelets, while indomethacin (10 microM) had only a slight inhibitory effect on this process. Our results provide support that in LPS- and thrombin-activated platelets, at least part of O2- generation in platelets, while indomethacin (10 microM) had only a slight inhibitory effect on this process. Our results provide support that in LPS- and thrombin-activated platelets, at least part of O2- is generated due to the activation of the enzymes (protein kinase C and phosphoinositide 3-kinase) involved in signal transduction pathway. Cycloxygenase seems to be not involved in this process.


Assuntos
Androstadienos/farmacologia , Plaquetas/efeitos dos fármacos , Inibidores de Ciclo-Oxigenase/farmacologia , Inibidores Enzimáticos/farmacologia , Indometacina/farmacologia , Lipopolissacarídeos/farmacologia , Proteus mirabilis/química , Estaurosporina/farmacologia , Superóxidos/metabolismo , Animais , Plaquetas/metabolismo , Ativação Enzimática/efeitos dos fármacos , Inibidores de Fosfoinositídeo-3 Quinase , Proteína Quinase C/antagonistas & inibidores , Transdução de Sinais/efeitos dos fármacos , Suínos , Wortmanina
15.
J Agric Food Chem ; 49(6): 3017-23, 2001 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-11410003

RESUMO

Monoclonal antibodies (MAb) were produced to hexanal-bovine serum albumin conjugates. An indirect competitive ELISA was developed with a detection range of 1-50 ng of hexanal/mL. Hexanal conjugated to three different proteins was recognized, whereas free hexanal and the native proteins were not detected. The antibody cross-reacted with pentanal, heptanal, and 2-trans-hexenal conjugated to chicken serum albumin (CSA) with cross-reactivities of 37.9, 76.6, and 45.0%, respectively. There was no cross-reactivity with propanal, butanal, octanal, and nonanal conjugated to CSA. The hexanal content of a meat model system was determined using MAb and polyclonal antibody-based ELISAs and compared with analysis by a dynamic headspace gas chromatographic (HS-GC) method and a thiobarbituric acid reactive substances (TBARS) assay. Both ELISAs showed strong correlations with the HS-GC and TBARS methods. ELISAs may be a fast and simple alternative to GC for monitoring lipid oxidation in meat.


Assuntos
Aldeídos/isolamento & purificação , Ensaio de Imunoadsorção Enzimática/métodos , Animais , Anticorpos Monoclonais , Cromatografia Gasosa , Reações Cruzadas , Metabolismo dos Lipídeos , Carne/análise , Oxirredução , Substâncias Reativas com Ácido Tiobarbitúrico/análise
16.
Pol Merkur Lekarski ; 10(55): 27-30, 2001 Jan.
Artigo em Polonês | MEDLINE | ID: mdl-11320547

RESUMO

In the years 1983-1997, 252 female to male transsexuals were treated in the Department of Plastic Surgery of University Medical School in Lódz. In 209 patients surgical penis construction was performed. The paper presents operative techniques and results of penis construction with the use of four kinds of flaps: a bipedicled abdominal tube flap, a single pedicled infraumblical flap, a pedicled myocutaneous flap with gracilis muscle and a pedicled lateral groin flap. Overall estimation of early results of penis construction with the use of the four methods applied in the Department showed that we obtained good results in 69.8% of operated transsexuals, satisfactory effects in 24.4% of patients and poor outcome in 5.8% of cases. The best results were achieved with the technique involving a lateral groin flap.


Assuntos
Prótese de Pênis , Retalhos Cirúrgicos , Transexualidade/cirurgia , Adulto , Feminino , Humanos , Pessoa de Meia-Idade
17.
Anesth Analg ; 92(4): 967-74, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11273935

RESUMO

UNLABELLED: The use of an inspiratory impedance threshold valve (ITV) during active compression-decompression (ACD) cardiopulmonary resuscitation (CPR) improves perfusion pressures, and vital organ blood flow. We evaluated the effects of positive end-expiratory pressure (PEEP) on gas exchange, and coronary perfusion pressure gradients during ACD + ITV CPR in a porcine cardiac arrest model. All animals received pure oxygen intermittent positive pressure ventilation (IPPV) at a 5:1 compression-ventilation ratio during ACD + ITV CPR. After 8 min, pigs were randomized to further IPPV alone (n = 8), or IPPV with increasing levels of PEEP (n = 8) of 2.5, 5.0, 7.5, and 10 cm H(2)O for 4 consecutive min each, respectively. Mean +/- SEM arterial oxygen partial pressure decreased in the IPPV group from 150 +/- 30 at baseline after 8 min of CPR to 110 +/- 25 torr at 24 min, but increased in the PEEP group from 115 +/- 15 to 170 +/- 25 torr with increasing levels of PEEP (P <0.02 for comparisons within groups). Mean +/- SEM diastolic aortic minus diastolic left ventricular pressure gradient was significantly (P < 0.001) higher after the administration of PEEP (24 +/- 0 vs 17 +/- 1 mm Hg with 5 cm H(2)O of PEEP, and 26 +/- 0 vs 17 +/- 1 mm Hg with 10 cm H(2)O of PEEP), whereas the diastolic aortic minus right atrial pressure gradient (coronary perfusion pressure) was comparable between groups. Furthermore, systolic aortic pressures were significantly (P < 0.05) higher with 10 cm H(2)O of PEEP when compared with IPPV alone (68 +/- 0 vs 59 +/- 2 mm Hg). In conclusion, when CPR was performed with devices designed to improve venous return to the chest, increasing PEEP levels improved oxygenation. Moreover, PEEP significantly increased the diastolic aortic minus left ventricular gradient and did not affect the decompression phase aortic minus right atrial pressure gradient. These data suggest that PEEP reduces alveolar collapse during ACD + ITV CPR, thus leading to an increase in indirect myocardial compression. IMPLICATIONS: Inspiratory impedance during active compression-decompression cardiopulmonary resuscitation improves perfusion pressures, and vital organ blood flow during cardiac arrest. Increasing levels of positive end-expiratory pressure during performance of active compression-decompression cardiopulmonary resuscitation with an inspiratory impedance valve improves oxygenation, and increases the diastolic aortic-left ventricular pressure gradient and systolic arterial blood pressure.


Assuntos
Reanimação Cardiopulmonar/instrumentação , Descompressão/instrumentação , Respiração com Pressão Positiva/instrumentação , Pressão do Ar , Animais , Débito Cardíaco/fisiologia , Circulação Coronária/fisiologia , Feminino , Alvéolos Pulmonares/fisiologia , Circulação Pulmonar/fisiologia , Troca Gasosa Pulmonar/fisiologia , Suínos , Volume de Ventilação Pulmonar/fisiologia , Fibrilação Ventricular/fisiopatologia
18.
Platelets ; 12(8): 470-5, 2001 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11798396

RESUMO

Adhesion of blood platelets to collagen may be involved in pathogenesis of septic shock after damage of endothelial cells by LPS or inflammatory cytokines. Therefore, analysis of platelet adhesion in the presence of endotoxin seems to be of great importance. We studied in vitro the effects of LPSs (S1959, R110, R45) from Proteus mirabilis (smooth and rough types differing significantly in their composition) on the adhesion of unstimulated and thrombin-stimulated platelets to collagen. The adhesion was measured by a static method as absorbance of cell attached proteins. In this work we report that adhesion of resting platelets to collagen was stimulated by all tested LPSs. The effects were dependent on the doses of LPSs. In the presence of LPSs the inhibition of adhesion of thrombin-treated platelets to collagen was observed. The results presented in this paper indicate that LPSs from Proteus mirabilis may act directly on blood platelets and stimulate adhesion of resting platelets to collagen. On the other hand, LPSs can have an inhibitory effect on adhesion of thrombin-stimulated platelets to collagen.


Assuntos
Plaquetas/efeitos dos fármacos , Colágeno/metabolismo , Lipopolissacarídeos/farmacologia , Trombina/farmacologia , Animais , Ativação Plaquetária , Adesividade Plaquetária , Proteus mirabilis/química
19.
Crit Care Med ; 28(11 Suppl): N207-9, 2000 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11098948

RESUMO

In an effort to improve the efficiency of cardiopulmonary resuscitation (CPR), a new inspiratory impedance threshold valve has been developed to enhance the return of blood to the thorax during the chest decompression phase. This new device enhances negative intrathoracic pressure during chest wall recoil or the decompression phase, leading to improved vital organ perfusion during both standard CPR and active compression-decompression CPR. With active compression-decompression CPR, addition of the impedance threshold valve results in sustained diastolic pressures of >55 mm Hg in patients in cardiac arrest. The new valve shows promise for patients in asystole or shock refractory ventricular fibrillation, when enhanced return of blood flow to the chest is needed to "prime the pump." The potential long-term benefits of this new valve remain under study.


Assuntos
Resistência das Vias Respiratórias , Reanimação Cardiopulmonar/instrumentação , Animais , Modelos Animais de Doenças , Desenho de Equipamento , Hemodinâmica , Humanos , Máscaras , Suínos , Tórax/irrigação sanguínea , Fibrilação Ventricular/terapia
20.
Ginekol Pol ; 71(8): 906-10, 2000 Aug.
Artigo em Polonês | MEDLINE | ID: mdl-11082946

RESUMO

Estimation of umbilical cord blood gasometry is one of the test employed to postnatal state of newborns. This easy investigation can present the grade of newborns' blood oxygenation, which correlate with maternal lungs' ventilation. Because the spirometry parameters are more convenience in vertical position.


Assuntos
Sangue Fetal/metabolismo , Trabalho de Parto/fisiologia , Postura/fisiologia , Adulto , Gasometria , Feminino , Humanos , Consumo de Oxigênio , Gravidez , Espirometria/métodos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...