RESUMO
Lipopolysaccharide (LPS) and its structural components: lipid A, O-specific polysaccharide (O-PS) and oligosaccharide core (OG-core) have been isolated from Cytophaga lytica. 3-Oxytetradecanoic (40.8%) and dodecanoic (28.7%) are the predominant fatty acids of lipid A; pentadecanoic (6.8%), 3-oxyhexadecanoic (6.5%) as well as hexadecanoic (5.4%) acids have been found as well. The content of the rest of fatty acids is inconsiderable (2.3 to 0.5%). OG-core contained monosaccharides both typical of the most Gram-negative bacteria (glucose, mannose, galactose, rhamnose, glucosamine) and rarely occurring one-arabinose. O-PS is represented by glucose, mannose, rhamnose, glucosamine, galactosamine as well as unidentified hexosamine.
Assuntos
Cytophaga/química , Bactérias Gram-Negativas/química , Lipopolissacarídeos/análise , Aminoácidos/análise , Animais , Divisão Celular/efeitos dos fármacos , Chlorocebus aethiops , Cromatografia Gasosa , Ácidos Graxos/análise , Lipídeo A/análise , Lipopolissacarídeos/toxicidade , Camundongos , Monossacarídeos/análise , Antígenos O/análise , Testes de Toxicidade Aguda , Células VeroRESUMO
The experiments on mice and cell culture of a kidney of green monkey Vero have shown that lipopolysaccharides (LPS) of the studied strains 81 and 92 of Cytophaga sp. were nontoxic. They were less active as compared to LPS of other Gram-negative bacteria as to interferon-inducing activity. A comparative study of initial and dephosphorylated LPS has shown that phosphate groups were not responsible for the interferon-inducing activity of LPS of Cytophaga genus representatives.