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1.
J Bacteriol ; 192(14): 3773-9, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20494988

RESUMO

Lipoprotein T (LppT), a membrane-located 105-kDa lipoprotein of Mycoplasma conjunctivae, the etiological agent of infectious keratoconjunctivitis (IKC) of domestic sheep and wild Caprinae, was characterized. LppT was shown to promote cell attachment to LSM 192 primary lamb joint synovial cells. Adhesion of M. conjunctivae to LSM 192 cells is inhibited by antibodies directed against LppT. The RGD (Arg-Gly-Asp) motif of LppT was found to be a specific site for binding of M. conjunctivae to these eukaryotic host cells. Recombinant LppT fixed to polymethylmethacrylate slides binds LSM 192 cells, whereas LppT lacking the RGD site is deprived of binding capacity to LSM 192, and LppT containing RGE rather than RGD shows reduced binding. Synthetic nonapeptides derived from LppT containing RGD competitively inhibit binding of LSM 192 cells to LppT-coated slides, whereas nonapeptides containing RAD rather than RGD do not inhibit. RGD-containing, LppT-derived nonapeptides are able to directly inhibit binding of M. conjunctivae to LSM 192 cells by competitive inhibition, whereas the analogous nonapeptide containing RAD rather than RGD or the fibronectin-derived RGD hexapeptide has no inhibitory effect. These results reveal LppT as the first candidate of a RGD lectin in Mycoplasma species that is assumed to bind to beta integrins.


Assuntos
Aderência Bacteriana/fisiologia , Lipoproteínas/química , Lipoproteínas/metabolismo , Mycoplasma conjunctivae/fisiologia , Membrana Sinovial/citologia , Motivos de Aminoácidos/fisiologia , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Sítios de Ligação , Linhagem Celular , Clonagem Molecular , Regulação Bacteriana da Expressão Gênica/fisiologia , Lipoproteínas/genética , Ovinos
2.
J Microbiol Methods ; 70(2): 384-6, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17570547

RESUMO

Infectious keratoconjunctivitis (IKC), caused by Mycoplasma conjunctivae, is a highly contagious ocular disease in Caprinae. To detect rapidly and sensitively M. conjunctivae from individual conjunctival swabs of infected domestic and wild animals, a specific real-time PCR was developed using an lppS-directed hydrolysis probe in a TaqMan platform.


Assuntos
Doenças das Cabras/diagnóstico , Infecções por Mycoplasma/veterinária , Mycoplasma conjunctivae/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Animais , Animais Domésticos/microbiologia , Animais Selvagens/microbiologia , Cabras , Ceratoconjuntivite Infecciosa/diagnóstico , Infecções por Mycoplasma/diagnóstico , Mycoplasma conjunctivae/genética
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