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Dev Dyn ; 243(12): 1606-18, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25258326

RESUMO

BACKGROUND: Specific molecules involved in early inductive signaling from anterior neural tissue to the placodal ectoderm to establish a lens-forming bias, as well as their regulatory factors, remain largely unknown. In this study, we sought to identify and characterize these molecules. RESULTS: Using an expression cloning strategy to isolate genes with lens-inducing activity, we identified the transcriptional cofactor ldb1. This, together with evidence for its nuclear dependence, suggests its role as a regulatory factor, not a direct signaling molecule. We propose that ldb1 mediates induction of early lens genes in our functional assay by transcriptional activation of lens-inducing signals. Gain-of-function assays demonstrate that the inductive activity of the anterior neural plate on head ectodermal structures can be augmented by ldb1. Loss-of-function assays show that knockdown of ldb1 leads to decreased expression of early lens and retinal markers and subsequently to defects in eye development. CONCLUSIONS: The functional cloning, expression pattern, overexpression, and knockdown data show that an ldb1-regulated mechanism acts as an early signal for Xenopus lens induction.


Assuntos
Proteínas de Ligação a DNA/biossíntese , Ectoderma/embriologia , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Cápsula do Cristalino/embriologia , Organogênese/fisiologia , Proteínas de Xenopus/biossíntese , Animais , Proteínas de Ligação a DNA/genética , Ectoderma/citologia , Embrião não Mamífero/citologia , Embrião não Mamífero/embriologia , Cápsula do Cristalino/citologia , Crista Neural/citologia , Crista Neural/embriologia , Retina/citologia , Retina/embriologia , Proteínas de Xenopus/genética , Xenopus laevis
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