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Mol Cell Biol ; 30(14): 3412-20, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20498279

RESUMO

Adipogenesis is governed by a well-documented cascade of transcription factors. However, less is known about non-transcription factors that govern early stages of adipogenesis. Here we show that cellular retinol-binding protein type I (CRBP-I), a small cytosolic binding protein for retinol and retinaldehyde, is specifically restricted to preadipocytes in white adipose tissue. The absence of CRBP-I in mice (CRBP-I-KO mice) leads to increased adiposity. Despite increased adiposity, CRBP-I-KO mice remain more glucose tolerant and insulin sensitive during high-fat-diet feeding. 3T3-L1 cells deficient in CRBP-I or mouse embryonic fibroblasts derived from CRBP-I-KO mice had increased adipocyte differentiation and triglyceride (TG) accumulation. This was due to increased expression and activity of PPAR gamma, while other transcription factor pathways in early and late differentiation remained unchanged. Conversely, the overexpression of CRBP-I in 3T3-L1 cells results in decreased TG accumulation. In conclusion, CRBP-I is a cytosolic protein specifically expressed in preadipocytes that regulates adipocyte differentiation in part by affecting PPAR gamma activity.


Assuntos
Adipogenia/fisiologia , Proteínas Celulares de Ligação ao Retinol/fisiologia , Células 3T3-L1 , Adipócitos/citologia , Adipócitos/metabolismo , Adiposidade , Animais , Sequência de Bases , Diferenciação Celular/fisiologia , Células-Tronco Embrionárias/citologia , Células-Tronco Embrionárias/metabolismo , Teste de Tolerância a Glucose , Resistência à Insulina , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Obesidade/metabolismo , Obesidade/patologia , PPAR gama/metabolismo , Fenótipo , RNA Interferente Pequeno/genética , Proteínas Celulares de Ligação ao Retinol/antagonistas & inibidores , Proteínas Celulares de Ligação ao Retinol/deficiência , Proteínas Celulares de Ligação ao Retinol/genética , Triglicerídeos/metabolismo
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