Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Eur J Histochem ; 59(1): 2455, 2015 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-25820558

RESUMO

Osteonecrosis of the jaw (ONJ) is a chronic complication affecting long-term bisphosphonate-treated subjects, recognized by non-healing exposed bone in the maxillofacial region. The pathophysiological mechanism underlying ONJ has not been fully elucidated. The aim of the present study was to investigate the role of RANK/RANKL/OPG signaling pathway and, in parallel, to evaluate angiogenic and matrix mineralization processes in jaw bone necrotic samples obtained from bisphosphonate-treated subjects with established ONJ. Necrotic bone samples and native bone samples were processed for Light and Field Emission in Lens Scanning Electron Microscope (FEISEM) analyses, for Real-Time RT-PCR to evaluate the gene expression of TNFRSF11A (RANK), TNFSF11 (RANKL), and TNFSF11B (OPG) and for immunohistochemical analyses of VEGF and BSP expression. Morphological analyses performed by Light microscope and FEISEM show empty osteocytic lacunae and alteration of lamellar organization with degradation of the mineralized bone matrix in necrotic bone samples. A significant increase in TNFRSF11A, TNFSF11, TRAF6 and NFAT2 gene expression, and a reduction of TNFSF11B gene transcription level compared is also showed in necrotic bone compared to control samples. No significant difference of VEGF expression is evidenced, while lower BSP expression in necrotic bone compared to healthy samples is found. Even if the pathogenesis of bisphosphonate-associated ONJ remains unknown, a link between oral pathogens and its development seems to exist. We suppose lipopolysaccharide produced by bacteria colonizing and infecting necrotic bone and the surrounding viable area could trigger RANK/RANKL/OPG signaling pathway and, in this context, osteoclasts activation could be considered as a protective strategy carried out by the host bone tissue to delimitate the necrotic area and to counteract infection.


Assuntos
Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/fisiopatologia , Ligante RANK/fisiologia , Transdução de Sinais , Adulto , Idoso , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Ligante RANK/genética
2.
Minerva Stomatol ; 63(5): 155-65, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-25047261

RESUMO

AIM: Dental research, especially in the field of oral surgery, showed a strong and significant increase during the last years. This was probably determined by the introduction among the clinicians of different therapeutic protocols through biomaterials engineering, and by the large broadcasting of scientific knowledge due to new media such as the internet. The aim of this work was to analyze the scientific production of a sample of Italian Oral Surgeons from 1998 to 2012. METHODS: The scientific production of 252 Active Members belonging to three Italian associations of Oral Surgery (SIdCO, SIO, SICOI) was examined. The number of authors, the number of publications and the number of citation were quantified for three periods of 5 years each from the first year considered along 15 years. The overall sample was then divided into two groups, academics and not academics, in order to differently assess the scientific production conducted inside or outside the University. RESULTS: Over the years, scientific production increased considerably, with a progression not strictly proportional if compared to the number of authors, especially in the last 5 years. By spearately considering the academics and the not academics authors, the biggest contribution to the scientific production increasingly came from the last 5 years, both in terms of authors' and published papers number. CONCLUSION: The results reported in this bibliometric analysis show how scientific research increasingly pursued by clinicians in oral surgery in the last 15 years.


Assuntos
Editoração/estatística & dados numéricos , Cirurgia Bucal , Bibliometria , Itália , Fatores de Tempo
3.
Eur J Histochem ; 57(1): e10, 2013 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-23549459

RESUMO

Autologous bone is considered the gold standard for bone regeneration, even if different heterologous bone substitutes have been proposed to overcome the limits related to its use. The aim of this study was to analyze and to compare the molecular events switched on by autologous or heterologous bone graft insertion, focusing on TGFß1 expression and OPG/RANKL ratio, to analyze resorption process, and estimating graft vascularization, new bone tissue deposition and its mineralization, through VEGF, BSP and SPARC expression evaluation, respectively. Patients needing pre-prosthetic rehabilitation of the posterior maxilla were treated using an equine-derived biomaterial (Group 1) or calvaria autologous bone (Group 2), according to the morphology of the bone defect. Bone graft integration was evaluated on bone samples obtained from the treated areas at the moment of dental implant insertion, by morphological and immunohistochemical analyses for TGFß1, OPG, RANKL, VEGF, BSP, and SPARC expression. Morphological analysis shows the presence of biomaterial residual granules in Group 1, in parallel to a good integration between graft and host tissue. Moderate TGFß1 expression is seen in both Group 1 and Group 2. OPG/RANKL ratio appear higher in Group 1; VEGF expression appears very strong in Group 1 and strong in Group 2, while BSP and SPARC expression results weak in Group 1 and moderate in Group 2. Results reveal the good integration between both types of graft and the host tissue, even though autologous graft seems to produce a faster regenerative process, as evidenced by the different expression of the investigated molecules. According to these observations, the clinical use of heterologous particulate equine-derived biomaterial may ensure long-term predictability of implant-prosthetic rehabilitation, comparable to that obtained with autologous bone graft.


Assuntos
Substitutos Ósseos/administração & dosagem , Regulação da Expressão Gênica , Cavalos , Maxila , Osteoprotegerina/biossíntese , Ligante RANK/biossíntese , Crânio/química , Animais , Substitutos Ósseos/química , Feminino , Humanos , Masculino , Maxila/anormalidades , Maxila/metabolismo , Maxila/patologia , Maxila/cirurgia , Pessoa de Meia-Idade , Fator de Crescimento Transformador beta1/biossíntese , Transplante Autólogo , Transplante Heterólogo
4.
J Biol Regul Homeost Agents ; 26(2 Suppl): 45-50, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23164326

RESUMO

Dental pulp undergoes a number of changes passing from healthy status to inflammation due to deep decay. These changes are regulated by several genes resulting differently expressed in inflamed and healthy dental pulp, and the knowledge of the processes underlying this differential expression is of great relevance in the identification of the pathogenesis of the disease. In this study, the gene expression profile of inflamed and healthy dental pulps were compared by microarray analysis, and data obtained were analyzed by Ingenuity Pathway Analysis (IPA) software. This analysis allows to focus on a variety of genes, typically expressed in inflamed tissues. The comparison analysis showed an increased expression of several genes in inflamed pulp, among which IL1β and CD40 resulted of particular interest. These results indicate that gene expression profile of human dental pulp in different physiological and pathological conditions may become an useful tool for improving our knowledge about processes regulating pulp inflammation.


Assuntos
Antígenos CD40/fisiologia , Polpa Dentária/metabolismo , Interleucina-1beta/fisiologia , Análise de Sequência com Séries de Oligonucleotídeos , Pulpite/metabolismo , Transcriptoma , Adulto , Antígenos CD40/genética , Humanos , Interleucina-1beta/genética , Pulpite/etiologia
5.
Int J Immunopathol Pharmacol ; 22(4): 1105-16, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-20074475

RESUMO

Bone regeneration procedures allow oral rehabilitation with dental implants also in edentulous ridges with severe bone atrophy. The integration of grafted materials with the host tissue can initiate regenerative, inflammatory and apoptotic response. Since molecular mechanisms exist at the basis of such response, the aim of this work is to investigate, by immunohistochemical analyses, the expression of proteins involved in the graft integration process, in parallel to clinical and histological modifications, occurring on sites treated with extraoral autologous bone graft deriving from the parietal region of the calvaria (eAB), intraoral autologous bone graft deriving from mandibular ramus (iAB) and heterologous bone graft from swine (hB) in human patients. In our study, the immunohistochemical expression of BSP, VEGF, eNOS in eAB samples was significantly higher (p < 0.05) compared to values recorded in iAB and hB samples. The inflammatory response, investigated by iNOS expression, was found lower in all autologous samples (eAB and iAB) compared to hB, at statistically significant values. Moreover, the expression of the pro-apoptotic molecule, Bax, resulted significantly lower (p < 0.05) in eAB than in iAB and hB samples. These values, together with the low number of apoptotic cells detected in autologous samples, suggest a good regenerative response when extraoral autologous bone graft is used in comparison to the response from the other grafts, and also suggest the use of calvaria graft as a predictable therapeutic procedure for repairing severe bone defects in oral and maxillofacial surgery, not only by clinical and biomechanical criteria, but also from a biomolecular aspect.


Assuntos
Perda do Osso Alveolar/cirurgia , Transplante Ósseo/métodos , Mandíbula/transplante , Maxila/cirurgia , Doenças Maxilares/cirurgia , Óxido Nítrico Sintase Tipo III/metabolismo , Osseointegração , Osso Parietal/transplante , Fator A de Crescimento do Endotélio Vascular/metabolismo , Idoso , Perda do Osso Alveolar/enzimologia , Perda do Osso Alveolar/patologia , Animais , Colágeno Tipo I/metabolismo , Feminino , Humanos , Imuno-Histoquímica , Marcação In Situ das Extremidades Cortadas , Mediadores da Inflamação/metabolismo , Sialoproteína de Ligação à Integrina , Masculino , Maxila/enzimologia , Maxila/patologia , Doenças Maxilares/enzimologia , Doenças Maxilares/patologia , Pessoa de Meia-Idade , NF-kappa B/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Osteotomia , Pró-Colágeno/metabolismo , Sialoglicoproteínas/metabolismo , Suínos , Transplante Autólogo , Transplante Heterólogo , Resultado do Tratamento , Proteína X Associada a bcl-2/metabolismo
6.
Int J Immunopathol Pharmacol ; 21(2): 309-18, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18547474

RESUMO

The purpose of this study is to characterise the expression of matrix extracellular phosphoglycoprotein (MEPE) in cultured mesenchymal cells isolated from human dental papilla (PaMCs) of impacted third molars either before or during differentiation of these cells into osteo/odontoblasts. PaMCs, like mesenchymal cells deriving from human dental pulp (DPMCs), resulted positive for a number of mesenchymal markers including CD146 and STRO-1. During the first week in culture they showed a faster proliferation rate than DPMCs, coupled to an earlier down-regulation of MEPE. Also when the cells were further cultured in osteogenic medium (containing beta-glycerophosphate, ascorbic acid and dexamethasone) for 40 days, MEPE down-regulation coupled to an increased expression of osteogenic markers, such as osteocalcin and alkaline phosphatase, occurred earlier in PaMCs than in DPMCs. Thus, our data, indicating that also in PaMCs MEPE expression is higher when cells proliferate, whereas it is downregulated as cells differentiated, are in favour of a role of MEPE as an early regulator of odontogenic differentiation. We also confirm the superior proliferative potential of PaMCs in comparison with DPMCs, coupled to a more rapid induction of osteogenic differentiation. Therefore, these cells represent an optimal source to be conveniently used for dental tissue engineering and tooth regeneration.


Assuntos
Papila Dentária/fisiologia , Proteínas da Matriz Extracelular/biossíntese , Glicoproteínas/biossíntese , Células-Tronco Mesenquimais/fisiologia , Osteoblastos/fisiologia , Osteócitos/fisiologia , Fosfoproteínas/biossíntese , Adolescente , Adulto , Antraquinonas , Antígenos CD/metabolismo , Northern Blotting , Western Blotting , Calcificação Fisiológica/fisiologia , Adesão Celular/fisiologia , Diferenciação Celular/fisiologia , Proliferação de Células , Criança , Citometria de Fluxo , Humanos , Masculino , Células-Tronco Mesenquimais/metabolismo , Osteoblastos/metabolismo , Osteócitos/metabolismo , RNA/biossíntese , RNA/isolamento & purificação
7.
Int J Immunopathol Pharmacol ; 20(1 Suppl 1): 75-9, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17897506

RESUMO

Cancer of the oral cavity is a frequent worldwide neoplasia, averaging 4% in men and 2% in women. Cancer of the oral cavity is often preceded by particular epithelial lesions, called precancerous lesions. The aim of this study is to evaluate, by SEM microscope analysis, before and after treatment with 0.1% 13-cis-retinoic, the morphostructural variation of the surface of the oral mucous affected by lichen planus in respect to healthy oral mucous. Following topical therapy with 0.1% 13-cis-retinoic acid for 8 weeks, a restoration of the physiological morphostructure was observed by SEM, demonstrating the efficaciousness of the treatment and the SEM analysis for identifying the lesions of oral epithelium.


Assuntos
Isotretinoína/uso terapêutico , Líquen Plano Bucal/tratamento farmacológico , Mucosa Bucal/ultraestrutura , Humanos , Líquen Plano Bucal/patologia , Microscopia Eletrônica de Varredura
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...