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1.
Eur J Pharm Biopharm ; 85(1): 130-8, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23680585

RESUMO

Four cement applications were tested in this investigation. Two dicalcium phosphate dihydrate (DCPD-brushite) hydraulic cements, an apatite hydraulic fiber loaded cement, and a calcium sulfate cement (Plaster of Paris) were implanted in epiphyseal and metaphyseal cylindrical bone defects in sheep. The in vivo study was performed to assess the biocompatibility and bone remodeling of four cement formulations. After time periods of 2, 4, and 6 months, the cement samples were clinically and histologically evaluated. Histomorphometrically, the amount of new bone formation, fibrous tissue, and bone marrow and the area of remaining cement were measured. In all specimens, no signs of inflammation were detectable either macroscopically or microscopically. Cements differed mainly in their resorption time. Calcium sulfate was already completely resorbed at 2 months and showed a variable amount of new bone formation and/or fibrous tissue in the original drill hole over all time periods. The two DCPD cements in contrast were degraded to a large amount at 6 months, whereas the apatite was almost unchanged over all time periods.


Assuntos
Materiais Biocompatíveis , Cimentos Ósseos/uso terapêutico , Regeneração Óssea , Remodelação Óssea , Osso e Ossos/cirurgia , Absorção , Animais , Animais Endogâmicos , Materiais Biocompatíveis/efeitos adversos , Cimentos Ósseos/efeitos adversos , Cimentos Ósseos/química , Osso e Ossos/citologia , Osso e Ossos/lesões , Osso e Ossos/fisiologia , Fosfatos de Cálcio/administração & dosagem , Fosfatos de Cálcio/efeitos adversos , Fosfatos de Cálcio/química , Fosfatos de Cálcio/uso terapêutico , Sulfato de Cálcio/administração & dosagem , Sulfato de Cálcio/efeitos adversos , Sulfato de Cálcio/química , Sulfato de Cálcio/uso terapêutico , Epífises/lesões , Epífises/cirurgia , Feminino , Reação a Corpo Estranho/prevenção & controle , Injeções Intralesionais , Carneiro Doméstico , Solubilidade , Fatores de Tempo , Viscosidade
2.
J Vet Diagn Invest ; 23(2): 333-7, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21398458

RESUMO

Because interactions between livestock and chamois occur on Alpine pastures, transmission of infectious diseases is considered possible. Thus, the occurrence of Chlamydiaceae, Mycoplasma conjunctivae, and pestiviruses in Alpine chamois (Rupicapra r. rupicapra) of the Surselva region (eastern Swiss Alps) was investigated. In total, 71 sera, 158 eye swabs, 135 tissue samples, and 23 fecal samples from 85 chamois were analyzed. The sera were tested by 2 enzyme-linked immunosorbent assay (ELISA) kits specific for Chlamydophila abortus. Eye swabs, tissue, and fecal samples were examined by a Chlamydiaceae-specific real-time polymerase chain reaction (PCR). Positive cases were further investigated by microarray method. One serum sample (1.4%) was positive in 1 of the ELISAs. Eye swabs of 3 chamois (3.8%) were positive for Chlamydiaceae. The microarray method revealed the presence of Chlamydophila abortus, C. pecorum, and C. pneumoniae. All tissue and fecal samples were negative. With real-time PCR, 3.9% of the chamois tested positive for Mycoplasma conjunctivae. One chamois had a simultaneous infection with M. conjunctivae and 2 chlamydial species (C. abortus, C. pecorum). Skin and tongue tissue samples of 35 chamois were negative for pestivirus antigen by immunohistochemistry. It was concluded that in contrast to the findings in Pyrenean chamois (Capra p. pyrenaica) of Spain, the occurrence of Chlamydiaceae in Alpine chamois of the Surselva region is low, and the transmission between domestic and wild Caprinae seems not to be frequent. Comparably, persistent pestiviral infections do not seem to be common in chamois of the Surselva region.


Assuntos
Infecções Bacterianas/veterinária , Chlamydiaceae/isolamento & purificação , Mycoplasma conjunctivae/isolamento & purificação , Infecções por Pestivirus/veterinária , Pestivirus/isolamento & purificação , Rupicapra/microbiologia , Animais , Animais Selvagens , Infecções Bacterianas/epidemiologia , Infecções Bacterianas/microbiologia , Chlamydiaceae/genética , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Viral/química , DNA Viral/genética , Mycoplasma conjunctivae/genética , Análise de Sequência com Séries de Oligonucleotídeos , Pestivirus/genética , Infecções por Pestivirus/epidemiologia , Infecções por Pestivirus/virologia , Reação em Cadeia da Polimerase/veterinária , Suíça/epidemiologia
3.
BMC Vet Res ; 5: 11, 2009 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-19335885

RESUMO

BACKGROUND: Two domestic shorthair cats presenting with progressive hind-limb ataxia and increased aggressiveness were necropsied and a post mortem diagnosis of Feline Spongiform Encephalopathy (FSE) was made. A wide spectrum of tissue samples was collected and evaluated histologically and immunohistologically for the presence of PrPSc. RESULTS: Histopathological examination revealed a diffuse vacuolation of the grey matter neuropil with the following areas being most severely affected: corpus geniculatum medialis, thalamus, gyrus dentatus of the hippocampus, corpus striatum, and deep layers of the cerebral and cerebellar cortex as well as in the brain stem. In addition, a diffuse glial reaction involving astrocytes and microglia and intraneuronal vacuolation in a few neurons in the brain stem was present.Heavy PrPSc immunostaining was detected in brain, retina, optic nerve, pars nervosa of the pituitary gland, trigeminal ganglia and small amounts in the myenteric plexus of the small intestine (duodenum, jejunum) and slightly in the medulla of the adrenal gland. CONCLUSION: The PrPSc distribution within the brain was consistent with that described in other FSE-affected cats. The pattern of abnormal PrP in the retina corresponded to that found in a captive cheetah with FSE, in sheep with scrapie and was similar to nvCJD in humans.


Assuntos
Doenças do Gato/patologia , Tecido Nervoso/patologia , Proteínas PrPSc/metabolismo , Doenças Priônicas/veterinária , Animais , Tronco Encefálico/patologia , Doenças do Gato/metabolismo , Gatos , Córtex Cerebral/patologia , Feminino , Imuno-Histoquímica , Masculino , Doenças Priônicas/metabolismo , Doenças Priônicas/patologia
4.
J Vet Diagn Invest ; 19(1): 28-34, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17459829

RESUMO

Five diagnostic techniques performed on skin biopsies (shoulder region) and/or serum were compared for detection of bovine viral diarrhea virus infection in 224 calves 0-3 months of age, 23 calves older than 3 months but younger than 7 months, and 11 cattle older than 7 months. The diagnostic methods used were immunohistochemistry (IHC), 2 commercial antigen ELISAs, 1 commercial antibody ELISA, and real-time RT-PCR. Results of 249 out of 258 skin and serum samples were identical and correlated within the 3 antigen detection methods and the real-time RT-PCR used. Twenty-six of these 249 samples were BVDV-positive with all antigen detection methods and the real-time RT-PCR. Nine out of 258 samples yielding discordant results were additionally examined by RT-PCR, RT-PCR Reamplification (ReA), and antigen ELISA I on serum and by immunohistochemistry on formalin fixed and paraffin-embedded skin biopsies. Virus isolation and genotyping was performed as well on these discordant samples. In 3 cases, transiently infected animals were identified. Two samples positive by real-time RT-PCR were interpreted as false positive and were ascribed to cross-contamination. The antigen ELISA II failed to detect 2 BVDV-positive calves due to the presence of maternal antibodies; the cause of 2 false-positive cases in this ELISA remained undetermined. Only persistently infected animals were identified in skin samples by IHC or antigen ELISA I. The 3 antigen detection methods and the real-time RT-PCR used in parallel had a high correlation rate (96.5%) and similar sensitivity and specificity values.


Assuntos
Doença das Mucosas por Vírus da Diarreia Viral Bovina/diagnóstico , Doença das Mucosas por Vírus da Diarreia Viral Bovina/virologia , Vírus da Diarreia Viral Bovina/isolamento & purificação , Ensaio de Imunoadsorção Enzimática/veterinária , Reação em Cadeia da Polimerase/veterinária , Animais , Anticorpos Antivirais/sangue , Bovinos , Vírus da Diarreia Viral Bovina/genética , Genótipo , Pele/imunologia
5.
Emerg Infect Dis ; 12(4): 675-7, 2006 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-16704819

RESUMO

Borna disease virus (BDV) is the causative agent of severe T-cell-mediated meningoencephalitis in horses, sheep, and other animal species in central Europe. Here we report the first unequivocal detection of a BDV reservoir species, the bicolored white-toothed shrew, Crocidura leucodon, in an area in Switzerland with endemic Borna disease.


Assuntos
Vírus da Doença de Borna/isolamento & purificação , Reservatórios de Doenças/veterinária , Musaranhos/virologia , Animais , Doença de Borna/epidemiologia , Doença de Borna/virologia , Encéfalo/virologia , Reservatórios de Doenças/virologia , Coração/virologia , Camundongos , Toupeiras/virologia , Suíça
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