[Study of the antiviral activity, pharmacokinetics and toxic properties of liposomal form of human alpha-2b interferon administered topically]. / Izuchenie protivovirusnoi aktivnosti, farmakokinetiki i toksicheskikh svoistv liposomal'noi formy rekombinantnogo alfa-2b-interferona cheloveka pri naruzhnom primenenii.

Human liposomal recombinant alpha 2b-interferon topically applied to laboratory animals was tested for antiviral activity, pharmacokinetics, and toxicity. The interferon was shown to penetrate through the skin and to circulate in the blood of experimental animals longer than its injectable form, and to exhibit its antiviral activity against genital herpes in guinea pigs. It produced no toxic, skin-irritant, or allergic effects in laboratory animals.

[Design of human liposomal recombinant alpha2-interferon-containing preparation]. / Sozdanie liposomal'nogo preparata, soderzhashchego chelovecheskii rekombinantnyi alpha2-interferon.

The composition of liposomal lipid phase preparation was selected. The optimum ratio of liposomal lipid phase components was found to produce a a human liposomal recombinant alpha 2-interferon-containing preparation by extrusion across the polycarbonate membranes. The study gave rise to a lyophilized sterile liposomal recombinant alpha 2-interferon preparation. The agent was shown to be stable and retains its antiviral activity within a year.

Application of pattern recognition and image classification techniques to determine continuous cardiac output from the arterial pressure waveform.

The shape of the arterial pressure waveform is a non-linear function of stroke volume, heart rate and many other cardiovascular parameters. Previous attempts have been made to exploit this relationship and derive cardiac output (CO) from the arterial pressure waveform. These classical "pulse-contour" methods utilized simplifying linear assumptions, as a result they failed to adequately estimate CO over a sufficiently wide range of hemodynamic conditions. We have applied pattern recognition and image processing techniques to the problem of deriving CO from the arterial pressure waveform, thereby eliminating the need for simplifying assumptions. Computer simulations were used to develop the basic pattern recognition algorithms and compare their performance with that of published classical "pulse-contour" methods. Animal models were subsequently used to demonstrate proof of the concept. For over 200,000 individual heart beats, covering a wide range of hemodynamic conditions, the mean error, in calculated CO compared to ultrasonic flow probe determined CO, was 2.8% with a standard deviation of 9.8%.

[Methodological approaches to creating a liposomal form of human recombinant alpha 2-interferon]. / Metodichesie podkhody k sozdaniiu liposomal'noi formy chelovecheskogo rekombinantnogo al'fa 2-interferona.

The authors have obtained several variants of liposomal forms of human alpha 2-interferon. The forms intended to preserve antiviral activity with regard to the methods of liposomal formation were comparatively studied. It has been found that liposomal formation through stirring caused no decrease in antiviral activity.

[Changes in the blood serum aminotransferase activity in the experimental infection of Cercopithecus aethiops monkeys with the Marburg virus]. / Izmeneniia aktivnosti aminotransferaz syvorotki krovi pri éksperimental'nom zarazhenii obez'ian Cercopithecus aethiops virusom Marburg.

Aminotransferase levels in the peripheral blood serum of African green monkeys were studied after aerosol infection with lyophilized virus preparation. A correlation between aminotransferase activity and other symptoms of hemorrhagic fever was shown. Considerable variations in the features of aminotransferase activity in severe and mild cases of the disease were observed.