[THE DIAGNOSTICS OF HEREDITARY DISORDERS OF METABOLISM OF PURINES AND PYRIMIDINES IN CHILDREN USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF ELECTRO-SPRAY TANDEM MASS-SPECTROMETRY].

The article presents data concerning new technique of diagnostic of diseases of metabolism of purines and pyrimidines using high performance liquid chromatography combined with electro-spray mass-spectrometry. The procedure of analysis is described in detail: from pre-analytical stage to interpretation of data of liquid chromatography mass-spectrometry, control of quality of data analysis, mass-spectrometry parameters and chromatographic conditions of analysis of purines, pyrimidines and their metabolites. The reference values are presented for purine and pyrimidine nucleosides and bases in urine of healthy individuals. The chemical structure of purines, pyrimidines and their metabolites and examples of chromato-mass-spectrograms under various hereditary disorders of metabolism of purines and pyrimidines are presented as well. The article is targeted to pediatricians of all profiles, medical geneticists and physicians of laboratory diagnostic.

[Study of glutamine solution use efficiency in pediatric patients with heavy thermic burns and concomitant injuries in the intensive care unit].

Comparing analysis of randomized study was conducted. 40 Pediatric patients (2-15 years old) with heavy thermic burns and concomitant injuries were recruited into the study to assess the glutamine solution intravenous use efficiency. Parenteral feeding and feeding with glutamine solution intravenous use were clinicaly and laboratory compared Duration of mechanical ventilation decreased in group of feeding with glutamine solution intravenous use. Preliminary results of the study recommend to include glutamine in the programs of parentreral feeding.

Red cell Na+/H+ exchange and B cell alloantigen 883 (D8/17) in patients with acute rheumatic fever and inactive rheumatic heart disease.

The association of Na+/H+ antiport with 883 alloantigen was studied in patients with rheumatic disease. Simultaneous measurements were made of red cell Na+/H+ exchange and 883 alloantigen in microlymphocytotoxic test with D8/17 monoclonal antibodies in 20 patients with acute rheumatic fever, 20 patients with inactive rheumatic heart disease, 20 patients with atherosclerotic heart disease (stable anginal syndrome), and 20 healthy subjects. The number of 883(+) B cells and the Na+/H+ antiport activity were increased in rheumatic fever compared to healthy controls: 24.8 +/- 0.4 vs. 11.1 +/- 1.0% cells, 431 +/- 43 vs 121 +/- 12 micromol H+/l cells in min, respectively; p < 0.001; and in patients with rheumatic heart disease compared to patients with atherosclerotic heart disease; 25.7 +/- 1.8 vs. 9.8 +/- 1.1% cells, 482 +/- 73 vs. 124 +/- 12 micromol H+/l cells in min, respectively; p < 0.001.

[Detection of B-cell marker of rheumatism using monoclonal antibodies D8/17 in the families of patients with rheumatism]. / Vyiavlenie B-kletochnogo markera revmatizma s pomoshch'iu monoklonal'nykh antitel D8/17 v sem'iakh bol'nykh revmatizmom.

To study hereditary disposition for rheumatism (R) 141 persons including 26 probands of patients with R, 79 relatives and also 12 newborns of mothers with R were examined. The carriage of the B-cell markers was studied by means of antibodies D8/17. The B-cell marker was revealed in 96.2 per cent probands and in 41.4 per cent relatives. Among the female relatives with R the B-cell marker carriage was revealed in 51.2 per cent, among the male relatives in 25.9 per cent. A high carriage of the given marker in children and young people aged 4-34 born of mothers with R was noted (66.7 per cent). Among the newborn no carriers of the B-cell marker were detected. A conclusion has been made of the participation of the B-cell marker in the formation of hereditary predisposition for R.

[Detection of B-cell marker of rheumatism using monoclonal antibodies D8/17 in families of patients with rheumatism (report 1)]. / Vyiavlenie B-kletochnogo markera revmatizma s pomoshch'iu monoklonal'nykh antitel D8/17 v sem'iakh bol'nykh revmatizmom (soobshchenie 1).

Results of clinico-genealogical and immunogenetic examination of 220 persons including 150 patients suffering from rheumatism and members of their families have been presented. The object of the examination was a B-cell marker carriership to be determined by means of monoclonic antibodies D8/17 with the use of the microlymphocytotoxic method. The marker carriership was detected in 96 per cent of probands-patients with rheumatism and in 40.3 per cent of their relatives and in 6.7 per cent of healthy individuals of the control group. A possible contribution of the B-cell marker to rheumatism liability was suggested. A further study of the role of the given marker in rheumatism has been found advisable.