RESUMO
It is known that the addition of methanol to the culture medium stimulates the photosynthetic productivity of some species of microalgae, but its influence on the biochemical composition of the biomass has not been investigated. The aim of the present work is to determine the effect of methanol (50 mM) on the content of free amino acids, soluble proteins and reduced nicotinamide coenzyme NAD(P)H in C. reinhartdii cells. It is shown that in case of illumination of C. reinhardtii methanol raises intracellular content of NAD(P)H four times more efficiently than in the darkness. Total content of free amino acids is increased and their ratio is changed. The concentration of glutamic acid, glutamine, alanine, serine and tyrosine also increases. The concentration of valine and methionine is reduced. Growth of culture with methanol is followed by an increase in the content of intracellular protein by 30% after 20 hours of cultivation. The obtained data indicate that methanol stimulates growth of C. reinhardtii, not only as a result of additional carbon utilization, but also due to improved nitrogen assimilation and the impact on the energy metabolism of cells.
Assuntos
Aminoácidos/metabolismo , Chlamydomonas reinhardtii/efeitos dos fármacos , Chlamydomonas reinhardtii/metabolismo , Metanol/farmacologia , NADP/metabolismo , Proteínas de Plantas/metabolismo , Biomassa , Carbono/metabolismo , Chlamydomonas reinhardtii/crescimento & desenvolvimento , Cromatografia por Troca Iônica , Meios de Cultura , Metabolismo Energético/efeitos dos fármacos , Luz , Nitrogênio/metabolismo , Fotossíntese/efeitos dos fármacos , Proteínas de Plantas/agonistasRESUMO
Methabolism of methanol in plant organisms is considered in the paper. Enzymes of consecutive oxidation of methanol and enzymes responsible for incorporation of carbon from methanol molecule to methyl groups of phospholipids, carboxylic acids and carbohydrates have been described. The peculiarity of plant organisms is in interaction of reactions of methanol transformation with pathways of photorespiration and C1-metabolism and in the capacity to use methanol carbon to form organic matter through photosynthesis. The inclusion of methanol metabolites in anabolic processes occurs at the level of formaldehyde and formiate. As a result, exogenous methanol at low concentrations can stimulate the photosynthetic efficiency of plants.
