[Evaluation of the efficiency of hepatoprotective monotherapy using succinic acid and methionine for nonalcoholic fatty liver disease at the stage of steatohepatitis].

AIM: To evaluate the efficiency of Remaxol monotherapy in patients with nonalcoholic fatty liver disease (NAFLD) at the stage of steatohepatitis (SH). MATERIALS AND METHODS: The treatment of 156 patients with NAFLD at the stage of SH was analyzed. A study group included 84 patients who had received intravenous Remaxol, 400 ml, dropwise at a rate of 40-60 drops per minute once daily in the morning for 10 days; a control group of 72 patients had been treated with the conventional scheme. RESULTS: During the treatment, the study group showed a rapider relief of the manifestations of asthenovegetative and dyspeptic syndromes and a reduction in the magnitude of biochemical manifestations of cytolytic and cholestatic syndromes than did the control group. CONCLUSION: Incorporation of Remaxol into the therapy regimen in patients with NAFLD at the stage of SH enhances the effectiveness of treatment.

[Radionuclide techniques in early diagnosis of pulmonary artery thromboembolism]. / Metody radionuklidnykh issledovanii v rannei diagnostike tromboémbolii vetvei legochnoi arterii.

A combined procedure of indirect radionuclide phlebography and emission tomography of the lung with human serum albumin microspheres was employed to diagnose thromboembolism of pulmonary arterial branches (TPAB) and thrombosis in the inferior cava system. Four hundred and forty nine patients suspected for TPAB were examined. The data characteristic of pulmonary thromboembolism were obtained in 21.6% of cases. In 75 (17.6%) of 432 patients, indirect radionuclide phlebography revealed signs of thrombosis in the inferior cava at various sites. The radionuclide study of pulmonary perfusion is the only technique that assesses the degree of recovery of pulmonary perfusion after TPAB.

[Isolation and characteristics of single-stranded DNA-binding protein (SSB-protein) from Ehrlich ascites carcinoma cells]. / Vydelenie i kharakteristika belka, sviazyvaiushchegosia s odnonitevoi DNK (SSB-belka) iz kletok astsitnoi kartsinomy Erlikha (AKE).

Single-stranded DNA-binding protein (SSB-protein) has been purified and characterized from Ehrlich ascite tumour (EAT) cells. The purification procedure was performed in analytical and preparative variants. It was shown that in the analytical variant of the purification procedure can be used to determine protein concentration in the cell. The molecular mass of the SSB-protein as determined by SDS polyacrylamide gel electrophoresis is 36 and 43 kD; that determined by gel filtration is 27, 28, 43 and 44 kD; pI is 7.4. The use of nitrocellulose filters showed that the SSB-protein binds preferentially to ss-DNA. The protein contains no admixtures of DNA-polymerases, endo- or exonucleases, DNA-dependent ATPase, lactate dehydrogenase and HMG-proteins. The SSB-protein stimulates 1.5-2-fold the activity of DNA-polymerase alpha from EAT, it does not activate DNA-polymerase beta from EAT and strongly inhibits the activity of exonuclease (snake venom phosphodiesterase). The specificity of the term "SSB-protein" which makes it different from other non-histone proteins of chromatin is discussed.