Microparticle prepared by chitosan coating on the extruded mixture of corn starch, resveratrol, and α-amylase controlled the resveratrol release.

Microcapsule was developed by chitosan coating on the microparticle which was prepared by smashing the extruded mixture of corn starch, resveratrol, and α-amylase. In the preparation process, the low-temperature extrusion and α-amylase were employed to overcome the disadvantages of low gelatinization, dissolution, and poor hydration of extruded starch. Chitosan-coating retarded starch aging, improved the stability of microcapsules, delayed the release of resveratrol. Considering the bioactive functions of chitosan, microcapsules also obtained the functions of chitosan by chitosan coating. The chitosan coating and α-amylase addition improved the release ratio of resveratrol. CESRA (chitosan solution (2%) coating on the extruded mixture of corn starch, resveratrol, and α-amylase) released 86.8% resveratrol at 25 °C in six days chasing, and 85.3% resveratrol at 37 °C in 48 h chasing. Chitosan coating slightly improved the free radical scavenging activity of ABTS+. The particle size variation, SEM, XRD, and FT-IR were also employed to investigate the variation of morphology, crystal structure, and chemical composition.

Characterization of sustained-release chitosan film loaded with rutin-ß-cyclodextrin complex and glucoamylase.

ABSTRACT: Edible chitosan film incorporated with rutin-ß-cyclodextrin was developed and characterized. The delivery of rutin was improved via the hydrolyzation function of glucoamylase, and the antioxidant activity of the chitosan film was enhanced by the addition of rutin. Sodium bicarbonate solution at different pHs (pH-adjusting reagent) was employed to afford the mild condition for the incorporated glucoamylase. The enzyme exhibited its hydrolyzation function to improve the release rate of rutin by destabilizing the rutin-ß-cyclodextrin complex (RCC) in chitosan film. The optimum pH of glucoamylase was achieved with 5 mL addition amount of 0.5 mol/L sodium bicarbonate solution, and the glucoamylase improved the radical scavenging ratio of chitosan film. The yellowness of chitosan film was enhanced with the addition of RCC solution. The films prepared without water demonstrated coarse and rough surface, while the water-based films had smoother and even surface as examined by scanning electron microscopy. In contrast, these observations disappeared in the water immersion groups. X-ray diffraction suggested that the hydrolyzation of ß-cyclodextrin and the interlinkage between ß-cyclodextrin and the chitosan chain exerted a negative function on maintaining the crystal structure of pure chitosan film. Further, the destabilization of RCC complex with the glucoamylase activity was evidenced by the absence of peak associated with ß-cyclodextrin as observed from Fourier transform infrared spectra. The enzyme improved the release of rutin and the addition of RCC successfully endowed antioxidant activity to the chitosan film.