Cluster analysis on hypocrellin A content in anamorphs of Shiraia bambusicola / 中国中药杂志

<p><b>OBJECTIVE</b>By examining the colony growth rate, colony texture, substrate mycelium color and separation method of 40 anamorph strains of Shiraia bambusicola to investigate the relationship between these indexes and content of hypocrellin A.</p><p><b>METHOD</b>In UV-spectrophotometry and microscopic observation were applied in the experiments. The results were analyzed by the clustering and variance analysis.</p><p><b>RESULT</b>SPSS clustering analysis showed that the colony characters of S. bambusicola anamorph strains had significant differences with hypocrellin A content.</p><p><b>CONCLUSION</b>In the tested conditions, substrate mycelium color of S. bambusicola anamophs has the most distinct correlation with the yield of hypocrellin A.</p>

Isolation and determination of anamorph of Shiraia bambusicola / 中国中药杂志

<p><b>OBJECTIVE</b>To isolate and identify the anamorph of Shiraia bambusicola.</p><p><b>METHOD</b>Fungus strains were isolated from mature ascospores and stroma. They were identified by means of morphological identification including colony and microscope characteristic, the molecular identification was done by 5.8S-ITS rDNA.</p><p><b>RESULT</b>The strains GZDXIFR-171 and GZDXIFR-181 were isolated and obtained with the separation of different methods, which had the same colony morphology. With the universal primers of the ITS1-5.8S rDNA-ITS2, the 5.8S-ITS rDNA sequence of GZDXIFR-171 and GZDXIFR-181 were obtained by the PCR amplification and sequencing. Compared with the published nucleotide sequence of 5.8S-ITS rDNA in NCBI (National Center for Biotechnology Information), GZDXIFR-171 and GZDXIFR-181 were highly identical with S. bambusicola.</p><p><b>CONCLUSION</b>The isolated strains GZDXIFR-171 and GZDXIFR-181 were confirmed to be the true anamorph of S. bambusicola.</p>

Artificial culturing of Cordyceps cicadidae on living silkworm / 中国中药杂志

<p><b>UNLABELLED</b>To culture silkworm Cordyceps cicadidae artificially.</p><p><b>METHOD</b>Twenty Paecilomyces cicadae were collected and isolated from 8 natural reserves in China and the Cheju Island of Korea. By the impregnation method, their infection to silkworm larvaes and silkworm chrysalises and the synneneta production were studied.</p><p><b>RESULT</b>The results showed that all P. cicadiae strains could infect silkworm larvaes and silkworm chrysalises, and some strains could produce synnenetas.</p><p><b>CONCLUSION</b>The silkworm chrysalis was better than silkworm larvae to culture C. cicadidae.</p>