[Cultivation of microorganisms in micro-volumes of culture media]. / Kul'tivirovanie mikroorganizmov v mikroob'emakh pitatel'noi sredy.

Studies on the cultivation of aerobic microorganisms in microdrops of growth medium, immobilized in a layer of highly dispersed disconnector (Aerosil), were carried out. The dispersion of the growth medium and its immobilization in a layer of Aerosil was found to produce no influence on the morphological, cultural and biochemical properties of Serratia marcescens cells, determined both immediately after treatment with the field and some time after storage of the treated suspensions. On the contrary, the duration of the action of ferromagnetic bodies produced essential influence on the viability of the microorganisms in dispersed growth medium and on the accumulation of cells in the course of subsequent cultivation. The development of cells grown in the microdrops of the nutriuent medium was similar to that of cells grown in submerged cultivation, following the S-shaped curve. With the increase of the seed dose a drop in the initial growth rate occurred and the accumulation of microbial cells increased by the end of the process. The greatest increase of biomass was observed with minimal seed doses. With a rise in the concentration of amino nitrogen in the growth medium the yield of cells increased only till this concentration reached 320 mg%. Under these conditions the specific growth rate also increased. The accumulation of cells was greater and occurred earlier when microorganisms were cultivated in immobilized state in powder.

[Evaluation on monkeys of reactogenicity and effectiveness of the complex immunoglobulin preparation formulation]. / Otsenka na obez'ianakh reaktogennocti i éffektivnosti lekarstvennykh form kompleksnogo immunoglobulinovogo preparata.

Experiments on 75 monkeys, 20--healthy and 75--with diarrhea, showed that ready medicinal forms of the complex immunoglobulin preparation in tablets, capsules or in a dried form (vials) were safe, well tolerated, clinically and bacteriologically effective. When used simultaneously with antiparasitic treatment, this preparation, irrespective of the kind of its ready medicinal form, showed high curative effect (80-90%). The diarrhea ceasing was accompanied by the gradual normalization of intestinal microbiocenosis (an increased concentrations of Escherichia coli and Bifidobacterium, a decreased amount of opportunistic bacteria). The complex method of the treatment of diarrhea in monkeys (antibiotics, trichopol, phage and probiotics) made it possible to achieve curative effect only in 60% of cases, which required the additional course of treatment, namely the use of rehydration therapy.