Conceptual basis for an integrated system for the management of a protected area. Examples from its application in a mediterranean area.

Improving the efficiency of management in protected areas is imperative in a generalized context of limited conservation budgets. However, this is overlooked due to flaws in problem definition, general disregard for cost information, and a lack of suitable tools for measuring costs and management quality. This study describes an innovative methodological framework, implemented in the web application SIGEIN, focused on maximizing the quality of management against its costs, establishing an explicit justification for any decision. The tool integrates, with this aim, a procedure for prioritizing management objects according to a conservation value, modified by a functional criterion; a project management module; and a module for management of continuous assessment. This appraisal associates the relevance of the conservation targets, the efficacy of the methods employed, both resource and personnel investments, and the resulting costs. Preliminary results of a prototypical SIGEIN application on the Site of Community Importance Chafarinas Islands are included.

[Electrode position in elective electrical cardioversion of atrial fibrillation. A randomized study]. / Estudio aleatorizado sobre la posición de electrodos en la cardioversión eléctrica electiva de la fibrilación auricular.

AIM: To compare the effectiveness of left anteroposterior (A-P) and apex-anterior (A-A) electrode position in the electrical cardioversion (ECV) of patients with atrial fibrillation (AF). DESIGN: Randomized clinical trial. LOCATION: ICU of a second-level hospital. PATIENTS AND METHODS: Ambulatory AF patients admitted to ICU for ECV with biphasic shocks. Up to a maximum of 3 shocks (150-200-200 J) are given in the electrode position determined by random numbers, and if sinus rhythm (SR) is not restored, electrode position is changed and 2 additional 200 J shocks are allowed. Both electrode positions are analyzed and compared to determine which one allows restoration of SR with the lowest number of shocks and least energy. RESULTS: Forty-six patients were included in the A-A group, and 45 in the A-P group. Sinus rhythm was restored in 92% of cases, although patients in the A-A group needed a lower number of shocks and less energy: 1 versus 2 shocks (p=0,003) and 150 versus 350 J (p=0.017). Only one out of 5 patients in whom the A-A position had failed was reverted to RS with the A-P position, whereas 10 out of 13 patients in which A-P position had failed were reverted in the A-A position (p=0.038). CONCLUSIONS: The A-A position is more effective in the elective electrical cardioversion of atrial fibrillation, so we recommend this position as the first choice.

[Limitation of therapeutic effort after ICU admission. Analysis of related factors]. / Limitación del esfuerzo terapéutico tras el ingreso en una Unidad de Medicina Intensiva. Análisis de factores asociados.

OBJECTIVE: To analyze the limitation of therapeutic effort (LTE) in our Intensive Care Unit (ICU) and the variables associated with that decision. DESIGN: Prospective cohort study with a follow up of one year after discharge. SETTING: ICU of a second level hospital. PATIENTS: Four hundred and nine patients admitted during a two-year period. MAIN VARIABLES: APACHE II, NEMS, SOFA, quality of life (PAEEC) and mortality. RESULTS: LTE was performed in 49 (12%) patients. This decision was made by general agreement among the care team in 88% of cases and with the family in 73.5%. It was made on day 8 (4-20) with a SOFA score of 9 (4-13). Mortality in the LTE group was 69.4% in ICU, 92% in hospital, and 96% at 6 and 12 months. A logistic regression model showed that the variables associated with LTE were the following: NEMS score >or=30.7 (OR 12; 95% CI 3.7-39, p < 0.001), NEMS 26.6-30.6 (OR 8; 95% CI 2.5-25.6, p = 0.001), APACHE II > 30 (OR 7.6; 95% CI 2-29, p = 0.003), quality of life >or= 7 (OR 4.2; 95% CI 1.1-15, p = 0.03), age >or= 80 (OR 3.7, 95% CI 1.4-9.5, p = 0.007) and medical patient condition (OR 3.5; 95% CI 1.5-8, p = 0.003). CONCLUSIONS: LTE is a common practice and is usually performed among the care team and the patient's surrogates. The main variables associated with LSC are those related to the severity of illness, previous quality of life, medical disease and patient's age.

Limitación del esfuerzo terapéutico tras el ingreso en una Unidad de Medicina Intensiva. Análisis de factores asociados / Limitation of therapeutic effort after ICU admission. Analysis of related factors

Objetivo. Analizar la limitación del esfuerzo terapéutico (LET) en una Unidad de Medicina Intensiva (UMI) y las variables consideradas en la toma de decisiones. Diseño. Estudio de cohortes prospectivo con seguimiento durante un año tras el alta. Ámbito. UMI de un Hospital de Nivel II. Pacientes. Cuatrocientos nueve pacientes ingresados durante un período de dos años. Variables de interés. APACHE II, Nine Equivalents of Nursing Manpower Use Score (NEMS), Sequential Organ Failure Assessment Score (SOFA), calidad de vida (PAEEC) y mortalidad. Resultados. Se realizó LET en 49 (12%) pacientes. Hubo consenso del equipo asistencial en el 88% de los casos y en el 73,5% con la familia. La decisión se tomó el día 8 (4-20) de estancia y con un SOFA de 9 (4-13) puntos. Entre los pacientes con LET la mortalidad en la UMI fue del 69,4%, la hospitalaria del 92% y a 6 y 12 meses del 96%. Un modelo de regresión logística mostró que las variables asociadas a la decisión de LET fueron las siguientes: NEMS ≥ 30,7 (odds ratio [OR] 12; intervalo de confianza [IC] 95% 3,7-39, p 30 (OR 7,6; IC 95% 2-29, p = 0,003), la calidad de vida previa ≥ 7 (OR 4,2; IC 95% 1,1-15, p = 0,03), edad ≥ 80 años (OR 3,7; IC 95% 1,4-9,5, p = 0,007), paciente médico (OR 3,5; IC 95% 1,5-8, p = 0,003). Conclusiones. La LET es una práctica frecuente y que se realiza generalmente con acuerdo del equipo asistencial y los representantes del paciente. Las variables asociadas a la decisión de LET son las relacionadas con la gravedad, la calidad de vida previa, la patología médica y la edad del paciente

Objective. To analyze the limitation of therapeutic effort (LTE) in our Intensive Care Unit (ICU) and the variables associated with that decision. Design. Prospective cohort study with a follow up of one year after discharge. Setting. ICU of a second level hospital. Patients. Four hundred and nine patients admitted during a two-year period. Main variables. APACHE II, NEMS, SOFA, quality of life (PAEEC) and mortality. Results. LTE was performed in 49 (12%) patients. This decision was made by general agreement among the care team in 88% of cases and with the family in 73.5%. It was made on day 8 (4-20) with a SOFA score of 9 (4-13). Mortality in the LTE group was 69.4% in ICU, 92% in hospital, and 96% at 6 and 12 months. A logistic regression model showed that the variables associated with LTE were the following: NEMS score ≥30.7 (OR 12; 95% CI 3.7-39, p 30 (OR 7.6; 95% CI 2-29, p = 0.003), quality of life ≥ 7 (OR 4.2; 95% CI 1.1-15, p = 0.03), age ≥ 80 (OR 3.7, 95% CI 1.4-9.5, p = 0.007) and medical patient condition (OR 3.5; 95% CI 1.5-8, p = 0.003). Conclusions. LTE is a common practice and is usually performed among the care team and the patient's surrogates. The main variables associated with LSC are those related to the severity of illness, previous quality of life, medical disease and patient's age

early bolting in short days: an Arabidopsis mutation that causes early flowering and partially suppresses the floral phenotype of leafy.

The time of flowering in Arabidopsis is controlled by multiple endogenous and environmental signals. Some of these signals promote the onset of flowering, whereas others repress it. We describe here the isolation and characterization of two allelic mutations that cause early flowering and define a new locus, EARLY BOLTING IN SHORT DAYS (EBS). Acceleration of flowering time in the ebs mutants is especially conspicuous under short-day photoperiods and results from a reduction of the adult vegetative phase of the plants. In addition to the early flowering phenotype, ebs mutants show a reduction in seed dormancy, plant size, and fertility. Double mutant analysis with gibberellin-deficient mutants indicates that both the early-flowering and the precocious-germination phenotypes require gibberellin biosynthesis. Analysis of the genetic interactions among ebs and several mutations causing late flowering shows that the ft mutant phenotype is epistatic over the early flowering of ebs mutants, suggesting that the precocious flowering of ebs requires the FT gene product. Finally, the ebs mutation causes an increase in the level of expression of the floral homeotic genes APETALA3 (AP3), PISTILLATA (PI), and AGAMOUS (AG) and partially rescues the mutant floral phenotype of leafy-6 (lfy-6) mutants. These results suggest that EBS participates as a negative regulator in developmental processes such as germination, flowering induction, and flower organ specification.

Identification of genes specifically expressed in cauliflower reproductive meristems. Molecular characterization of BoREM1.

Using the meristems of the cauliflower curd as a source of tissue and a series of subtractive hybridizations and amplification reactions, we have constructed a cDNA library highly enriched in cDNAs expressed in reproductive meristems. The analysis of a sample of 250 clones from this library identified 22 cDNA clones corresponding to genes specifically expressed in these cauliflower meristems. Apart from two clones that corresponded to APETALA1, and two other ones showing similarity to different aminoacyl-tRNA synthetases, the remaining clones showed no similarity to any sequence in the databases and may correspond to novel genes. One of these clones, BoREM1, was further characterized and found to correspond to a gene encoding a protein with features of regulatory proteins that follows a expression pattern very similar to the LEAFY transcripts.

The (8-thiotheophyllinate) (triphenylphosphine) gold (I), (tTAuP): a new gold complex as an anticancer agent.

In continuous Friend leukemia cell exposure to tTAuP, the IC50 was 0.2 microM whereas in cells exposed 15 or 60 min to tTAuP followed by 72 h in drug-free medium the IC50 was 2.2 and 1.3 microM respectively. A combination of tTAuP and cisplatin (CDDP) is shown to be more active than either agent alone. Intracellular accumulation studies analysed by SXRF have shown that to achieve a cytotoxic effect, large concentrations of gold are necessary to accumulate in both the nuclear and cytoplasmic fraction. However, at cytostatic doses (1 microM), tTAuP has no effect on the cell cycle but does affect DNA synthesis. At a higher dose, greater than that necessary to induce cytotoxicity, it produces DNA damage, as observed by alkaline elution method. When cells were exposed to toxic doses of tTAuP (10 microM) in the presence of albumin, cytotoxicity was significantly reduced. Similar results were obtained when cells were co-treated with L-cysteine, dithiothreitol or reduced gluthatione. Reduced cytotoxic effect can be related to the interaction with free thiol groups. According to these data it is concluded that tTAuP is highly effective in vitro; whether it is active in vivo remains to be determined.

Intracellular accumulation and cytotoxic effect of (8-thiotheophyllinate) (triphenylphosphine) gold(I) in Friend leukemia cells.

Following continuous exposure to tTAuP in medium containing 10% fetal calf serum (FCS), cells resistant to doxorubicin (DOX-RFLC) were 3 fold more resistant to tTAuP than its sensitive counterpart (FLC). Moreover, FLC were 100 fold more sensitive to tTAuP when the cells were grown in the absence of FCS but in the presence of synthetic medium (BMS). When the cytotoxic effect was measured after short-term exposure (60 min) followed by 72 hr incubation in drug-free medium, unexpectedly, DOX-RFLC were 8 fold more sensitive [ID50 = 1.5 microM] than FLC [ID50 = 12 microM] to tTAuP. When FLC were exposed 60 min at different temperatures (4, 25 or 37 degrees C) to tTAuP prior to 72 hr incubation in drug free medium, the ID50 was achieved at 20, 13 and 3 microM respectively). In contrast, when DOX-RFLC were treated in similar conditions, the cytotoxic activity of tTAuP did not vary following exposure at 4, 25 or 37 degrees C. Although the mechanism of action of gold compounds is still unknown we have assumed that tTAuP cytotoxicity is related to the ease with which it is accumulated. This assumption was supported by the analysis of tTAuP incorporation by SXRF. The results reported here show that tTAuP accumulates rapidly in the cell and is distributed in the cytoplasmic and the nuclear fractions. It is suggested that accumulation is mediated by passive diffusion. However, the detection of gold binding to DNA in complexes resistant to solvent treatments suggests that interaction with macromolecules can be mediated by the formation of covalently bound complexes.